(Circular) (Six-base) MAPSORT of: pFN21AE1901.seq Check: 9096 from: 1 to: 5992 pFN21AE1901 5992 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3215 3313 3215 Size: 98 5894 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4055 5439 5707 1632 Size: 2423 1384 268 1917 Fragments arranged by size: 2423 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 3735 5307 3735 Size: 1572 4420 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 2867 3301 3527 3679 5788 1493 Size: 1374 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 2162 4546 160 Size: 872 1130 2384 1606 Fragments arranged by size: 2384 1606 1130 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 BamHI G'GATC_C Cuts at: 2060 3322 3689 2060 Size: 1262 367 4363 Fragments arranged by size: 4363 1262 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 3215 3313 4768 618 Size: 325 217 627 590 838 98 1455 1842 Fragments arranged by size: 1842 1455 838 627 590 325 217 98 BanII G_rGCy'C Cuts at: 1391 2044 3311 1391 Size: 653 1267 4072 Fragments arranged by size: 4072 1267 653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 4628 Fragments arranged by size: 4628 971 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BfrBI ATG'CAT Cuts at: 2332 2332 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 4053 5209 1790 Size: 2263 1156 2573 Fragments arranged by size: 2573 2263 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 2511 3723 Size: 416 53 83 399 24 882 212 1212 Cuts at: 3723 4681 442 Size: 958 1753 Fragments arranged by size: 1753 1212 958 882 416 399 212 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 2688 4652 1239 Size: 1380 69 1964 2579 Fragments arranged by size: 2579 1964 1380 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3038 4655 882 Size: 617 1539 1617 2219 Fragments arranged by size: 2219 1617 1539 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4181 4816 275 Size: 53 83 186 936 2266 382 635 1451 Fragments arranged by size: 2266 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2031 2049 2061 1178 Size: 30 165 30 616 12 18 12 5109 Fragments arranged by size: 5109 616 165 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3111 1186 Size: 1925 4067 BseYI C'CCAG_C Cuts at: 5195 5691 5195 Size: 496 5496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2641 2826 2919 1163 Size: 573 905 185 93 4236 Fragments arranged by size: 4236 905 573 185 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4053 4138 5209 4053 Size: 85 1071 4836 Fragments arranged by size: 4836 1071 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 3311 4053 4138 5209 1391 Size: 399 254 1267 742 85 1071 2174 Fragments arranged by size: 2174 1267 1071 742 399 254 85 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3347 844 Size: 2503 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2905 3879 1394 Size: 429 187 895 974 3507 Fragments arranged by size: 3507 974 895 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 4052 5064 4052 Size: 1012 4980 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2712 2712 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2060 2699 3322 3689 4074 4091 5543 5629 Size: 88 639 623 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 639 623 385 367 346 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2736 2736 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 2381 513 Size: 552 1316 4124 Fragments arranged by size: 4124 1316 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2535 2624 3049 3275 3508 4302 4322 2535 Size: 89 425 226 233 794 20 4205 Fragments arranged by size: 4205 794 425 233 226 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2983 3018 3212 3247 529 Size: 35 2419 35 194 35 3274 Fragments arranged by size: 3274 2419 194 35 35 35 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957 Size: 54 1425 50 275 18 1609 889 1629 Cuts at: 5957 8 Size: 43 Fragments arranged by size: 1629 1609 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2636 3922 1353 Size: 1283 1286 3423 Fragments arranged by size: 3423 1286 1283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2619 2688 4055 4652 5439 5707 1239 Size: 393 987 69 1367 597 787 268 1524 Fragments arranged by size: 1524 1367 987 787 597 393 268 69 EcoRI G'AATT_C Cuts at: 2867 3301 3679 2867 Size: 434 378 5180 Fragments arranged by size: 5180 434 378 EcoRV GAT'ATC Cuts at: 3022 3022 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 3018 3050 4633 Size: 32 135 32 473 32 941 32 1583 Cuts at: 4633 4665 4707 4739 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1583 941 473 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 2793 748 Size: 2045 3947 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3219 3317 3219 Size: 98 5894 MfeI C'AATT_G Cuts at: 2884 3591 2884 Size: 707 5285 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2017 5106 5290 1305 Size: 712 3089 184 2007 Fragments arranged by size: 3089 2007 712 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 3950 4309 4468 518 Size: 648 996 1788 359 159 2042 Fragments arranged by size: 2042 1788 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3408 4085 5233 5478 1606 Size: 1802 677 1148 245 2120 Fragments arranged by size: 2120 1802 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5440 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NsiI A_TGCA'T Cuts at: 2334 2334 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2336 3350 4895 1132 Size: 97 1107 1014 1545 2229 Fragments arranged by size: 2229 1545 1107 1014 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PstI C_TGCA'G Cuts at: 830 2470 3344 830 Size: 1640 874 3478 Fragments arranged by size: 3478 1640 874 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 3028 3028 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 2118 2466 3340 4475 5156 5347 826 Size: 219 1073 348 874 1135 681 191 1471 Fragments arranged by size: 1471 1135 1073 874 681 348 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4096 4997 5259 820 Size: 197 926 2153 901 262 1553 Fragments arranged by size: 2153 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 2647 3916 5 Size: 47 2595 1269 2081 Fragments arranged by size: 2595 2081 1269 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2103 3431 513 Size: 552 1038 1328 3074 Fragments arranged by size: 3074 1328 1038 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4019 4036 4189 4374 4819 325 Size: 1255 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4238 5950 96 Size: 274 80 33 51 1443 2261 1712 138 Fragments arranged by size: 2261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2527 2559 3256 3288 5952 5984 2527 Size: 32 697 32 2664 32 2535 Fragments arranged by size: 2664 2535 697 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3030 1316 Size: 1714 4278 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 2655 2688 4121 2655 Size: 33 1433 4526 Fragments arranged by size: 4526 1433 33 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI