(Linear) (Six-base) MAPSORT of: KIEE5086.seq Check: 6301 from: 1 to: 237 KIEE5086.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 223 237 Size: 60 163 14 Fragments arranged by size: 163 60 14 AvaI C'yCGr_G Cuts at: 0 55 237 Size: 55 182 BanII G_rGCy'C Cuts at: 0 64 237 Size: 64 173 BbsI GAAGACnn'nnnn_ Cuts at: 0 32 237 Size: 32 205 BglI GCCn_nnn'nGGC Cuts at: 0 44 152 237 Size: 44 108 85 Fragments arranged by size: 108 85 44 BmgBI CAC'GTC Cuts at: 0 198 237 Size: 198 39 BsaWI w'CCGG_w Cuts at: 0 185 229 237 Size: 185 44 8 Fragments arranged by size: 185 44 8 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 237 Size: 85 30 122 Fragments arranged by size: 122 85 30 BseYI C'CCAG_C Cuts at: 0 141 237 Size: 141 96 BsiEI CG_ry'CG Cuts at: 0 156 237 Size: 156 81 Bsp1286I G_dGCh'C Cuts at: 0 64 237 Size: 64 173 BspEI T'CCGG_A Cuts at: 0 229 237 Size: 229 8 BstXI CCAn_nnnn'nTGG Cuts at: 0 168 237 Size: 168 69 BstYI r'GATC_y Cuts at: 0 8 237 Size: 8 229 EaeI y'GGCC_r Cuts at: 0 153 237 Size: 153 84 EagI C'GGCC_G Cuts at: 0 153 237 Size: 153 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 223 237 Size: 60 163 14 Fragments arranged by size: 163 60 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 85 117 158 190 237 Size: 85 32 41 32 47 Fragments arranged by size: 85 47 41 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 237 Size: 89 148 MspA1I CmG'CkG Cuts at: 0 80 237 Size: 80 157 PstI C_TGCA'G Cuts at: 0 92 237 Size: 92 145 PvuII CAG'CTG Cuts at: 0 80 237 Size: 80 157 SbfI CC_TGCA'GG Cuts at: 0 92 237 Size: 92 145 SfcI C'TryA_G Cuts at: 0 18 88 237 Size: 18 70 149 Fragments arranged by size: 149 70 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 152 237 Size: 152 85 Enzymes that do cut: AcuI AvaI BanII BbsI BglI BmgBI BsaWI BsaXI BseYI BsiEI Bsp1286I BspEI BstXI BstYI EaeI EagI Eco57MI Hin4I MmeI MspA1I PstI PvuII SbfI SfcI SfiI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI