################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 111 CGCG CG^CG AccIII 229 TCCGGA T^CCGGA AlwI 9 GATCC GGATC(4/5) AvaI 55 CCCGAG C^[CT]CG[GA]G BanII 60 GGGCTC G[GA]GC[CT]^C BbsI 25 GAAGAC GAAGAC(2/6) BbvI 80 GCTGC GCAGC(8/12) BmyI 60 GGGCTC G[AGT]GC[ACT]^C BpuAI 25 GAAGAC GAAGAC(2/6) BsaJI 56 CCGAGG C^C[ACGT][ACGT]GG BseAI 229 TCCGGA T^CCGGA BsiEI 153 CGGCCG CG[GA][CT]^CG BsoBI 55 CCCGAG C^[CT]CG[GA]G Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C BspEI 229 TCCGGA T^CCGGA BssKI 120 CCAGG ^CC[ACGT]GG BstNI 120 CCAGG CC^[AT]GG BstUI 111 CGCG CG^CG BstXI 161 CCAAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 8 AGATCC [GA]^GATC[CT] CfoI 47 GCGC GCG^C EaeI 153 CGGCCG [CT]^GGCC[GA] EagI 153 CGGCCG C^GGCCG EclXI 153 CGGCCG C^GGCCG Eco52I 153 CGGCCG C^GGCCG EcoRII 120 CCAGG ^CC[AT]GG FauI 112 GCGGG CCCGC(4/6) HhaI 47 GCGC GCG^C HinP1I 47 GCGC G^CGC HphI 171 GGTGA GGTGA(8/7) MaeII 197 ACGT A^CGT MflI 8 AGATCC [GA]^GATC[CT] MroI 229 TCCGGA T^CCGGA MspA1I 78 CAGCTG C[AC]G^C[GT]G MvaI 120 CCAGG CC^[AT]GG MvnI 111 CGCG CG^CG NlaIV 61 GGCTCC GG[ACGT]^[ACGT]CC PstI 88 CTGCAG CTGCA^G PvuII 78 CAGCTG CAG^CTG SbfI 87 CCTGCAGG CCTGCA^GG ScrFI 120 CCAGG CC^[ACGT]GG SfiI 145 GGCCAGATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 87 CCTGCAGG CCTGCA^GG TaqI 176 TCGA T^CGA TseI 80 GCTGC G^C[AT]GC TthHB8I 176 TCGA T^CGA XhoII 8 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 79 AGCT AG^CT 140 AGCT BglI 38 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 146 GCCAGATCGGC BsaWI 185 ACCGGA [AT]^CCGG[AT] 229 TCCGGA BstF5I 180 GGATG GGATG(2/0) 209 GGATG Cac8I 86 GCCTGC GC[ACGT]^[ACGT]GC 112 GCGGGC Cfr13I 36 GGGCC G^G[ACGT]CC 144 GGGCC FokI 180 GGATG GGATG(9/13) 209 GGATG HapII 186 CCGG C^CGG 230 CCGG HpaII 186 CCGG C^CGG 230 CCGG MboII 25 GAAGA GAAGA(8/7) 107 TCTTC MspI 186 CCGG C^CGG 230 CCGG MwoI 38 GCCGCTTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 146 GCCAGATCGGC Sau96I 36 GGGCC G^G[ACGT]CC 144 GGGCC SfcI 18 CTACAG C^T[GA][CT]AG 88 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AciI BsiYI BslI DpnII Fnu4HI HaeIII ItaI MboI MnlI NdeII Sau3AI