################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AclI 895 AACGTT AA^CGTT AocI 808 CCTGAGG CC^T[ACGT]AGG AvaI 836 CTCGAG C^[CT]CG[GA]G BbsI 265 GAAGAC GAAGAC(2/6) BmyI 165 GTGCCC G[AGT]GC[ACT]^C BpmI 556 CTCCAG CTGGAG(16/14) BpuAI 265 GAAGAC GAAGAC(2/6) BsaI 141 GAGACC GGTCTC(1/5) BsaJI 534 CCAAGG C^C[ACGT][ACGT]GG BseRI 431 CTCCTC GAGGAG(10/8) BsmFI 29 GTCCC GGGAC(10/14) BsoBI 836 CTCGAG C^[CT]CG[GA]G Bsp1286I 165 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 780 TGTACA T^GTACA BspMI 286 ACCTGC ACCTGC(4/8) BsrDI 355 CATTGC GCAATG(2/0) BsrGI 780 TGTACA T^GTACA BssSI 59 CTCGTG CACGAG(-5/-1) Bsu36I 808 CCTGAGG CC^T[ACGT]AGG Cac8I 773 GCCAGC GC[ACGT]^[ACGT]GC Eco81I 808 CCTGAGG CC^T[ACGT]AGG EcoRI 152 GAATTC G^AATTC EcoT14I 534 CCAAGG C^C[AT][AT]GG HaeIII 947 GGCC GG^CC HpaI 21 GTTAAC GTT^AAC HphI 284 TCACC GGTGA(8/7) MaeII 896 ACGT A^CGT MslI 158 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 33 CATATG CA^TATG NspI 777 GCATGT [GA]CATG^[CT] PaeR7I 836 CTCGAG C^TCGAG PleI 733 GAGTC GAGTC(4/5) Psp1406I 895 AACGTT AA^CGTT SfaNI 118 GCATC GCATC(5/9) SfcI 389 CTATAG C^T[GA][CT]AG SspBI 780 TGTACA T^GTACA SspI 849 AATATT AAT^ATT StyI 534 CCAAGG C^C[AT][AT]GG TfiI 211 GAATC G^A[AT]TC XhoI 836 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 55 GATCC GGATC(4/5) 531 GATCC Asp700I 103 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGCTGTTC BbvI 690 GCAGC GCAGC(8/12) 693 GCAGC BglII 474 AGATCT A^GATCT 785 AGATCT BsmAI 141 GAGAC GTCTC(1/5) 735 GTCTC BssKI 944 CCTGG ^CC[ACGT]GG 949 CCTGG BstNI 944 CCTGG CC^[AT]GG 949 CCTGG DraI 978 TTTAAA TTT^AAA 987 TTTAAA EarI 16 GAAGAG CTCTTC(1/4) 730 GAAGAG EcoRII 944 CCTGG ^CC[AT]GG 949 CCTGG Fnu4HI 690 GCAGC GC^[ACGT]GC 693 GCAGC HinfI 211 GAATC G^A[ACGT]TC 733 GAGTC ItaI 690 GCAGC GC^[ACGT]GC 693 GCAGC Ksp632I 16 GAAGAG CTCTTC(1/4) 730 GAAGAG MvaI 944 CCTGG CC^[AT]GG 949 CCTGG MwoI 167 GCCCTAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 232 GCACTAAGGGC ScrFI 944 CCTGG CC^[ACGT]GG 949 CCTGG SmlI 160 CTCAAG C^T[CT][GA]AG 836 CTCGAG TaqI 837 TCGA T^CGA 1030 TCGA TseI 690 GCAGC G^C[AT]GC 693 GCAGC Tsp45I 283 GTCAC ^GT[GC]AC 1160 GTCAC TspRI 466 CACTG CA[GC]TG[ACGT][ACGT]^ 710 CACTG TthHB8I 837 TCGA T^CGA 1030 TCGA XmnI 103 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BsrI BstF5I BstYI DdeI DpnII FokI HincII HindII MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII PshBI RsaI Sau3AI Tru9I Tsp509I XhoII