################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BciVI BcnI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BstEII BstPI ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III EcoO65I EcoRI EcoT22I FspI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 523 GTATAC GT^[AC][GT]AC AocI 988 CCTGAGG CC^T[ACGT]AGG BbvCI 1171 GCTGAGG CCTCAGC(-5/-2) BcgI 1971 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1199 TGATCA T^GATCA BfaI 670 CTAG C^TAG BglI 1994 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1390 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1390 GCTGAGC GC^T[ACGT]AGC BsaI 1915 GAGACC GGTCTC(1/5) BsiEI 1408 CGGCCG CG[GA][CT]^CG BsmFI 165 GTCCC GGGAC(10/14) BsrFI 1406 GCCGGC [GA]^CCGG[CT] Bst1107I 523 GTATAC GTA^TAC BstBI 740 TTCGAA TT^CGAA BstXI 1145 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1554 AGATCC [GA]^GATC[CT] BstZ17I 523 GTATAC GTA^TAC Bsu36I 988 CCTGAGG CC^T[ACGT]AGG CelII 1390 GCTGAGC GC^T[ACGT]AGC Cfr10I 1406 GCCGGC [GA]^CCGG[CT] EaeI 1408 CGGCCG [CT]^GGCC[GA] EagI 1408 CGGCCG C^GGCCG EclXI 1408 CGGCCG C^GGCCG Eco52I 1408 CGGCCG C^GGCCG Eco81I 988 CCTGAGG CC^T[ACGT]AGG EcoNI 1026 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1465 GATATC GAT^ATC EcoT14I 1497 CCAAGG C^C[AT][AT]GG FauI 1281 GCGGG CCCGC(4/6) FbaI 1199 TGATCA T^GATCA FseI 1405 GGCCGGCC GGCCGG^CC HaeII 138 GGCGCT [GA]GCGC^[CT] HindIII 1295 AAGCTT A^AGCTT MaeI 670 CTAG C^TAG MflI 1554 AGATCC [GA]^GATC[CT] NaeI 1406 GCCGGC GCC^GGC NgoMIV 1406 GCCGGC G^CCGGC NspV 740 TTCGAA TT^CGAA PpuMI 1587 AGGACCC [GA]G^G[AT]CC[CT] SfuI 740 TTCGAA TT^CGAA StyI 1497 CCAAGG C^C[AT][AT]GG XhoII 1554 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Alw44I 335 GTGCAC G^TGCAC 665 GTGCAC ApaLI 335 GTGCAC G^TGCAC 665 GTGCAC Asp700I 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAGCAGTTC AvaI 827 CCCGAG C^[CT]CG[GA]G 1592 CCCGAG AvaII 1485 GGACC G^G[AT]CC 1588 GGACC BsoBI 827 CCCGAG C^[CT]CG[GA]G 1592 CCCGAG BssSI 373 CACGAG CACGAG(-5/-1) 1577 CTCGTG BstAPI 667 GCACTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1308 GCAGAGGCTGC BstF5I 11 GGATG GGATG(2/0) 1677 GGATG DraII 1022 AGGCCCT [GA]G^G[ACGT]CC[CT] 1587 AGGACCC EcoO109I 1022 AGGCCCT [GA]G^G[ACGT]CC[CT] 1587 AGGACCC FokI 11 GGATG GGATG(9/13) 1677 GGATG HapII 1407 CCGG C^CGG 1431 CCGG HgaI 45 GACGC GACGC(5/10) 111 GACGC HpaII 1407 CCGG C^CGG 1431 CCGG MseI 298 TTAA T^TAA 449 TTAA MspI 1407 CCGG C^CGG 1431 CCGG PleI 684 GACTC GAGTC(4/5) 163 GAGTC Tru9I 298 TTAA T^TAA 449 TTAA XmnI 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAGCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I