(Circular) (Six-base) MAPSORT of: pFN21AE1273.seq Check: 5366 from: 1 to: 6793 pFN21AE1273 6793 bp With 182 enzymes: SgfI * June 21, 2019 12:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3253 3352 3253 Size: 99 6694 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4603 278 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 4136 1937 Size: 592 1607 4594 Fragments arranged by size: 4594 1607 592 Acc65I G'GTAC_C Cuts at: 4114 4114 Size: 6793 AclI AA'CG_TT Cuts at: 1563 4920 5293 1563 Size: 3357 373 3063 Fragments arranged by size: 3357 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 3174 3903 3936 4856 6240 6508 1632 Size: 793 749 729 33 920 1384 268 1917 Fragments arranged by size: 1917 1384 920 793 749 729 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 5692 6669 1203 Size: 4489 977 1327 Fragments arranged by size: 4489 1327 977 AgeI A'CCGG_T Cuts at: 5624 5624 Size: 6793 AhdI GACnn_n'nnGTC Cuts at: 5522 5522 Size: 6793 AleI CACnn'nnGTG Cuts at: 1166 2667 3171 1166 Size: 1501 504 4788 Fragments arranged by size: 4788 1501 504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3189 1184 Size: 2005 4788 AlwNI CAG_nnn'CTG Cuts at: 2053 2891 3250 3283 3319 3617 4064 4536 6108 Size: 838 359 33 36 298 447 472 1572 Cuts at: 6108 2053 Size: 2738 Fragments arranged by size: 2738 1572 838 472 447 359 298 36 33 ApaLI G'TGCA_C Cuts at: 2340 2670 4850 6006 2340 Size: 330 2180 1156 3127 Fragments arranged by size: 3127 2180 1156 330 ApoI r'AATT_y Cuts at: 1493 2617 2942 3209 3686 3718 4102 4328 4480 Size: 1124 325 267 477 32 384 226 152 Cuts at: 4480 6589 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1124 477 384 325 267 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 5347 160 Size: 872 4315 1606 Fragments arranged by size: 4315 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3597 4118 1870 Size: 73 889 765 521 4545 Fragments arranged by size: 4545 889 765 521 73 BamHI G'GATC_C Cuts at: 4123 4490 4123 Size: 367 6426 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4114 5569 618 Size: 325 217 627 2327 1455 1842 Fragments arranged by size: 2327 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4112 1391 Size: 2721 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2442 2664 2910 4053 928 Size: 971 543 222 246 1143 3668 Fragments arranged by size: 3668 1143 971 543 246 222 BbvCI CC'TCA_GC Cuts at: 3177 3177 Size: 6793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3965 3999 4984 5018 1903 Size: 34 2028 34 985 34 3678 Fragments arranged by size: 3678 2028 985 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4685 5895 4685 Size: 1210 5583 BclI T'GATC_A Cuts at: 1548 3204 1548 Size: 1656 5137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4005 5404 136 Size: 107 122 71 631 605 2333 1399 1525 Fragments arranged by size: 2333 1525 1399 631 605 122 107 71 BglII A'GATC_T Cuts at: 6788 6788 Size: 6793 BlpI GC'TnA_GC Cuts at: 3396 4210 3396 Size: 814 5979 Bme1580I G_kGCm'C Cuts at: 1790 2344 2674 3168 3330 4854 6010 1790 Size: 554 330 494 162 1524 1156 2573 Fragments arranged by size: 2573 1524 1156 554 494 330 162 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6793 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 4524 5482 Size: 416 53 83 399 24 759 2348 958 Cuts at: 5482 442 Size: 1753 Fragments arranged by size: 2348 1753 958 759 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 6793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 3053 3923 5453 1239 Size: 1229 585 870 1530 2579 Fragments arranged by size: 2579 1530 1229 870 585 Bpu10I CC'TnA_GC Cuts at: 3177 3177 Size: 6793 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 2676 3095 3422 4918 5783 6081 2132 Size: 544 419 327 1496 865 298 2844 Fragments arranged by size: 2844 1496 865 544 419 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3914 5456 882 Size: 617 2415 1542 2219 Fragments arranged by size: 2415 2219 1542 617 BsaAI yAC'GTr Cuts at: 493 1204 4584 493 Size: 711 3380 2702 Fragments arranged by size: 3380 2702 711 BsaBI GATnn'nnATC Cuts at: 6787 6787 Size: 6793 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4600 4982 5617 275 Size: 53 83 186 936 3067 382 635 1451 Fragments arranged by size: 3067 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5226 5624 5898 6045 6456 5226 Size: 398 274 147 411 5563 Fragments arranged by size: 5563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6568 Fragments arranged by size: 6568 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3011 3029 3050 3092 3116 3284 3419 Size: 1210 615 18 21 42 24 168 135 Cuts at: 3419 3875 4040 4052 1186 Size: 456 165 12 3927 Fragments arranged by size: 3927 1210 615 456 168 165 135 42 24 21 18 12 BseYI C'CCAG_C Cuts at: 3276 3652 5996 6492 3276 Size: 376 2344 496 3577 Fragments arranged by size: 3577 2344 496 376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2667 2861 3278 1163 Size: 573 931 194 417 4678 Fragments arranged by size: 4678 931 573 417 194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3416 4243 5004 6032 1540 Size: 275 186 1415 827 761 1028 2301 Fragments arranged by size: 2301 1415 1028 827 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2344 2674 3021 4854 4939 6010 2024 Size: 320 330 347 1833 85 1071 2807 Fragments arranged by size: 2807 1833 1071 347 330 320 85 BsmI GAATG_Cn' Cuts at: 4304 4397 4304 Size: 93 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2344 2674 3021 3168 3330 4112 Size: 399 234 320 330 347 147 162 782 Cuts at: 4112 4854 4939 6010 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 399 347 330 320 234 162 147 85 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 4682 6412 4682 Size: 1730 5063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3043 3253 3352 4148 844 Size: 2199 210 99 796 3489 Fragments arranged by size: 3489 2199 796 210 99 BsrBI CCG'CTC Cuts at: 1394 1823 4680 1394 Size: 429 2857 3507 Fragments arranged by size: 3507 2857 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5288 5462 65 Size: 1183 4040 174 1396 Fragments arranged by size: 4040 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3411 5437 5624 1700 Size: 110 1601 2026 187 2869 Fragments arranged by size: 2869 2026 1601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6793 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 2378 3582 4853 5865 2378 Size: 1204 1271 1012 3306 Fragments arranged by size: 3306 1271 1204 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2678 3319 794 Size: 1884 641 4268 Fragments arranged by size: 4268 1884 641 BstBI TT'CG_AA Cuts at: 2746 5597 2746 Size: 2851 3942 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6793 BstXI CCAn_nnnn'nTGG Cuts at: 3157 3157 Size: 6793 BstYI r'GATC_y Cuts at: 1972 3559 4123 4490 4875 4892 6344 6430 6442 Size: 1587 564 367 385 17 1452 86 12 Cuts at: 6442 6788 1972 Size: 346 1977 Fragments arranged by size: 1977 1587 1452 564 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2530 2530 Size: 6793 Bsu36I CC'TnA_GG Cuts at: 2994 2994 Size: 6793 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5302 Fragments arranged by size: 5302 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2108 2405 487 Size: 683 56 882 297 4875 Fragments arranged by size: 4875 882 683 297 56 BtsI GCAGTG_nn' Cuts at: 3261 4309 5103 5123 3261 Size: 1048 794 20 4931 Fragments arranged by size: 4931 1048 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6758 DraI TTT'AAA Cuts at: 4097 4097 Size: 6793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5800 809 Size: 507 4484 1802 Fragments arranged by size: 4484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3413 4240 5129 Size: 54 1425 50 275 18 1583 827 889 Cuts at: 5129 6758 8 Size: 1629 43 Fragments arranged by size: 1629 1583 1425 889 827 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3413 4240 1537 Size: 275 1601 827 4090 Fragments arranged by size: 4090 1601 827 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2538 2890 3006 3732 3843 3852 3879 Size: 1053 132 352 116 726 111 9 27 Cuts at: 3879 3912 3966 4029 4075 4723 1353 Size: 33 54 63 46 648 3423 Fragments arranged by size: 3423 1053 726 648 352 132 116 111 63 54 46 33 27 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2167 5380 5754 5900 387 Size: 1064 138 578 3213 374 146 1280 Fragments arranged by size: 3213 1280 1064 578 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2468 3053 3174 3903 3923 3936 Size: 393 793 43 585 121 729 20 13 Cuts at: 3936 4856 5453 6240 6508 1239 Size: 920 597 787 268 1524 Fragments arranged by size: 1524 920 793 787 729 597 585 393 268 121 43 20 13 EcoNI CCTnn'n_nnAGG Cuts at: 3035 3035 Size: 6793 EcoO109I rG'GnC_Cy Cuts at: 3028 3593 3028 Size: 565 6228 EcoRI G'AATT_C Cuts at: 4102 4480 4102 Size: 378 6415 EcoRV GAT'ATC Cuts at: 3472 3472 Size: 6793 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2258 2288 2258 Size: 30 6763 FseI GG_CCGG'CC Cuts at: 3415 3415 Size: 6793 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 2147 5940 1678 Size: 469 3793 2531 Fragments arranged by size: 3793 2531 469 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3599 3631 5434 5466 5508 Size: 32 135 32 2027 32 1803 32 42 Cuts at: 5508 5540 1373 Size: 32 2626 Fragments arranged by size: 2626 2027 1803 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4137 4383 669 Size: 988 281 2199 246 3079 Fragments arranged by size: 3079 2199 988 281 246 HindIII A'AGCT_T Cuts at: 748 3300 748 Size: 2552 4241 HpaI GTT'AAC Cuts at: 4383 4383 Size: 6793 KpnI G_GTAC'C Cuts at: 4118 4118 Size: 6793 MfeI C'AATT_G Cuts at: 4392 4392 Size: 6793 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 2992 3216 5907 6091 1305 Size: 1072 615 224 2691 184 2007 Fragments arranged by size: 2691 2007 1072 615 224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4971 Fragments arranged by size: 4971 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 3171 3453 3543 4751 5110 5269 Size: 648 1501 504 282 90 1208 359 159 Cuts at: 5269 518 Size: 2042 Fragments arranged by size: 2042 1501 1208 648 504 359 282 159 90 MspA1I CmG'CkG Cuts at: 1606 2122 3283 3635 4209 4886 6034 6279 1606 Size: 516 1161 352 574 677 1148 245 2120 Fragments arranged by size: 2120 1161 1148 677 574 516 352 245 NaeI GCC'GGC Cuts at: 3413 3413 Size: 6793 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5302 Fragments arranged by size: 5302 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6793 NgoMIV G'CCGG_C Cuts at: 3411 3411 Size: 6793 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 4240 4240 Size: 6793 NspI r_CATG'y Cuts at: 1132 1229 4151 5696 1132 Size: 97 2922 1545 2229 Fragments arranged by size: 2922 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6793 PciI A'CATG_T Cuts at: 5692 5692 Size: 6793 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6793 PfoI T'CCnGG_A Cuts at: 2890 3156 2890 Size: 266 6527 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 6375 6407 4862 Size: 32 1481 32 5248 Fragments arranged by size: 5248 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3593 3593 Size: 6793 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6793 PsiI TTA'TAA Cuts at: 2280 4363 2280 Size: 2083 4710 PstI C_TGCA'G Cuts at: 830 2115 3324 3387 4060 4145 830 Size: 1285 1209 63 673 85 3478 Fragments arranged by size: 3478 1285 1209 673 85 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6793 PvuII CAG'CTG Cuts at: 2122 3283 3635 2122 Size: 1161 352 5280 Fragments arranged by size: 5280 1161 352 SalI G'TCGA_C Cuts at: 1936 4135 1936 Size: 2199 4594 SapI GCTCTTCn'nnn_ Cuts at: 2890 3732 3966 4075 2890 Size: 842 234 109 5608 Fragments arranged by size: 5608 842 234 109 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 5041 6753 5041 Size: 1712 5081 SfcI C'TryA_G Cuts at: 826 1045 2111 3320 3383 3636 4056 4141 5276 Size: 219 1066 1209 63 253 420 85 1135 Cuts at: 5276 5957 6148 826 Size: 681 191 1471 Fragments arranged by size: 1471 1209 1135 1066 681 420 253 219 191 85 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6793 SmaI CCC'GGG Cuts at: 1872 4120 1872 Size: 2248 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2691 3074 3401 4897 5798 Size: 197 926 204 544 383 327 1496 901 Cuts at: 5798 6060 820 Size: 262 1553 Fragments arranged by size: 1553 1496 926 901 544 383 327 262 204 197 SnaBI TAC'GTA Cuts at: 493 4584 493 Size: 4091 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6793 SphI G_CATG'C Cuts at: 1132 4151 1132 Size: 3019 3774 SspI AAT'ATT Cuts at: 5 52 4717 5 Size: 47 4665 2081 Fragments arranged by size: 4665 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6793 StyI C'CwwG_G Cuts at: 513 1065 2004 3502 4232 513 Size: 552 939 1498 730 3074 Fragments arranged by size: 3074 1498 939 730 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2942 4820 4837 4990 5175 5620 325 Size: 1255 1362 1878 17 153 185 445 1498 Fragments arranged by size: 1878 1498 1362 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5039 6751 96 Size: 274 80 33 51 1443 3062 1712 138 Fragments arranged by size: 3062 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 2334 2366 3101 3133 6753 6785 2153 Size: 32 149 32 735 32 3620 32 2161 Fragments arranged by size: 3620 2161 735 149 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6793 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6793 XmaI C'CCGG_G Cuts at: 1870 4118 1870 Size: 2248 4545 XmnI GAAnn'nnTTC Cuts at: 2268 2886 4922 2268 Size: 618 2036 4139 Fragments arranged by size: 4139 2036 618 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4601 276 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI