################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AccII AccIII AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BalI BbeI BbrPI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BstBI BstEII BstPI BstUI CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1174 GGTACC G^GTACC AccI 475 GTATAC GT^[AC][GT]AC AclI 1023 AACGTT AA^CGTT AcyI 880 GGCGTC G[GA]^CG[CT]C AocI 526 CCTCAGG CC^T[ACGT]AGG Asp718I 1174 GGTACC G^GTACC AspHI 213 GAGCAC G[AT]GC[AT]^C AspI 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 648 GGATCC G^GATCC BbvCI 660 GCTGAGG CCTCAGC(-5/-2) BcgI 351 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 2011 CTAG C^TAG BglII 2036 AGATCT A^GATCT BsaHI 880 GGCGTC G[GA]^CG[CT]C BseRI 1478 GAGGAG GAGGAG(10/8) BsiEI 702 CGACCG CG[GA][CT]^CG BsiHKAI 213 GAGCAC G[AT]GC[AT]^C BsmBI 514 GAGACG CGTCTC(1/5) BsmI 324 GAATGC GAATGC(1/-1) BspMI 1765 ACCTGC ACCTGC(4/8) BsrFI 2044 ACCGGC [GA]^CCGG[CT] BssSI 253 CACGAG CACGAG(-5/-1) Bst1107I 475 GTATAC GTA^TAC BstZ17I 475 GTATAC GTA^TAC Bsu36I 526 CCTCAGG CC^T[ACGT]AGG Cfr10I 2044 ACCGGC [GA]^CCGG[CT] Eco81I 526 CCTCAGG CC^T[ACGT]AGG EcoNI 1297 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1232 GAATTC G^AATTC HaeII 2184 AGCGCC [GA]GCGC^[CT] Hin1I 880 GGCGTC G[GA]^CG[CT]C HincII 610 GTCAAC GT[CT]^[GA]AC HindII 610 GTCAAC GT[CT]^[GA]AC KpnI 1174 GGTACC GGTAC^C MaeI 2011 CTAG C^TAG NdeI 1074 CATATG CA^TATG NspI 1868 GCATGT [GA]CATG^[CT] PflFI 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 787 GACTC GAGTC(4/5) PpuMI 1601 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 1023 AACGTT AA^CGTT PvuII 401 CAGCTG CAG^CTG SexAI 1598 ACCAGGT A^CC[AT]GGT SfcI 724 CTACAG C^T[GA][CT]AG Tth111I 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaI 1088 CCCGGG C^[CT]CG[GA]G 1170 CCCGGG BanII 423 GAGCCC G[GA]GC[CT]^C 1530 GAGCCC BsgI 903 CTGCAC GTGCAG(16/14) 2057 GTGCAG BsoBI 1088 CCCGGG C^[CT]CG[GA]G 1170 CCCGGG BspHI 173 TCATGA T^CATGA 1616 TCATGA BstAPI 328 GCAGATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 854 GCAACTTCTGC BstYI 648 GGATCC [GA]^GATC[CT] 2036 AGATCT DsaI 738 CCATGG C^C[GA][CT]GG 2021 CCATGG HgaI 1645 GACGC GACGC(5/10) 881 GCGTC MaeII 970 ACGT A^CGT 1024 ACGT MflI 648 GGATCC [GA]^GATC[CT] 2036 AGATCT MseI 104 TTAA T^TAA 2162 TTAA NcoI 738 CCATGG C^CATGG 2021 CCATGG PflMI 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1883 CCAATCTCTGG RcaI 173 TCATGA T^CATGA 1616 TCATGA SapI 2211 GAAGAGC GCTCTTC(1/4) 2238 GAAGAGC SmaI 1088 CCCGGG CCC^GGG 1170 CCCGGG SspI 56 AATATT AAT^ATT 65 AATATT Tru9I 104 TTAA T^TAA 2162 TTAA Van91I 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1883 CCAATCTCTGG XcmI 755 CCATCCCATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1574 CCAGAGAGGAAGTGG XhoII 648 GGATCC [GA]^GATC[CT] 2036 AGATCT XmaCI 1088 CCCGGG C^CCGGG 1170 CCCGGG XmaI 1088 CCCGGG C^CCGGG 1170 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI XmnI