(Circular) (Six-base) MAPSORT of: pFN21AE3303.seq Check: 8687 from: 1 to: 6961 pFN21AE3303 6961 bp With 182 enzymes: SgfI * March 28, 2012 12:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4771 278 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 4304 1937 Size: 544 1823 4594 Fragments arranged by size: 4594 1823 544 Acc65I G'GTAC_C Cuts at: 3179 4282 3179 Size: 1103 5858 AclI AA'CG_TT Cuts at: 1563 3029 5088 5461 1563 Size: 1466 2059 373 3063 Fragments arranged by size: 3063 2059 1466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2430 2507 4076 5024 6408 6676 1632 Size: 592 206 77 1569 948 1384 268 1917 Fragments arranged by size: 1917 1569 1384 948 592 268 206 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6764 AflIII A'CryG_T Cuts at: 1203 5860 6837 1203 Size: 4657 977 1327 Fragments arranged by size: 4657 1327 977 AgeI A'CCGG_T Cuts at: 5792 5792 Size: 6961 AhdI GACnn_n'nnGTC Cuts at: 5690 5690 Size: 6961 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6961 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3787 1184 Size: 2603 4358 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 4234 4266 2430 Size: 32 1772 32 5125 Fragments arranged by size: 5125 1772 32 32 AlwNI CAG_nnn'CTG Cuts at: 2215 2644 3431 4704 6276 2215 Size: 429 787 1273 1572 2900 Fragments arranged by size: 2900 1572 1273 787 429 ApaLI G'TGCA_C Cuts at: 5018 6174 5018 Size: 1156 5805 ApoI r'AATT_y Cuts at: 1493 2042 2105 3237 3248 3701 3740 3968 4270 Size: 549 63 1132 11 453 39 228 302 Cuts at: 4270 4496 4648 6757 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1132 549 453 302 228 226 152 63 39 11 AseI AT'TA_AT Cuts at: 160 1032 5515 160 Size: 872 4483 1606 Fragments arranged by size: 4483 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 AvaI C'yCGr_G Cuts at: 1870 1943 3093 3175 4286 1870 Size: 73 1150 82 1111 4545 Fragments arranged by size: 4545 1150 1111 82 73 BamHI G'GATC_C Cuts at: 2653 4291 4658 2653 Size: 1638 367 4956 Fragments arranged by size: 4956 1638 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3179 3746 3884 4282 5737 Size: 325 217 627 1392 567 138 398 1455 Cuts at: 5737 618 Size: 1842 Fragments arranged by size: 1842 1455 1392 627 567 398 325 217 138 BanII G_rGCy'C Cuts at: 1391 2432 3539 4280 1391 Size: 1041 1107 741 4072 Fragments arranged by size: 4072 1107 1041 741 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 2418 2448 928 Size: 971 259 260 30 5441 Fragments arranged by size: 5441 971 260 259 30 BbvCI CC'TCA_GC Cuts at: 2666 2666 Size: 6961 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2345 2379 5152 5186 1903 Size: 34 408 34 2773 34 3678 Fragments arranged by size: 3678 2773 408 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4853 6063 4853 Size: 1210 5751 BclI T'GATC_A Cuts at: 1548 1548 Size: 6961 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5572 136 Size: 107 122 71 631 605 3900 1525 Fragments arranged by size: 3900 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4041 6956 4041 Size: 2915 4046 BlpI GC'TnA_GC Cuts at: 4378 4378 Size: 6961 Bme1580I G_kGCm'C Cuts at: 1790 2368 5022 6178 1790 Size: 578 2654 1156 2573 Fragments arranged by size: 2654 2573 1156 578 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6961 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2468 2632 4075 Size: 416 53 83 399 24 1051 164 1443 Cuts at: 4075 4692 5650 442 Size: 617 958 1753 Fragments arranged by size: 1753 1443 1051 958 617 416 399 164 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6961 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2019 2039 2213 2537 2564 2666 3291 3318 Size: 780 20 174 324 27 102 625 27 Cuts at: 3318 5621 1239 Size: 2303 2579 Fragments arranged by size: 2579 2303 780 625 324 174 102 27 27 20 Bpu10I CC'TnA_GC Cuts at: 2666 3827 2666 Size: 1161 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5086 5951 6249 5086 Size: 865 298 5798 Fragments arranged by size: 5798 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2029 2255 2588 3048 5624 882 Size: 617 530 226 333 460 2576 2219 Fragments arranged by size: 2576 2219 617 530 460 333 226 BsaAI yAC'GTr Cuts at: 493 1204 4752 493 Size: 711 3548 2702 Fragments arranged by size: 3548 2702 711 BsaBI GATnn'nnATC Cuts at: 6955 6955 Size: 6961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2886 4768 5150 5785 Size: 53 83 186 936 1353 1882 382 635 Cuts at: 5785 275 Size: 1451 Fragments arranged by size: 1882 1451 1353 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5394 5792 6066 6213 6624 5394 Size: 398 274 147 411 5731 Fragments arranged by size: 5731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 2783 2813 3620 Size: 30 165 30 1134 30 216 30 807 Cuts at: 3620 3650 1178 Size: 30 4489 Fragments arranged by size: 4489 1134 807 216 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3498 1186 Size: 2312 4649 BseYI C'CCAG_C Cuts at: 2208 3477 3869 6164 6660 2208 Size: 1269 392 2295 496 2509 Fragments arranged by size: 2509 2295 1269 496 392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2893 4083 1163 Size: 573 1157 1190 4041 Fragments arranged by size: 4041 1190 1157 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2710 4411 5172 6200 1540 Size: 275 186 709 1701 761 1028 2301 Fragments arranged by size: 2301 1701 1028 761 709 275 186 BsiHKAI G_wGCw'C Cuts at: 2222 5022 5107 6178 2222 Size: 2800 85 1071 3005 Fragments arranged by size: 3005 2800 1071 85 BsmI GAATG_Cn' Cuts at: 2335 4472 4565 2335 Size: 2137 93 4731 Fragments arranged by size: 4731 2137 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2513 2513 Size: 6961 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2222 2368 2432 3539 4280 5022 5107 Size: 399 432 146 64 1107 741 742 85 Cuts at: 5107 6178 1391 Size: 1071 2174 Fragments arranged by size: 2174 1107 1071 742 741 432 399 146 85 64 BspEI T'CCGG_A Cuts at: 6624 6624 Size: 6961 BspHI T'CATG_A Cuts at: 2178 3621 4850 6580 2178 Size: 1443 1229 1730 2559 Fragments arranged by size: 2559 1730 1443 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3779 4316 844 Size: 2935 537 3489 Fragments arranged by size: 3489 2935 537 BsrBI CCG'CTC Cuts at: 1394 1823 4848 1394 Size: 429 3025 3507 Fragments arranged by size: 3507 3025 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2765 3657 3849 5456 5630 65 Size: 1183 1517 892 192 1607 174 1396 Fragments arranged by size: 1607 1517 1396 1183 892 192 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4049 5605 5792 1700 Size: 110 2239 1556 187 2869 Fragments arranged by size: 2869 2239 1556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6961 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6961 BssSI C'ACGA_G Cuts at: 2258 5021 6033 2258 Size: 2763 1012 3186 Fragments arranged by size: 3186 2763 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2339 2865 794 Size: 1545 526 4890 Fragments arranged by size: 4890 1545 526 BstBI TT'CG_AA Cuts at: 5765 5765 Size: 6961 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6961 BstXI CCAn_nnnn'nTGG Cuts at: 3646 4033 4201 3646 Size: 387 168 6406 Fragments arranged by size: 6406 387 168 BstYI r'GATC_y Cuts at: 1972 2653 4041 4291 4658 5043 5060 6512 6598 Size: 681 1388 250 367 385 17 1452 86 Cuts at: 6598 6610 6956 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1388 681 385 367 346 250 86 17 12 BstZ17I GTA'TAC Cuts at: 2482 2482 Size: 6961 Bsu36I CC'TnA_GG Cuts at: 2532 2532 Size: 6961 BtgI C'CryG_G Cuts at: 513 1065 2004 2743 4026 513 Size: 552 939 739 1283 3448 Fragments arranged by size: 3448 1283 939 739 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6222 Fragments arranged by size: 6222 683 56 BtsI GCAGTG_nn' Cuts at: 4477 5271 5291 4477 Size: 794 20 6147 Fragments arranged by size: 6147 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6961 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2977 3012 4110 4145 529 Size: 35 2413 35 1098 35 3345 Fragments arranged by size: 3345 2413 1098 35 35 35 DraI TTT'AAA Cuts at: 4265 4265 Size: 6961 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5968 809 Size: 507 4652 1802 Fragments arranged by size: 4652 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4408 5297 6926 Size: 54 1425 50 275 18 2578 889 1629 Cuts at: 6926 8 Size: 43 Fragments arranged by size: 2578 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4408 1537 Size: 275 2596 4090 Fragments arranged by size: 4090 2596 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2234 4052 4211 4238 4891 1353 Size: 881 1818 159 27 653 3423 Fragments arranged by size: 3423 1818 881 653 159 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3121 5548 5922 6068 387 Size: 1064 138 1532 2427 374 146 1280 Fragments arranged by size: 2427 1532 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2019 2039 2213 2224 2430 2507 2537 Size: 393 387 20 174 11 206 77 30 Cuts at: 2537 2564 2666 3291 3318 4076 5024 5621 6408 Size: 27 102 625 27 758 948 597 787 Cuts at: 6408 6676 1239 Size: 268 1524 Fragments arranged by size: 1524 948 787 758 625 597 393 387 268 206 174 102 77 30 27 27 20 11 EcoNI CCTnn'n_nnAGG Cuts at: 3306 3306 Size: 6961 EcoO109I rG'GnC_Cy Cuts at: 3299 3607 4205 3299 Size: 308 598 6055 Fragments arranged by size: 6055 598 308 EcoRI G'AATT_C Cuts at: 3237 4270 4648 3237 Size: 1033 378 5550 Fragments arranged by size: 5550 1033 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3424 4240 3424 Size: 816 6145 FspI TGC'GCA Cuts at: 5467 5467 Size: 6961 HaeII r_GCGC'y Cuts at: 1678 4193 6108 1678 Size: 2515 1915 2531 Fragments arranged by size: 2531 2515 1915 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2783 2815 3045 Size: 32 135 32 791 32 388 32 230 Cuts at: 3045 3077 4192 4224 5602 5634 5676 5708 1373 Size: 32 1115 32 1378 32 42 32 2626 Fragments arranged by size: 2626 1378 1115 791 388 230 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2617 4305 4551 669 Size: 988 281 679 1688 246 3079 Fragments arranged by size: 3079 1688 988 679 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6961 HpaI GTT'AAC Cuts at: 4551 4551 Size: 6961 KpnI G_GTAC'C Cuts at: 3183 4286 3183 Size: 1103 5858 MfeI C'AATT_G Cuts at: 4560 4560 Size: 6961 MluI A'CGCG_T Cuts at: 6837 6837 Size: 6961 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3780 6075 6259 1305 Size: 2475 2295 184 2007 Fragments arranged by size: 2475 2295 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5139 Fragments arranged by size: 5139 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 3644 4031 4199 4919 5278 5437 Size: 648 1713 765 387 168 720 359 159 Cuts at: 5437 518 Size: 2042 Fragments arranged by size: 2042 1713 765 720 648 387 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2408 3477 3558 4377 5054 6202 6447 1606 Size: 802 1069 81 819 677 1148 245 2120 Fragments arranged by size: 2120 1148 1069 819 802 677 245 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2743 4026 513 Size: 552 939 739 1283 3448 Fragments arranged by size: 3448 1283 939 739 552 NdeI CA'TA_TG Cuts at: 387 3080 387 Size: 2693 4268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6961 NotI GC'GGCC_GC Cuts at: 4408 4408 Size: 6961 NspI r_CATG'y Cuts at: 1132 1229 3877 4319 5864 1132 Size: 97 2648 442 1545 2229 Fragments arranged by size: 2648 2229 1545 442 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6961 PciI A'CATG_T Cuts at: 5860 5860 Size: 6961 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 3894 1108 Size: 1536 1250 4175 Fragments arranged by size: 4175 1536 1250 PfoI T'CCnGG_A Cuts at: 2586 3840 3927 2586 Size: 1254 87 5620 Fragments arranged by size: 5620 1254 87 PmeI GTTT'AAAC Cuts at: 4265 4265 Size: 6961 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5030 5062 6543 6575 5030 Size: 32 1481 32 5416 Fragments arranged by size: 5416 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3607 3607 Size: 6961 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6961 PsiI TTA'TAA Cuts at: 4531 4531 Size: 6961 PstI C_TGCA'G Cuts at: 830 4313 830 Size: 3483 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6961 PvuII CAG'CTG Cuts at: 2408 2408 Size: 6961 SalI G'TCGA_C Cuts at: 1936 4303 1936 Size: 2367 4594 SapI GCTCTTCn'nnn_ Cuts at: 4211 4238 4211 Size: 27 6934 SbfI CC_TGCA'GG Cuts at: 4313 4313 Size: 6961 ScaI AGT'ACT Cuts at: 5209 6921 5209 Size: 1712 5249 SexAI A'CCwGG_T Cuts at: 3603 3603 Size: 6961 SfcI C'TryA_G Cuts at: 826 1045 2729 4309 5444 6125 6316 826 Size: 219 1684 1580 1135 681 191 1471 Fragments arranged by size: 1684 1580 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6961 SmaI CCC'GGG Cuts at: 1872 3095 3177 4288 1872 Size: 1223 82 1111 4545 Fragments arranged by size: 4545 1223 1111 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 5065 5966 6228 820 Size: 197 926 3122 901 262 1553 Fragments arranged by size: 3122 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4752 493 Size: 4259 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6961 SphI G_CATG'C Cuts at: 1132 4319 1132 Size: 3187 3774 SspI AAT'ATT Cuts at: 5 52 2063 2072 4885 5 Size: 47 2011 9 2813 2081 Fragments arranged by size: 2813 2081 2011 47 9 StuI AGG'CCT Cuts at: 1517 2360 2530 2671 2901 1517 Size: 843 170 141 230 5577 Fragments arranged by size: 5577 843 230 170 141 StyI C'CwwG_G Cuts at: 513 1065 2004 2743 2802 3135 3540 4026 4400 Size: 552 939 739 59 333 405 486 374 Cuts at: 4400 513 Size: 3074 Fragments arranged by size: 3074 939 739 552 486 405 374 333 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2442 4988 5005 5158 5343 5788 Size: 1255 417 445 2546 17 153 185 445 Cuts at: 5788 325 Size: 1498 Fragments arranged by size: 2546 1498 1255 445 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5207 6919 96 Size: 274 80 33 51 1443 3230 1712 138 Fragments arranged by size: 3230 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 3654 3686 6921 6953 2483 Size: 32 1139 32 3235 32 2491 Fragments arranged by size: 3235 2491 1139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3605 1316 Size: 2289 4672 XbaI T'CTAG_A Cuts at: 4297 4297 Size: 6961 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 3586 2767 Size: 819 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6961 XmaI C'CCGG_G Cuts at: 1870 3093 3175 4286 1870 Size: 1223 82 1111 4545 Fragments arranged by size: 4545 1223 1111 82 XmnI GAAnn'nnTTC Cuts at: 2837 3237 3320 5090 2837 Size: 400 83 1770 4708 Fragments arranged by size: 4708 1770 400 83 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4769 276 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI