Restriction map for
pFN21AE5577
(Circular) (Six-base) MAPSORT of: pFN21AE5577.seq Check: 1698 from: 1 to: 6046
pFN21AE5577 6046 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3147 3147
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2934 3389 1937
Size: 997 455 4594
Fragments arranged by size:
4594 997 455
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2527 4109 5493 5761 1632
Size: 895 1582 1384 268 1917
Fragments arranged by size:
1917 1582 1384 895 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 2613 4945 5922 1203
Size: 1410 2332 977 1327
Fragments arranged by size:
2332 1410 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2257 2487 3789 5361 2257
Size: 230 1302 1572 2942
Fragments arranged by size:
2942 1572 1302 230
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 3355 3581 3733 5842 1493
Size: 1862 226 152 2109 1697
Fragments arranged by size:
2109 1862 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2304 4600 160
Size: 872 1272 2296 1606
Fragments arranged by size:
2296 1606 1272 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 3371 1870
Size: 73 1428 4545
Fragments arranged by size:
4545 1428 73
AvrII C'CTAG_G
Cuts at: 3035 3035
Size: 6046
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2770 2915 3367 4822 618
Size: 325 217 627 983 145 452 1455 1842
Fragments arranged by size:
1842 1455 983 627 452 325 217 145
BanII G_rGCy'C
Cuts at: 1391 2655 3215 3365 1391
Size: 1264 560 150 4072
Fragments arranged by size:
4072 1264 560 150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2051 2128 2869 2977 928
Size: 971 152 77 741 108 3997
Fragments arranged by size:
3997 971 741 152 108 77
BbvCI CC'TCA_GC
Cuts at: 2804 2804
Size: 6046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2819 2853 4237 4271 1903
Size: 34 882 34 1384 34 3678
Fragments arranged by size:
3678 1384 882 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 3040 4657
Size: 107 122 71 631 605 354 1014 1617
Cuts at: 4657 136
Size: 1525
Fragments arranged by size:
1617 1525 1014 631 605 354 122 107
71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2775 2842 4107 5263 1790
Size: 248 737 67 1265 1156 2573
Fragments arranged by size:
2573 1265 1156 737 248 67
BmgBI CAC'GTC
Cuts at: 1318 2616 1318
Size: 1298 4748
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2769 3777 4735
Size: 416 53 83 399 24 1352 1008 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1352 1008 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2996 4706 1239
Size: 995 762 1710 2579
Fragments arranged by size:
2579 1710 995 762
Bpu10I CC'TnA_GC
Cuts at: 2101 2804 3214 2101
Size: 703 410 4933
Fragments arranged by size:
4933 703 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2426 2972 3128 3188 4709 882
Size: 617 927 546 156 60 1521 2219
Fragments arranged by size:
2219 1521 927 617 546 156 60
BsaAI yAC'GTr
Cuts at: 493 1204 3007 3837 493
Size: 711 1803 830 2702
Fragments arranged by size:
2702 1803 830 711
BsaBI GATnn'nnATC
Cuts at: 2403 6040 2403
Size: 3637 2409
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2411 3853 4235 4870
Size: 53 83 186 936 878 1442 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1451 1442 936 878 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2417 4479 4877 5151 5298 5709 2417
Size: 2062 398 274 147 411 2754
Fragments arranged by size:
2754 2062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3070 3100 1178
Size: 30 165 30 1667 30 4124
Fragments arranged by size:
4124 1667 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 3301 1186
Size: 844 1271 3931
Fragments arranged by size:
3931 1271 844
BseYI C'CCAG_C
Cuts at: 2578 2863 3018 3181 3231 5249 5745 2578
Size: 285 155 163 50 2018 496 2879
Fragments arranged by size:
2879 2018 496 285 163 155 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2987 3049 3272 1163
Size: 573 1251 62 223 3937
Fragments arranged by size:
3937 1251 573 223 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 4107 4192 5263 2655
Size: 1452 85 1071 3438
Fragments arranged by size:
3438 1452 1071 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
BsmBI CGTCTCn'nnnn_
Cuts at: 2622 2622
Size: 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2655 2775 2842 3215 3365 4107
Size: 399 248 617 120 67 373 150 742
Cuts at: 4107 4192 5263 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 399 373 248 150
120 85 67
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 2886 3935 5665 2886
Size: 1049 1730 3267
Fragments arranged by size:
3267 1730 1049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 2823 3006 3147 3303 3401 844
Size: 1928 51 183 141 156 98 3489
Fragments arranged by size:
3489 1928 183 156 141 98 51
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 2476 1184
Size: 1292 4754
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 3143 2401
Size: 742 5304
BstYI r'GATC_y
Cuts at: 1972 3376 3743 4128 4145 5597 5683 5695 6041
Size: 1404 367 385 17 1452 86 12 346
Cuts at: 6041 1972
Size: 1977
Fragments arranged by size:
1977 1452 1404 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2394 2688 513
Size: 552 939 390 294 3871
Fragments arranged by size:
3871 939 552 390 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3281 487
Size: 683 56 2055 3252
Fragments arranged by size:
3252 2055 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 2424 2446 3061 3090 3562 4356 4376 2151
Size: 273 22 615 29 472 794 20 3821
Fragments arranged by size:
3821 794 615 472 273 29 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 5053 809
Size: 507 816 2921 1802
Fragments arranged by size:
2921 1802 816 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2727 3009 3493
Size: 54 1425 50 275 18 897 282 484
Cuts at: 3493 4382 6011 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 897 889 484 282 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 3287 3305 3976 1353
Size: 715 1219 18 671 3423
Fragments arranged by size:
3423 1219 715 671 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3275 4633 5007 5153 387
Size: 1064 138 1686 1358 374 146 1280
Fragments arranged by size:
1686 1358 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2653
Size: 6046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2527 2996 4109 4706 5493 5761
Size: 393 602 293 469 1113 597 787 268
Cuts at: 5761 1239
Size: 1524
Fragments arranged by size:
1524 1113 787 602 597 469 393 293
268
EcoO109I rG'GnC_Cy
Cuts at: 3115 3185 3115
Size: 70 5976
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2818 2850 3070 3102 3197
Size: 32 135 32 1246 32 220 32 95
Cuts at: 3197 3229 4687 4719 4761 4793 1373
Size: 32 1458 32 42 32 2626
Fragments arranged by size:
2626 1458 1246 220 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2203 3390 3636 669
Size: 988 281 187 78 1187 246 3079
Fragments arranged by size:
3079 1187 988 281 246 187 78
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 2095 3645 2095
Size: 1550 4496
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2729 3011 10
Size: 54 1425 343 897 282 3045
Fragments arranged by size:
3045 1425 897 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2269 2399 4004 4363 4522 518
Size: 648 1103 130 1605 359 159 2042
Fragments arranged by size:
2042 1605 1103 648 359 159 130
MspA1I CmG'CkG
Cuts at: 1606 2254 3462 4139 5287 5532 1606
Size: 648 1208 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 677 648 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2394 2688 513
Size: 552 939 390 294 3871
Fragments arranged by size:
3871 939 552 390 294
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2575 3404 4949 1132
Size: 97 1346 829 1545 2229
Fragments arranged by size:
2229 1545 1346 829 97
PasI CC'CwG_GG
Cuts at: 1348 2638 3074 1348
Size: 1290 436 4320
Fragments arranged by size:
4320 1290 436
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3238 3268 1108
Size: 2130 30 3886
Fragments arranged by size:
3886 2130 30
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PmlI CAC'GTG
Cuts at: 3007 3007
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3185 3185
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PstI C_TGCA'G
Cuts at: 830 3398 830
Size: 2568 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2254 2254
Size: 6046
SacI G_AGCT'C
Cuts at: 2655 2655
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SanDI GG'GwC_CC
Cuts at: 3185 3185
Size: 6046
SapI GCTCTTCn'nnn_
Cuts at: 3287 3305 3287
Size: 18 6028
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2182 3394 4529 5210 5401 826
Size: 219 1137 1212 1135 681 191 1471
Fragments arranged by size:
1471 1212 1137 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 2575 3404 1132
Size: 1443 829 3774
Fragments arranged by size:
3774 1443 829
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 1517
Size: 645 5401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 2688 3035 3485 513
Size: 552 939 390 294 347 450 3074
Fragments arranged by size:
3074 939 552 450 390 347 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 3043 3149 3212 3238 4073 4090
Size: 1255 1452 11 106 63 26 835 17
Cuts at: 4090 4243 4428 4873 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1452 1255 835 445 185 153 106
63 26 17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 6006 6038 2000
Size: 32 3974 32 2008
Fragments arranged by size:
3974 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2233 2782 2233
Size: 549 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI