(Linear) (Six-base) MAPSORT of: KIEE4014.seq  Check: 5599  from: 1  to: 576



KIEE4014.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:08  ..

AccI GT'mk_AC

Cuts at:      0      79     576 
   Size:         79     497
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     576 
   Size:        288     288
 
BanII G_rGCy'C

Cuts at:      0     390     576 
   Size:        390     186
 
BbvCI CC'TCA_GC

Cuts at:      0     398     576 
   Size:        398     178
 
Bme1580I G_kGCm'C

Cuts at:      0     474     576 
   Size:        474     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     576 
   Size:        404     172
 
Bpu10I CC'TnA_GC

Cuts at:      0     107     398     576 
   Size:        107     291     178

  Fragments arranged by size: 

                 291     178     107
 
BsaWI w'CCGG_w

Cuts at:      0     443     576 
   Size:        443     133
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     560     576 
   Size:        560      16
 
BseYI C'CCAG_C

Cuts at:      0       9      70     576 
   Size:          9      61     506

  Fragments arranged by size: 

                 506      61       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     317     390     576 
   Size:        317      73     186

  Fragments arranged by size: 

                 317     186      73
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     390     474     576 
   Size:        317      73      84     102

  Fragments arranged by size: 

                 317     102      84      73
 
BtsI GCAGTG_nn'

Cuts at:      0     444     576 
   Size:        444     132
 
EarI CTCTTCn'nnn_

Cuts at:      0     287     520     576 
   Size:        287     233      56

  Fragments arranged by size: 

                 287     233      56
 
EcoICRI GAG'CTC

Cuts at:      0     388     576 
   Size:        388     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     576 
   Size:        404     172
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     131     576 
   Size:        131     445
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      67     576 
   Size:         67     509
 
HindIII A'AGCT_T

Cuts at:      0     100     430     576 
   Size:        100     330     146

  Fragments arranged by size: 

                 330     146     100
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     174     576 
   Size:        140      34     402

  Fragments arranged by size: 

                 402     140      34
 
MslI CAynn'nnrTG

Cuts at:      0     412     576 
   Size:        412     164
 
MspA1I CmG'CkG

Cuts at:      0      70     576 
   Size:         70     506
 
NspI r_CATG'y

Cuts at:      0     381     576 
   Size:        381     195
 
PasI CC'CwG_GG

Cuts at:      0     495     576 
   Size:        495      81
 
PsiI TTA'TAA

Cuts at:      0      92     576 
   Size:         92     484
 
PvuII CAG'CTG

Cuts at:      0      70     576 
   Size:         70     506
 
SacI G_AGCT'C

Cuts at:      0     390     576 
   Size:        390     186
 
SapI GCTCTTCn'nnn_

Cuts at:      0     520     576 
   Size:        520      56
 
TatI w'GTAC_w

Cuts at:      0     271     576 
   Size:        271     305
 

 Enzymes that do cut:

     AccI    AlwNI    BanII    BbvCI Bme1580I     BpmI   Bpu10I    BsaWI
    BseRI    BseYI  BsiHKAI Bsp1286I     BtsI     EarI  EcoICRI  Eco57MI
    EcoNI     FalI  HindIII     MmeI     MslI   MspA1I     NspI     PasI
     PsiI    PvuII     SacI     SapI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI