################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 78 GTCTAC GT^[AC][GT]AC AciI 25 CCGC CCGC(-3/-1) AfaI 272 GTAC GT^AC AlwNI 283 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG BanII 386 GAGCTC G[GA]GC[CT]^C BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BpmI 383 CTGGAG CTGGAG(16/14) BsaWI 443 ACCGGA [AT]^CCGG[AT] BseRI 545 GAGGAG GAGGAG(10/8) BsmFI 57 GGGAC GGGAC(10/14) Cfr13I 492 GGCCC G^G[ACGT]CC DpnII 153 GATC ^GATC EcoNI 127 CCTACAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeIII 492 GGCC GG^CC HapII 444 CCGG C^CGG HpaII 444 CCGG C^CGG HphI 407 TCACC GGTGA(8/7) MboI 153 GATC ^GATC MslI 408 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 68 CAGCTG C[AC]G^C[GT]G MspI 444 CCGG C^CGG MwoI 388 GCTCTGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 153 GATC ^GATC NspI 377 ACATGC [GA]CATG^[CT] PvuII 68 CAGCTG CAG^CTG RsaI 272 GTAC GT^AC SacI 386 GAGCTC GAGCT^C SapI 525 GAAGAGC GCTCTTC(1/4) Sau3AI 153 GATC ^GATC Sau96I 492 GGCCC G^G[ACGT]CC TaqI 155 TCGA T^CGA TfiI 168 GAATC G^A[AT]TC TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 155 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 313 GAGCAC G[AT]GC[AT]^C 386 GAGCTC BsaJI 494 CCCAGG C^C[ACGT][ACGT]GG 495 CCAGGG BsiHKAI 313 GAGCAC G[AT]GC[AT]^C 386 GAGCTC BssKI 382 CCTGG ^CC[ACGT]GG 495 CCAGG BstF5I 123 CATCC GGATG(2/0) 189 GGATG BstNI 382 CCTGG CC^[AT]GG 495 CCAGG Cac8I 102 GCTTGC GC[ACGT]^[ACGT]GC 489 GCTGGC EarI 292 GAAGAG CTCTTC(1/4) 525 GAAGAG EcoRII 382 CCTGG ^CC[AT]GG 495 CCAGG FokI 123 CATCC GGATG(9/13) 189 GGATG HindIII 100 AAGCTT A^AGCTT 430 AAGCTT Ksp632I 292 GAAGAG CTCTTC(1/4) 525 GAAGAG MvaI 382 CCTGG CC^[AT]GG 495 CCAGG NlaIII 378 CATG CATG^ 534 CATG PleI 64 GACTC GAGTC(4/5) 295 GAGTC ScrFI 382 CCTGG CC^[ACGT]GG 495 CCAGG Tsp45I 346 GTGAC ^GT[GC]AC 440 GTGAC Tsp509I 95 AATT ^AATT 509 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BmyI BsiYI BslI BsmAI Bsp1286I BsrI DdeI HinfI MaeIII MboII MnlI MseI Tru9I