################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BbeI BciVI BclI BfrI BglII BlpI Bpu1102I BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MflI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 928 GTCTAC GT^[AC][GT]AC AccII 1260 CGCG CG^CG AciI 1281 CCGC CCGC(-3/-1) AcyI 405 GACGCC G[GA]^CG[CT]C AfaI 527 GTAC GT^AC AflIII 608 ACACGT A^C[GA][CT]GT AlwI 399 GGATC GGATC(4/5) AseI 298 ATTAAT AT^TAAT AsnI 298 ATTAAT AT^TAAT AspHI 646 GAGCTC G[AT]GC[AT]^C AvaII 1180 GGACC G^G[AT]CC AvrII 1030 CCTAGG C^CTAGG BbrPI 1000 CACGTG CAC^GTG BbvCI 798 CCTCAGC CCTCAGC(-5/-2) BcgI 825 CGAGAATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1153 CCGGG CC^[GC]GG BfaI 1031 CTAG C^TAG BlnI 1030 CCTAGG C^CTAGG BsaAI 1000 CACGTG [CT]AC^GT[GA] BsaBI 394 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 405 GACGCC G[GA]^CG[CT]C BsaWI 412 TCCGGT [AT]^CCGG[AT] BsiHKAI 646 GAGCTC G[AT]GC[AT]^C BsmBI 611 CGTCTC CGTCTC(1/5) BspHI 881 TCATGA T^CATGA BstEII 471 GGTGACC G^GT[ACGT]ACC BstPI 471 GGTGACC G^GT[ACGT]ACC BstUI 1260 CGCG CG^CG DrdI 121 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 471 GGTGACC G^GT[ACGT]ACC Hin1I 405 GACGCC G[GA]^CG[CT]C MaeI 1031 CTAG C^TAG MamI 394 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 90 CAATTG C^AATTG MspA1I 247 CAGCTG C[AC]G^C[GT]G MunI 90 CAATTG C^AATTG MvnI 1260 CGCG CG^CG NciI 1153 CCGGG CC^[GC]GG NspI 566 GCATGC [GA]CATG^[CT] PmaCI 1000 CACGTG CAC^GTG PmlI 1000 CACGTG CAC^GTG PpuMI 1179 GGGACCC [GA]G^G[AT]CC[CT] PshBI 298 ATTAAT AT^TAAT PvuII 247 CAGCTG CAG^CTG RcaI 881 TCATGA T^CATGA RsaI 527 GTAC GT^AC SacI 646 GAGCTC GAGCT^C SfcI 177 CTACAG C^T[GA][CT]AG SphI 566 GCATGC GCATG^C StuI 155 AGGCCT AGG^CCT TaqI 444 TCGA T^CGA Tsp45I 472 GTGAC ^GT[GC]AC TthHB8I 444 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AlwNI 247 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 477 CAGCATCTG BalI 722 TGGCCA TGG^CCA 1004 TGGCCA BanI 765 GGTGCC G^G[CT][GA]CC 910 GGCACC BanII 646 GAGCTC G[GA]GC[CT]^C 1206 GGGCTC BglI 15 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1029 GCCTAGGTGGC BpmI 244 CTCCAG CTGGAG(16/14) 970 CTGGAG BseRI 34 CTCCTC GAGGAG(10/8) 1305 CTCCTC BsrI 770 CCAGT ACTGG(1/-1) 905 CCAGT BstXI 389 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCACCTGCCTGG CfoI 732 GCGC GCG^C 1090 GCGC DraII 1109 AGGGCCT [GA]G^G[ACGT]CC[CT] 1179 GGGACCC DsaI 389 CCATGG C^C[GA][CT]GG 683 CCATGG EaeI 722 TGGCCA [CT]^GGCC[GA] 1004 TGGCCA EcoO109I 1109 AGGGCCT [GA]G^G[ACGT]CC[CT] 1179 GGGACCC HapII 413 CCGG C^CGG 1153 CCGG HgaI 76 GACGC GACGC(5/10) 405 GACGC HhaI 732 GCGC GCG^C 1090 GCGC HinP1I 732 GCGC G^CGC 1090 GCGC HincII 118 GTTGAC GT[CT]^[GA]AC 196 GTCAAC HindII 118 GTTGAC GT[CT]^[GA]AC 196 GTCAAC HinfI 385 GAATC G^A[ACGT]TC 828 GAATC HpaII 413 CCGG C^CGG 1153 CCGG MaeIII 472 GTGAC ^GT[ACGT]AC 485 GTAAC MluNI 722 TGGCCA TGG^CCA 1004 TGGCCA MscI 722 TGGCCA TGG^CCA 1004 TGGCCA MseI 299 TTAA T^TAA 449 TTAA MslI 260 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 390 CATGGATGTG MspI 413 CCGG C^CGG 1153 CCGG NcoI 389 CCATGG C^CATGG 683 CCATGG PflMI 1227 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCACGCGATGG SapI 1275 GCTCTTC GCTCTTC(1/4) 1293 GCTCTTC TfiI 385 GAATC G^A[AT]TC 828 GAATC Tru9I 299 TTAA T^TAA 449 TTAA Van91I 1227 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCACGCGATGG XcmI 221 CCATCCTGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 770 CCAGTAGCTTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AluI BbsI BbvI BmyI BpuAI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI