################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BcgI BcnI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I FauI FokI FseI FspI HaeII HapII HgaI HhaI HinP1I HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI StyI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 189 GACGTC GACGT^C AccI 1148 GTAGAC GT^[AC][GT]AC AcyI 189 GACGTC G[GA]^CG[CT]C AflII 1083 CTTAAG C^TTAAG AlwNI 120 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 406 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 928 GAGCAC G[AT]GC[AT]^C BamHI 771 GGATCC G^GATCC BbvCI 822 CCTCAGC CCTCAGC(-5/-2) BbvI 660 GCAGC GCAGC(8/12) BciVI 9 GTATCC GTATCC(6/5) BfrI 1083 CTTAAG C^TTAAG BglII 1332 AGATCT A^GATCT BmyI 928 GAGCAC G[AGT]GC[ACT]^C BsaHI 189 GACGTC G[GA]^CG[CT]C BsiHKAI 928 GAGCAC G[AT]GC[AT]^C BsmBI 187 GAGACG CGTCTC(1/5) BsmI 1359 GCATTC GAATGC(1/-1) Bsp1286I 928 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 885 TGTACA T^GTACA BsrDI 834 CATTGC GCAATG(2/0) BsrGI 885 TGTACA T^GTACA EcoRI 275 GAATTC G^AATTC EcoT22I 1019 ATGCAT ATGCA^T Fnu4HI 660 GCAGC GC^[ACGT]GC Hin1I 189 GACGTC G[GA]^CG[CT]C HindIII 548 AAGCTT A^AGCTT HpaI 1165 GTTAAC GTT^AAC ItaI 660 GCAGC GC^[ACGT]GC MfeI 416 CAATTG C^AATTG MunI 416 CAATTG C^AATTG NdeI 664 CATATG CA^TATG NsiI 1019 ATGCAT ATGCA^T ScaI 947 AGTACT AGT^ACT SexAI 251 ACCTGGT A^CC[AT]GGT SspBI 885 TGTACA T^GTACA SwaI 1262 ATTTAAAT ATTT^AAAT TaqI 705 TCGA T^CGA TseI 660 GCAGC G^C[AT]GC TthHB8I 705 TCGA T^CGA XmnI 406 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AseI 1029 ATTAAT AT^TAAT 1422 ATTAAT AsnI 1029 ATTAAT AT^TAAT 1422 ATTAAT BbsI 30 GAAGAC GAAGAC(2/6) 988 GAAGAC BclI 108 TGATCA T^GATCA 855 TGATCA BpuAI 30 GAAGAC GAAGAC(2/6) 988 GAAGAC BseRI 894 CTCCTC GAGGAG(10/8) 376 GAGGAG BsrI 260 CCAGT ACTGG(1/-1) 1438 CCAGT BssKI 252 CCTGG ^CC[ACGT]GG 924 CCAGG BstNI 252 CCTGG CC^[AT]GG 924 CCAGG BstYI 771 GGATCC [GA]^GATC[CT] 1332 AGATCT DraI 147 TTTAAA TTT^AAA 1263 TTTAAA EarI 334 GAAGAG CTCTTC(1/4) 766 GAAGAG EcoRII 252 CCTGG ^CC[AT]GG 924 CCAGG FbaI 108 TGATCA T^GATCA 855 TGATCA HincII 358 GTCAAC GT[CT]^[GA]AC 1165 GTTAAC HindII 358 GTCAAC GT[CT]^[GA]AC 1165 GTTAAC Ksp632I 334 GAAGAG CTCTTC(1/4) 766 GAAGAG MaeII 190 ACGT A^CGT 684 ACGT MflI 771 GGATCC [GA]^GATC[CT] 1332 AGATCT MspA1I 933 CAGCTG C[AC]G^C[GT]G 1364 CAGCTG MvaI 252 CCTGG CC^[AT]GG 924 CCAGG MwoI 570 GCAGAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1079 GCCTCTTAAGC NlaIV 255 GGTTCC GG[ACGT]^[ACGT]CC 771 GGATCC PshBI 1029 ATTAAT AT^TAAT 1422 ATTAAT PvuII 933 CAGCTG CAG^CTG 1364 CAGCTG ScrFI 252 CCTGG CC^[ACGT]GG 924 CCAGG SmlI 897 CTCAAG C^T[CT][GA]AG 1083 CTTAAG SphI 432 GCATGC GCATG^C 1017 GCATGC XbaI 279 TCTAGA T^CTAGA 1347 TCTAGA XhoII 771 GGATCC [GA]^GATC[CT] 1332 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BfaI BsmAI Cac8I DdeI DpnII HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NspI PleI RsaI Sau3AI SfcI SspI TfiI Tru9I Tsp45I Tsp509I TspRI