################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AspEI AspHI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KspI MaeII MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1210 GGTACC G^GTACC AcsI 862 GAATTC [GA]^AATT[CT] AocI 730 CCTCAGG CC^T[ACGT]AGG ApoI 862 GAATTC [GA]^AATT[CT] AseI 156 ATTAAT AT^TAAT AsnI 156 ATTAAT AT^TAAT Asp718I 1210 GGTACC G^GTACC AspI 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 193 GGTCC G^G[AT]CC BamHI 55 GGATCC G^GATCC BanII 35 GAGCCC G[GA]GC[CT]^C BbsI 280 GAAGAC GAAGAC(2/6) BbvI 835 GCTGC GCAGC(8/12) BmyI 35 GAGCCC G[AGT]GC[ACT]^C BpuAI 280 GAAGAC GAAGAC(2/6) BsaI 1027 GGTCTC GGTCTC(1/5) BseRI 1115 CTCCTC GAGGAG(10/8) BsmFI 194 GTCCC GGGAC(10/14) Bsp1286I 35 GAGCCC G[AGT]GC[ACT]^C BstXI 700 CCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 730 CCTCAGG CC^T[ACGT]AGG Cfr13I 193 GGTCC G^G[ACGT]CC DsaI 376 CCGTGG C^C[GA][CT]GG EarI 625 CTCTTC CTCTTC(1/4) Eco81I 730 CCTCAGG CC^T[ACGT]AGG EcoRI 862 GAATTC G^AATTC EcoRV 1015 GATATC GAT^ATC EcoT14I 98 CCAAGG C^C[AT][AT]GG EcoT22I 325 ATGCAT ATGCA^T HaeIII 249 GGCC GG^CC HindIII 788 AAGCTT A^AGCTT KpnI 1210 GGTACC GGTAC^C Ksp632I 625 CTCTTC CTCTTC(1/4) MfeI 879 CAATTG C^AATTG MslI 153 CACATTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 879 CAATTG C^AATTG NsiI 325 ATGCAT ATGCA^T NspI 327 GCATGT [GA]CATG^[CT] PflFI 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 971 GAGTC GAGTC(4/5) PshBI 156 ATTAAT AT^TAAT PstI 461 CTGCAG CTGCA^G Sau96I 193 GGTCC G^G[ACGT]CC SexAI 1023 ACCAGGT A^CC[AT]GGT SspI 640 AATATT AAT^ATT StyI 98 CCAAGG C^C[AT][AT]GG TseI 835 GCTGC G^C[AT]GC Tsp45I 985 GTCAC ^GT[GC]AC Tth111I 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 646 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 679 GAAGCTGTTC BanI 372 GGCACC G^G[CT][GA]CC 1210 GGTACC BfaI 386 CTAG C^TAG 1198 CTAG BpmI 698 CTCCAG CTGGAG(16/14) 593 CTGGAG BsiYI 96 CCCCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 586 CCTCTTGCTGG BslI 96 CCCCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 586 CCTCTTGCTGG BsrBI 3 GAGCGG CCGCTC(-3/-3) 898 GAGCGG BssKI 777 CCAGG ^CC[ACGT]GG 1024 CCAGG BstNI 777 CCAGG CC^[AT]GG 1024 CCAGG BstYI 55 GGATCC [GA]^GATC[CT] 694 GGATCT DdeI 731 CTCAG C^T[ACGT]AG 1106 CTCAG EcoRII 777 CCAGG ^CC[AT]GG 1024 CCAGG MaeI 386 CTAG C^TAG 1198 CTAG MaeIII 736 GTTAC ^GT[ACGT]AC 985 GTCAC MflI 55 GGATCC [GA]^GATC[CT] 694 GGATCT MseI 157 TTAA T^TAA 415 TTAA MvaI 777 CCAGG CC^[AT]GG 1024 CCAGG ScrFI 777 CCAGG CC^[ACGT]GG 1024 CCAGG SfaNI 843 GATGC GCATC(5/9) 670 GCATC SfcI 113 CTACAG C^T[GA][CT]AG 461 CTGCAG TfiI 811 GATTC G^A[AT]TC 909 GATTC Tru9I 157 TTAA T^TAA 415 TTAA XhoII 55 GGATCC [GA]^GATC[CT] 694 GGATCT XmnI 646 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 679 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AfaI AluI AlwI BsaJI BsgI BsmAI BsrI BstF5I DpnII Fnu4HI FokI HinfI HphI ItaI MboI MboII MnlI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Tsp509I TspRI