(Circular) (Six-base) MAPSORT of: pFN21AE3687.seq Check: 3038 from: 1 to: 6160 pFN21AE3687 6160 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2198 3970 278 Size: 53 83 186 936 662 1772 2468 Fragments arranged by size: 2468 1772 936 662 186 83 53 AccI GT'mk_AC Cuts at: 1937 3154 3503 1937 Size: 1217 349 4594 Fragments arranged by size: 4594 1217 349 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2430 2699 3306 4223 5607 5875 1632 Size: 798 269 607 917 1384 268 1917 Fragments arranged by size: 1917 1384 917 798 607 269 268 AflII C'TTAA_G Cuts at: 820 1017 3088 820 Size: 197 2071 3892 Fragments arranged by size: 3892 2071 197 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 2940 1166 Size: 1774 4386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2130 3903 5475 2130 Size: 1773 1572 2815 Fragments arranged by size: 2815 1773 1572 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 2103 2213 2280 2535 3201 3271 3345 3469 Size: 610 110 67 255 666 70 74 124 Cuts at: 3469 3695 3847 5956 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 610 255 226 152 124 110 74 70 67 AseI AT'TA_AT Cuts at: 160 1032 3035 3428 4714 160 Size: 872 2003 393 1286 1606 Fragments arranged by size: 2003 1606 1286 872 393 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 3485 1870 Size: 73 1542 4545 Fragments arranged by size: 4545 1542 73 BamHI G'GATC_C Cuts at: 2776 3490 3857 2776 Size: 714 367 5079 Fragments arranged by size: 5079 714 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3481 4936 618 Size: 325 217 627 1694 1455 1842 Fragments arranged by size: 1842 1694 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3479 1391 Size: 2088 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 3000 928 Size: 971 143 958 4088 Fragments arranged by size: 4088 971 958 143 BbvCI CC'TCA_GC Cuts at: 2828 2828 Size: 6160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2025 4052 5262 2025 Size: 2027 1210 2923 Fragments arranged by size: 2923 2027 1210 BclI T'GATC_A Cuts at: 1548 2113 2860 1548 Size: 565 747 4848 Fragments arranged by size: 4848 747 565 BfrBI ATG'CAT Cuts at: 3026 3026 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4771 136 Size: 107 122 71 631 605 3099 1525 Fragments arranged by size: 3099 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3337 6155 3337 Size: 2818 3342 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 4221 5377 1790 Size: 2431 1156 2573 Fragments arranged by size: 2573 2431 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2259 3891 4849 Size: 416 53 83 399 24 842 1632 958 Cuts at: 4849 442 Size: 1753 Fragments arranged by size: 1753 1632 958 842 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4820 1239 Size: 3581 2579 Bpu10I CC'TnA_GC Cuts at: 2828 2828 Size: 6160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2887 4285 5150 5448 2887 Size: 1398 865 298 3599 Fragments arranged by size: 3599 1398 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 882 Size: 617 3324 2219 Fragments arranged by size: 3324 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2195 3967 4349 4984 Size: 53 83 186 936 662 1772 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1772 1451 936 662 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2890 1186 Size: 1210 494 4456 Fragments arranged by size: 4456 1210 494 BseYI C'CCAG_C Cuts at: 2571 5363 5859 2571 Size: 2792 496 2872 Fragments arranged by size: 2872 2792 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5587 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2937 4221 4306 5377 2937 Size: 1284 85 1071 3720 Fragments arranged by size: 3720 1284 1071 85 BsmI GAATG_Cn' Cuts at: 3364 3671 3764 3364 Size: 307 93 5760 Fragments arranged by size: 5760 307 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2186 Size: 6160 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2937 3479 4221 4306 5377 1391 Size: 399 1147 542 742 85 1071 2174 Fragments arranged by size: 2174 1147 1071 742 542 399 85 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3515 844 Size: 2671 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2838 4655 4829 65 Size: 1183 1590 1817 174 1396 Fragments arranged by size: 1817 1590 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4804 4991 1700 Size: 110 2994 187 2869 Fragments arranged by size: 2994 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2890 96 Size: 2794 3366 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstYI r'GATC_y Cuts at: 1972 2776 3337 3490 3857 4242 4259 5711 5797 Size: 804 561 153 367 385 17 1452 86 Cuts at: 5797 5809 6155 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 804 561 385 367 346 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5608 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2403 2611 2914 3676 4470 4490 2292 Size: 111 208 303 762 794 20 3962 Fragments arranged by size: 3962 794 762 303 208 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 2154 3270 3464 2154 Size: 1116 194 4850 Fragments arranged by size: 4850 1116 194 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3607 4496 6125 Size: 54 1425 50 275 18 1777 889 1629 Cuts at: 6125 8 Size: 43 Fragments arranged by size: 1777 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2766 4090 1353 Size: 981 432 1324 3423 Fragments arranged by size: 3423 1324 981 432 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2430 2699 3306 4223 4820 5607 5875 Size: 393 798 269 607 917 597 787 268 Cuts at: 5875 1239 Size: 1524 Fragments arranged by size: 1524 917 798 787 607 597 393 269 268 EcoRI G'AATT_C Cuts at: 2280 3469 3847 2280 Size: 1189 378 4593 Fragments arranged by size: 4593 1189 378 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 5307 1678 Size: 3629 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373 Size: 32 135 32 3229 32 42 32 2626 Fragments arranged by size: 3229 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2365 3172 3504 3750 669 Size: 988 281 427 807 332 246 3079 Fragments arranged by size: 3079 988 807 427 332 281 246 HindIII A'AGCT_T Cuts at: 748 2553 748 Size: 1805 4355 HpaI GTT'AAC Cuts at: 3172 3750 3172 Size: 578 5582 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 2421 3759 2421 Size: 1338 4822 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2856 2940 3006 3165 3216 4118 4477 Size: 648 1690 84 66 159 51 902 359 Cuts at: 4477 4636 518 Size: 159 2042 Fragments arranged by size: 2042 1690 902 648 359 159 159 84 66 51 MspA1I CmG'CkG Cuts at: 1606 2940 3371 3576 4253 5401 5646 1606 Size: 1334 431 205 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 677 431 245 205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5608 NdeI CA'TA_TG Cuts at: 387 2670 387 Size: 2283 3877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NsiI A_TGCA'T Cuts at: 3028 3028 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2441 2759 3026 3030 3182 3518 5063 Size: 97 1212 318 267 4 152 336 1545 Cuts at: 5063 1132 Size: 2229 Fragments arranged by size: 2229 1545 1212 336 318 267 152 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 2928 2928 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 2805 3730 2805 Size: 925 5235 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2904 2936 2904 Size: 32 6128 PstI C_TGCA'G Cuts at: 830 3512 830 Size: 2682 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2940 3371 2940 Size: 431 5729 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 2954 4408 6120 2954 Size: 1454 1712 2994 Fragments arranged by size: 2994 1712 1454 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 6160 SfcI C'TryA_G Cuts at: 826 1045 2304 2391 2427 3508 4643 5324 5515 Size: 219 1259 87 36 1081 1135 681 191 Cuts at: 5515 826 Size: 1471 Fragments arranged by size: 1471 1259 1135 1081 681 219 191 87 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2902 3088 4264 5165 5427 820 Size: 197 926 959 186 1176 901 262 1553 Fragments arranged by size: 1553 1176 959 926 901 262 197 186 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 2441 3026 3518 1132 Size: 1309 585 492 3774 Fragments arranged by size: 3774 1309 585 492 SspI AAT'ATT Cuts at: 5 52 2159 2648 3257 3308 3317 4084 5 Size: 47 2107 489 609 51 9 767 2081 Fragments arranged by size: 2107 2081 767 609 489 51 47 9 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 3599 513 Size: 552 2534 3074 Fragments arranged by size: 3074 2534 552 SwaI ATTT'AAAT Cuts at: 3270 3270 Size: 6160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2864 4187 4204 4357 4542 4987 325 Size: 1255 1284 1323 17 153 185 445 1498 Fragments arranged by size: 1498 1323 1284 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2272 2890 2952 Size: 274 80 33 51 1443 295 618 62 Cuts at: 2952 4406 6118 96 Size: 1454 1712 138 Fragments arranged by size: 1712 1454 1443 618 295 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 2284 3352 3496 2284 Size: 1068 144 4948 Fragments arranged by size: 4948 1068 144 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 2415 4289 2415 Size: 1874 4286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2196 3968 276 Size: 53 83 186 936 662 1772 2468 Fragments arranged by size: 2468 1772 936 662 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI XcmI