(Circular) (Six-base) MAPSORT of: pFN21AE4077.seq Check: 9957 from: 1 to: 5914 pFN21AE4077 5914 bp With 182 enzymes: SgfI * March 28, 2012 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2300 2300 Size: 5914 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3257 1937 Size: 1320 4594 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 2901 4041 4414 1563 Size: 1338 1140 373 3063 Fragments arranged by size: 3063 1338 1140 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 3120 3977 5361 5629 1632 Size: 470 1018 857 1384 268 1917 Fragments arranged by size: 1917 1384 1018 857 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 2167 1166 Size: 1001 4913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5914 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2929 2961 2929 Size: 32 5882 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 2157 2750 2832 2922 2978 2988 3188 3223 Size: 664 593 82 90 56 10 200 35 Cuts at: 3223 3449 3601 5710 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 664 593 226 200 152 90 82 56 35 10 AseI AT'TA_AT Cuts at: 160 1032 2646 2708 3014 4468 160 Size: 872 1614 62 306 1454 1606 Fragments arranged by size: 1614 1606 1454 872 306 62 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2841 3239 1870 Size: 73 898 398 4545 Fragments arranged by size: 4545 898 398 73 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 4690 618 Size: 325 217 627 1448 1455 1842 Fragments arranged by size: 1842 1455 1448 627 325 217 BanII G_rGCy'C Cuts at: 1391 3233 1391 Size: 1842 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2277 928 Size: 971 378 4565 Fragments arranged by size: 4565 971 378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4105 4139 1903 Size: 34 50 34 2084 34 3678 Fragments arranged by size: 3678 2084 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2479 2790 5909 2479 Size: 311 3119 2484 Fragments arranged by size: 3119 2484 311 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 2174 3975 5131 1790 Size: 384 1801 1156 2573 Fragments arranged by size: 2573 1801 1156 384 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3645 4603 442 Size: 416 53 83 399 24 2228 958 1753 Fragments arranged by size: 2228 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 4574 1239 Size: 1307 2028 2579 Fragments arranged by size: 2579 2028 1307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 4039 4904 5202 2150 Size: 1889 865 298 2862 Fragments arranged by size: 2862 1889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 4577 882 Size: 617 641 2437 2219 Fragments arranged by size: 2437 2219 641 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3721 4103 4738 275 Size: 53 83 186 936 2188 382 635 1451 Fragments arranged by size: 2188 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2427 1186 Size: 1241 4673 BseYI C'CCAG_C Cuts at: 5117 5613 5117 Size: 496 5418 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3975 4060 5131 3975 Size: 85 1071 4758 Fragments arranged by size: 4758 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 3233 3975 4060 5131 1391 Size: 399 384 1059 742 85 1071 2174 Fragments arranged by size: 2174 1071 1059 742 399 384 85 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2300 3269 844 Size: 1456 969 3489 Fragments arranged by size: 3489 1456 969 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2359 4409 4583 65 Size: 1183 1111 2050 174 1396 Fragments arranged by size: 2050 1396 1183 1111 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 2785 96 Size: 2689 3225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 2064 3974 4986 2064 Size: 1910 1012 2992 Fragments arranged by size: 2992 1910 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2059 2479 2790 3244 3611 3996 4013 5465 Size: 87 420 311 454 367 385 17 1452 Cuts at: 5465 5551 5563 5909 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 454 420 385 367 346 311 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 2814 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 3430 4224 4244 3430 Size: 794 20 5100 Fragments arranged by size: 5100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 2985 2994 3218 2985 Size: 9 224 5681 Fragments arranged by size: 5681 224 9 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3361 4250 5879 Size: 54 1425 50 275 18 1531 889 1629 Cuts at: 5879 8 Size: 43 Fragments arranged by size: 1629 1531 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2016 2730 3844 1353 Size: 663 714 1114 3423 Fragments arranged by size: 3423 1114 714 663 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2546 3120 3977 4574 5361 5629 Size: 393 470 444 574 857 597 787 268 Cuts at: 5629 1239 Size: 1524 Fragments arranged by size: 1524 857 787 597 574 470 444 393 268 EcoRI G'AATT_C Cuts at: 2157 3223 3601 2157 Size: 1066 378 4470 Fragments arranged by size: 4470 1066 378 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373 Size: 32 135 32 2983 32 42 32 2626 Fragments arranged by size: 2983 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 2378 2460 3258 3504 669 Size: 988 281 90 350 82 798 246 3079 Fragments arranged by size: 3079 988 798 350 281 246 90 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 2028 3504 2028 Size: 1476 4438 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 5028 5212 1305 Size: 923 2800 184 2007 Fragments arranged by size: 2800 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4092 Fragments arranged by size: 4092 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2167 3872 4231 4390 518 Size: 648 1001 1705 359 159 2042 Fragments arranged by size: 2042 1705 1001 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3330 4007 5155 5400 1606 Size: 1724 677 1148 245 2120 Fragments arranged by size: 2120 1724 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5362 NdeI CA'TA_TG Cuts at: 387 2039 387 Size: 1652 4262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2786 3272 4817 1132 Size: 97 1557 486 1545 2229 Fragments arranged by size: 2229 1557 1545 486 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PspXI vC'TCGA_Gb Cuts at: 2841 2841 Size: 5914 PstI C_TGCA'G Cuts at: 830 3266 830 Size: 2436 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2394 3262 4397 5078 5269 826 Size: 219 1349 868 1135 681 191 1471 Fragments arranged by size: 1471 1349 1135 868 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 2841 4018 4919 5181 820 Size: 197 926 222 676 1177 901 262 1553 Fragments arranged by size: 1553 1177 926 901 676 262 222 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 2856 3838 5 Size: 47 2804 982 2081 Fragments arranged by size: 2804 2081 982 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2539 3353 513 Size: 552 1474 814 3074 Fragments arranged by size: 3074 1474 814 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3941 3958 4111 4296 4741 325 Size: 1255 2361 17 153 185 445 1498 Fragments arranged by size: 2361 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2068 2785 4160 Size: 274 80 33 51 1443 91 717 1375 Cuts at: 4160 5872 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1375 717 274 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3179 3211 5874 5906 3179 Size: 32 2663 32 3187 Fragments arranged by size: 3187 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 2841 1943 Size: 898 5016 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 2112 2556 4043 2112 Size: 444 1487 3983 Fragments arranged by size: 3983 1487 444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI