(Linear) (Six-base) MAPSORT of: KIEE3303.seq Check: 7397 from: 1 to: 2256 KIEE3303.orf With 182 enzymes: SgfI * March 22, 2012 16:29 .. AccI GT'mk_AC Cuts at: 0 476 2256 Size: 476 1780 Acc65I G'GTAC_C Cuts at: 0 1174 2256 Size: 1174 1082 AclI AA'CG_TT Cuts at: 0 1024 2256 Size: 1024 1232 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 425 502 2071 2256 Size: 219 206 77 1569 185 Fragments arranged by size: 1569 219 206 185 77 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1782 2256 Size: 1782 474 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 425 457 2229 2256 Size: 425 32 1772 27 Fragments arranged by size: 1772 425 32 27 AlwNI CAG_nnn'CTG Cuts at: 0 210 639 1426 2256 Size: 210 429 787 830 Fragments arranged by size: 830 787 429 210 ApoI r'AATT_y Cuts at: 0 37 100 1232 1243 1696 1735 1963 2256 Size: 37 63 1132 11 453 39 228 293 Fragments arranged by size: 1132 453 293 228 63 39 37 11 AvaI C'yCGr_G Cuts at: 0 1088 1170 2256 Size: 1088 82 1086 Fragments arranged by size: 1088 1086 82 BamHI G'GATC_C Cuts at: 0 648 2256 Size: 648 1608 BanI G'GyrC_C Cuts at: 0 1174 1741 1879 2256 Size: 1174 567 138 377 Fragments arranged by size: 1174 567 377 138 BanII G_rGCy'C Cuts at: 0 427 1534 2256 Size: 427 1107 722 Fragments arranged by size: 1107 722 427 BbsI GAAGACnn'nnnn_ Cuts at: 0 153 413 443 2256 Size: 153 260 30 1813 Fragments arranged by size: 1813 260 153 30 BbvCI CC'TCA_GC Cuts at: 0 661 2256 Size: 661 1595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 340 374 2256 Size: 340 34 1882 Fragments arranged by size: 1882 340 34 BglII A'GATC_T Cuts at: 0 2036 2256 Size: 2036 220 Bme1580I G_kGCm'C Cuts at: 0 363 2256 Size: 363 1893 BmrI ACTGGGnnnn_n' Cuts at: 0 463 627 2070 2256 Size: 463 164 1443 186 Fragments arranged by size: 1443 463 186 164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 14 34 208 532 559 661 1286 1313 Size: 14 20 174 324 27 102 625 27 Cuts at: 1313 2256 Size: 943 Fragments arranged by size: 943 625 324 174 102 27 27 20 14 Bpu10I CC'TnA_GC Cuts at: 0 661 1822 2256 Size: 661 1161 434 Fragments arranged by size: 1161 661 434 BsaI GGTCTCn'nnnn_ Cuts at: 0 24 250 583 1043 2256 Size: 24 226 333 460 1213 Fragments arranged by size: 1213 460 333 226 24 BsaHI Gr'CG_yC Cuts at: 0 881 2256 Size: 881 1375 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 532 562 778 808 1615 1645 2256 Size: 532 30 216 30 807 30 611 Fragments arranged by size: 807 611 532 216 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1493 2256 Size: 1493 763 BseYI C'CCAG_C Cuts at: 0 203 1472 1864 2256 Size: 203 1269 392 392 Fragments arranged by size: 1269 392 392 203 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 888 2078 2256 Size: 888 1190 178 Fragments arranged by size: 1190 888 178 BsiEI CG_ry'CG Cuts at: 0 705 2256 Size: 705 1551 BsiHKAI G_wGCw'C Cuts at: 0 217 2256 Size: 217 2039 BsmI GAATG_Cn' Cuts at: 0 330 2256 Size: 330 1926 BsmBI CGTCTCn'nnnn_ Cuts at: 0 508 2256 Size: 508 1748 Bsp1286I G_dGCh'C Cuts at: 0 217 363 427 1534 2256 Size: 217 146 64 1107 722 Fragments arranged by size: 1107 722 217 146 64 BspHI T'CATG_A Cuts at: 0 173 1616 2256 Size: 173 1443 640 Fragments arranged by size: 1443 640 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1774 2256 Size: 1774 482 BsrDI GCAATG_nn' Cuts at: 0 760 1652 1844 2256 Size: 760 892 192 412 Fragments arranged by size: 892 760 412 192 BsrFI r'CCGG_y Cuts at: 0 2044 2256 Size: 2044 212 BssSI C'ACGA_G Cuts at: 0 253 2256 Size: 253 2003 BstAPI GCAn_nnn'nTGC Cuts at: 0 334 860 2256 Size: 334 526 1396 Fragments arranged by size: 1396 526 334 BstXI CCAn_nnnn'nTGG Cuts at: 0 1641 2028 2196 2256 Size: 1641 387 168 60 Fragments arranged by size: 1641 387 168 60 BstYI r'GATC_y Cuts at: 0 648 2036 2256 Size: 648 1388 220 Fragments arranged by size: 1388 648 220 BstZ17I GTA'TAC Cuts at: 0 477 2256 Size: 477 1779 Bsu36I CC'TnA_GG Cuts at: 0 527 2256 Size: 527 1729 BtgI C'CryG_G Cuts at: 0 738 2021 2256 Size: 738 1283 235 Fragments arranged by size: 1283 738 235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 972 1007 2105 2140 2256 Size: 972 35 1098 35 116 Fragments arranged by size: 1098 972 116 35 35 EarI CTCTTCn'nnn_ Cuts at: 0 229 2047 2206 2233 2256 Size: 229 1818 159 27 23 Fragments arranged by size: 1818 229 159 27 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1116 2256 Size: 1116 1140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 14 34 208 219 425 502 532 559 Size: 14 20 174 11 206 77 30 27 Cuts at: 559 661 1286 1313 2071 2256 Size: 102 625 27 758 185 Fragments arranged by size: 758 625 206 185 174 102 77 30 27 27 20 14 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1301 2256 Size: 1301 955 EcoO109I rG'GnC_Cy Cuts at: 0 1294 1602 2200 2256 Size: 1294 308 598 56 Fragments arranged by size: 1294 598 308 56 EcoRI G'AATT_C Cuts at: 0 1232 2256 Size: 1232 1024 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1419 2235 2256 Size: 1419 816 21 Fragments arranged by size: 1419 816 21 HaeII r_GCGC'y Cuts at: 0 2188 2256 Size: 2188 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 358 390 778 810 1040 1072 2187 2219 Size: 358 32 388 32 230 32 1115 32 Cuts at: 2219 2256 Size: 37 Fragments arranged by size: 1115 388 358 230 37 32 32 32 32 HincII GTy'rAC Cuts at: 0 612 2256 Size: 612 1644 KpnI G_GTAC'C Cuts at: 0 1178 2256 Size: 1178 1078 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1775 2256 Size: 1775 481 MslI CAynn'nnrTG Cuts at: 0 874 1639 2026 2194 2256 Size: 874 765 387 168 62 Fragments arranged by size: 874 765 387 168 62 MspA1I CmG'CkG Cuts at: 0 403 1472 1553 2256 Size: 403 1069 81 703 Fragments arranged by size: 1069 703 403 81 NcoI C'CATG_G Cuts at: 0 738 2021 2256 Size: 738 1283 235 Fragments arranged by size: 1283 738 235 NdeI CA'TA_TG Cuts at: 0 1075 2256 Size: 1075 1181 NspI r_CATG'y Cuts at: 0 1872 2256 Size: 1872 384 PflMI CCAn_nnn'nTGG Cuts at: 0 639 1889 2256 Size: 639 1250 367 Fragments arranged by size: 1250 639 367 PfoI T'CCnGG_A Cuts at: 0 581 1835 1922 2256 Size: 581 1254 87 334 Fragments arranged by size: 1254 581 334 87 PpuMI rG'GwC_Cy Cuts at: 0 1602 2256 Size: 1602 654 PvuII CAG'CTG Cuts at: 0 403 2256 Size: 403 1853 SapI GCTCTTCn'nnn_ Cuts at: 0 2206 2233 2256 Size: 2206 27 23 Fragments arranged by size: 2206 27 23 SexAI A'CCwGG_T Cuts at: 0 1598 2256 Size: 1598 658 SfcI C'TryA_G Cuts at: 0 724 2256 Size: 724 1532 SmaI CCC'GGG Cuts at: 0 1090 1172 2256 Size: 1090 82 1084 Fragments arranged by size: 1090 1084 82 SspI AAT'ATT Cuts at: 0 58 67 2256 Size: 58 9 2189 Fragments arranged by size: 2189 58 9 StuI AGG'CCT Cuts at: 0 355 525 666 896 2256 Size: 355 170 141 230 1360 Fragments arranged by size: 1360 355 230 170 141 StyI C'CwwG_G Cuts at: 0 738 797 1130 1535 2021 2256 Size: 738 59 333 405 486 235 Fragments arranged by size: 738 486 405 333 235 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 437 2256 Size: 437 1819 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 478 510 1649 1681 2256 Size: 478 32 1139 32 575 Fragments arranged by size: 1139 575 478 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1600 2256 Size: 1600 656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 762 1581 2256 Size: 762 819 675 Fragments arranged by size: 819 762 675 XmaI C'CCGG_G Cuts at: 0 1088 1170 2256 Size: 1088 82 1086 Fragments arranged by size: 1088 1086 82 XmnI GAAnn'nnTTC Cuts at: 0 832 1232 1315 2256 Size: 832 400 83 941 Fragments arranged by size: 941 832 400 83 Enzymes that do cut: AccI Acc65I AclI AcuI AlfI AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI CspCI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MmeI MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI PvuII SapI SexAI SfcI SmaI SspI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BspEI BsrBI BsrGI BssHII BstBI BstEII BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SwaI TatI XbaI XhoI ZraI