(Linear) (Six-base) MAPSORT of: KIEE1273.seq Check: 8683 from: 1 to: 2088 KIEE1273.orf With 182 enzymes: SgfI * June 21, 2019 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1248 1347 2088 Size: 1248 99 741 Fragments arranged by size: 1248 741 99 AccI GT'mk_AC Cuts at: 0 524 2088 Size: 524 1564 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 1169 1898 1931 2088 Size: 420 749 729 33 157 Fragments arranged by size: 749 729 420 157 33 AleI CACnn'nnGTG Cuts at: 0 662 1166 2088 Size: 662 504 922 Fragments arranged by size: 922 662 504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1184 2088 Size: 1184 904 AlwNI CAG_nnn'CTG Cuts at: 0 48 886 1245 1278 1314 1612 2059 2088 Size: 48 838 359 33 36 298 447 29 Fragments arranged by size: 838 447 359 298 48 36 33 29 ApaLI G'TGCA_C Cuts at: 0 335 665 2088 Size: 335 330 1423 Fragments arranged by size: 1423 335 330 ApoI r'AATT_y Cuts at: 0 612 937 1204 1681 1713 2088 Size: 612 325 267 477 32 375 Fragments arranged by size: 612 477 375 325 267 32 AvaI C'yCGr_G Cuts at: 0 827 1592 2088 Size: 827 765 496 Fragments arranged by size: 827 765 496 BbsI GAAGACnn'nnnn_ Cuts at: 0 437 659 905 2048 2088 Size: 437 222 246 1143 40 Fragments arranged by size: 1143 437 246 222 40 BbvCI CC'TCA_GC Cuts at: 0 1172 2088 Size: 1172 916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1960 1994 2088 Size: 1960 34 94 Fragments arranged by size: 1960 94 34 BclI T'GATC_A Cuts at: 0 1199 2088 Size: 1199 889 BglI GCCn_nnn'nGGC Cuts at: 0 2000 2088 Size: 2000 88 BlpI GC'TnA_GC Cuts at: 0 1391 2088 Size: 1391 697 Bme1580I G_kGCm'C Cuts at: 0 339 669 1163 1325 2088 Size: 339 330 494 162 763 Fragments arranged by size: 763 494 339 330 162 BmrI ACTGGGnnnn_n' Cuts at: 0 171 2088 Size: 171 1917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 186 2088 Size: 186 1902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 1048 1918 2088 Size: 463 585 870 170 Fragments arranged by size: 870 585 463 170 Bpu10I CC'TnA_GC Cuts at: 0 1172 2088 Size: 1172 916 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 671 1090 1417 2088 Size: 127 544 419 327 671 Fragments arranged by size: 671 544 419 327 127 BsaI GGTCTCn'nnnn_ Cuts at: 0 1909 2088 Size: 1909 179 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 391 1006 1024 1045 1087 1111 1279 1414 Size: 391 615 18 21 42 24 168 135 Cuts at: 1414 1870 2035 2047 2088 Size: 456 165 12 41 Fragments arranged by size: 615 456 391 168 165 135 42 41 24 21 18 12 BseYI C'CCAG_C Cuts at: 0 1271 1647 2088 Size: 1271 376 441 Fragments arranged by size: 1271 441 376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 662 856 1273 2088 Size: 662 194 417 815 Fragments arranged by size: 815 662 417 194 BsiEI CG_ry'CG Cuts at: 0 1411 2088 Size: 1411 677 BsiHKAI G_wGCw'C Cuts at: 0 19 339 669 1016 2088 Size: 19 320 330 347 1072 Fragments arranged by size: 1072 347 330 320 19 Bsp1286I G_dGCh'C Cuts at: 0 19 339 669 1016 1163 1325 2088 Size: 19 320 330 347 147 162 763 Fragments arranged by size: 763 347 330 320 162 147 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1038 1248 1347 2088 Size: 1038 210 99 741 Fragments arranged by size: 1038 741 210 99 BsrFI r'CCGG_y Cuts at: 0 1406 2088 Size: 1406 682 BssSI C'ACGA_G Cuts at: 0 373 1577 2088 Size: 373 1204 511 Fragments arranged by size: 1204 511 373 BstAPI GCAn_nnn'nTGC Cuts at: 0 673 1314 2088 Size: 673 641 774 Fragments arranged by size: 774 673 641 BstBI TT'CG_AA Cuts at: 0 741 2088 Size: 741 1347 BstXI CCAn_nnnn'nTGG Cuts at: 0 1152 2088 Size: 1152 936 BstYI r'GATC_y Cuts at: 0 1554 2088 Size: 1554 534 BstZ17I GTA'TAC Cuts at: 0 525 2088 Size: 525 1563 Bsu36I CC'TnA_GG Cuts at: 0 989 2088 Size: 989 1099 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 103 400 2088 Size: 103 297 1688 Fragments arranged by size: 1688 297 103 BtsI GCAGTG_nn' Cuts at: 0 1256 2088 Size: 1256 832 EaeI y'GGCC_r Cuts at: 0 1408 2088 Size: 1408 680 EagI C'GGCC_G Cuts at: 0 1408 2088 Size: 1408 680 EarI CTCTTCn'nnn_ Cuts at: 0 401 533 885 1001 1727 1838 1847 1874 Size: 401 132 352 116 726 111 9 27 Cuts at: 1874 1907 1961 2024 2070 2088 Size: 33 54 63 46 18 Fragments arranged by size: 726 401 352 132 116 111 63 54 46 33 27 18 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 162 2088 Size: 162 1926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 463 1048 1169 1898 1918 1931 2088 Size: 420 43 585 121 729 20 13 157 Fragments arranged by size: 729 585 420 157 121 43 20 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 1030 2088 Size: 1030 1058 EcoO109I rG'GnC_Cy Cuts at: 0 1023 1588 2088 Size: 1023 565 500 Fragments arranged by size: 1023 565 500 EcoRV GAT'ATC Cuts at: 0 1467 2088 Size: 1467 621 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 253 283 2088 Size: 253 30 1805 Fragments arranged by size: 1805 253 30 FseI GG_CCGG'CC Cuts at: 0 1410 2088 Size: 1410 678 HaeII r_GCGC'y Cuts at: 0 142 2088 Size: 142 1946 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1594 1626 2088 Size: 1594 32 462 Fragments arranged by size: 1594 462 32 HindIII A'AGCT_T Cuts at: 0 1295 2088 Size: 1295 793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 372 987 1211 2088 Size: 372 615 224 877 Fragments arranged by size: 877 615 372 224 MslI CAynn'nnrTG Cuts at: 0 662 1166 1448 1538 2088 Size: 662 504 282 90 550 Fragments arranged by size: 662 550 504 282 90 MspA1I CmG'CkG Cuts at: 0 117 1278 1630 2088 Size: 117 1161 352 458 Fragments arranged by size: 1161 458 352 117 NaeI GCC'GGC Cuts at: 0 1408 2088 Size: 1408 680 NgoMIV G'CCGG_C Cuts at: 0 1406 2088 Size: 1406 682 PfoI T'CCnGG_A Cuts at: 0 885 1151 2088 Size: 885 266 937 Fragments arranged by size: 937 885 266 PpuMI rG'GwC_Cy Cuts at: 0 1588 2088 Size: 1588 500 PsiI TTA'TAA Cuts at: 0 275 2088 Size: 275 1813 PstI C_TGCA'G Cuts at: 0 110 1319 1382 2055 2088 Size: 110 1209 63 673 33 Fragments arranged by size: 1209 673 110 63 33 PvuII CAG'CTG Cuts at: 0 117 1278 1630 2088 Size: 117 1161 352 458 Fragments arranged by size: 1161 458 352 117 SapI GCTCTTCn'nnn_ Cuts at: 0 885 1727 1961 2070 2088 Size: 885 842 234 109 18 Fragments arranged by size: 885 842 234 109 18 SfcI C'TryA_G Cuts at: 0 106 1315 1378 1631 2051 2088 Size: 106 1209 63 253 420 37 Fragments arranged by size: 1209 420 253 106 63 37 SmlI C'TyrA_G Cuts at: 0 142 686 1069 1396 2088 Size: 142 544 383 327 692 Fragments arranged by size: 692 544 383 327 142 StyI C'CwwG_G Cuts at: 0 1497 2088 Size: 1497 591 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 937 2088 Size: 937 1151 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 148 180 329 361 1096 1128 2088 Size: 148 32 149 32 735 32 960 Fragments arranged by size: 960 735 149 148 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 263 881 2088 Size: 263 618 1207 Fragments arranged by size: 1207 618 263 Enzymes that do cut: AarI AccI AcuI AleI AlfI AlwNI ApaLI ApoI AvaI BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I HindIII MmeI MslI MspA1I NaeI NgoMIV PfoI PpuMI PsiI PstI PvuII SapI SfcI SmlI StyI TaqII TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BciVI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstEII BtgI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI