(Linear) (Six-base) MAPSORT of: KIEE5577.seq Check: 8889 from: 1 to: 1341 KIEE5577.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1142 1341 Size: 1142 199 AccI GT'mk_AC Cuts at: 0 929 1341 Size: 929 412 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 1341 Size: 522 819 AflIII A'CryG_T Cuts at: 0 608 1341 Size: 608 733 AlwNI CAG_nnn'CTG Cuts at: 0 252 482 1341 Size: 252 230 859 Fragments arranged by size: 859 252 230 AseI AT'TA_AT Cuts at: 0 299 1341 Size: 299 1042 AvrII C'CTAG_G Cuts at: 0 1030 1341 Size: 1030 311 BanI G'GyrC_C Cuts at: 0 765 910 1341 Size: 765 145 431 Fragments arranged by size: 765 431 145 BanII G_rGCy'C Cuts at: 0 650 1210 1341 Size: 650 560 131 Fragments arranged by size: 650 560 131 BbsI GAAGACnn'nnnn_ Cuts at: 0 46 123 864 972 1341 Size: 46 77 741 108 369 Fragments arranged by size: 741 369 108 77 46 BbvCI CC'TCA_GC Cuts at: 0 799 1341 Size: 799 542 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 814 848 1341 Size: 814 34 493 Fragments arranged by size: 814 493 34 BglI GCCn_nnn'nGGC Cuts at: 0 21 1035 1341 Size: 21 1014 306 Fragments arranged by size: 1014 306 21 Bme1580I G_kGCm'C Cuts at: 0 33 770 837 1341 Size: 33 737 67 504 Fragments arranged by size: 737 504 67 33 BmgBI CAC'GTC Cuts at: 0 611 1341 Size: 611 730 BmrI ACTGGGnnnn_n' Cuts at: 0 764 1341 Size: 764 577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 991 1341 Size: 229 762 350 Fragments arranged by size: 762 350 229 Bpu10I CC'TnA_GC Cuts at: 0 96 799 1209 1341 Size: 96 703 410 132 Fragments arranged by size: 703 410 132 96 BsaI GGTCTCn'nnnn_ Cuts at: 0 421 967 1123 1183 1341 Size: 421 546 156 60 158 Fragments arranged by size: 546 421 158 156 60 BsaAI yAC'GTr Cuts at: 0 1002 1341 Size: 1002 339 BsaBI GATnn'nnATC Cuts at: 0 398 1341 Size: 398 943 BsaHI Gr'CG_yC Cuts at: 0 406 1341 Size: 406 935 BsaWI w'CCGG_w Cuts at: 0 412 1341 Size: 412 929 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1065 1095 1341 Size: 1065 30 246 Fragments arranged by size: 1065 246 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 1296 1341 Size: 25 1271 45 Fragments arranged by size: 1271 45 25 BseYI C'CCAG_C Cuts at: 0 573 858 1013 1176 1226 1341 Size: 573 285 155 163 50 115 Fragments arranged by size: 573 285 163 155 115 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 982 1044 1267 1341 Size: 982 62 223 74 Fragments arranged by size: 982 223 74 62 BsiHKAI G_wGCw'C Cuts at: 0 650 1341 Size: 650 691 BsmBI CGTCTCn'nnnn_ Cuts at: 0 617 1341 Size: 617 724 Bsp1286I G_dGCh'C Cuts at: 0 33 650 770 837 1210 1341 Size: 33 617 120 67 373 131 Fragments arranged by size: 617 373 131 120 67 33 BspHI T'CATG_A Cuts at: 0 881 1341 Size: 881 460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 767 818 1001 1142 1298 1341 Size: 767 51 183 141 156 43 Fragments arranged by size: 767 183 156 141 51 43 BstEII G'GTnAC_C Cuts at: 0 471 1341 Size: 471 870 BstXI CCAn_nnnn'nTGG Cuts at: 0 396 1138 1341 Size: 396 742 203 Fragments arranged by size: 742 396 203 BtgI C'CryG_G Cuts at: 0 389 683 1341 Size: 389 294 658 Fragments arranged by size: 658 389 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1276 1341 Size: 1276 65 BtsI GCAGTG_nn' Cuts at: 0 146 419 441 1056 1085 1341 Size: 146 273 22 615 29 256 Fragments arranged by size: 615 273 256 146 29 22 DrdI GACnn_nn'nnGTC Cuts at: 0 127 1341 Size: 127 1214 EaeI y'GGCC_r Cuts at: 0 722 1004 1341 Size: 722 282 337 Fragments arranged by size: 722 337 282 EarI CTCTTCn'nnn_ Cuts at: 0 63 1282 1300 1341 Size: 63 1219 18 41 Fragments arranged by size: 1219 63 41 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1270 1341 Size: 1270 71 EcoICRI GAG'CTC Cuts at: 0 648 1341 Size: 648 693 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 522 991 1341 Size: 229 293 469 350 Fragments arranged by size: 469 350 293 229 EcoO109I rG'GnC_Cy Cuts at: 0 1110 1180 1341 Size: 1110 70 161 Fragments arranged by size: 1110 161 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 813 845 1065 1097 1192 1224 1341 Size: 813 32 220 32 95 32 117 Fragments arranged by size: 813 220 117 95 32 32 32 HincII GTy'rAC Cuts at: 0 120 198 1341 Size: 120 78 1143 Fragments arranged by size: 1143 120 78 MfeI C'AATT_G Cuts at: 0 90 1341 Size: 90 1251 MscI TGG'CCA Cuts at: 0 724 1006 1341 Size: 724 282 335 Fragments arranged by size: 724 335 282 MslI CAynn'nnrTG Cuts at: 0 264 394 1341 Size: 264 130 947 Fragments arranged by size: 947 264 130 MspA1I CmG'CkG Cuts at: 0 249 1341 Size: 249 1092 NcoI C'CATG_G Cuts at: 0 389 683 1341 Size: 389 294 658 Fragments arranged by size: 658 389 294 NspI r_CATG'y Cuts at: 0 570 1341 Size: 570 771 PasI CC'CwG_GG Cuts at: 0 633 1069 1341 Size: 633 436 272 Fragments arranged by size: 633 436 272 PflMI CCAn_nnn'nTGG Cuts at: 0 1233 1263 1341 Size: 1233 30 78 Fragments arranged by size: 1233 78 30 PmlI CAC'GTG Cuts at: 0 1002 1341 Size: 1002 339 PpuMI rG'GwC_Cy Cuts at: 0 1180 1341 Size: 1180 161 PvuII CAG'CTG Cuts at: 0 249 1341 Size: 249 1092 SacI G_AGCT'C Cuts at: 0 650 1341 Size: 650 691 SanDI GG'GwC_CC Cuts at: 0 1180 1341 Size: 1180 161 SapI GCTCTTCn'nnn_ Cuts at: 0 1282 1300 1341 Size: 1282 18 41 Fragments arranged by size: 1282 41 18 SfcI C'TryA_G Cuts at: 0 177 1341 Size: 177 1164 SphI G_CATG'C Cuts at: 0 570 1341 Size: 570 771 StuI AGG'CCT Cuts at: 0 157 1341 Size: 157 1184 StyI C'CwwG_G Cuts at: 0 389 683 1030 1341 Size: 389 294 347 311 Fragments arranged by size: 389 347 311 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1027 1038 1144 1207 1233 1341 Size: 1027 11 106 63 26 108 Fragments arranged by size: 1027 108 106 63 26 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 1341 Size: 27 1314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 228 777 1341 Size: 228 549 564 Fragments arranged by size: 564 549 228 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI AseI AvrII BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstEII BstXI BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I HincII MfeI MscI MslI MspA1I NcoI NspI PasI PflMI PmlI PpuMI PvuII SacI SanDI SapI SfcI SphI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI