################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TseI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 358 CCGC CCGC(-3/-1) AfaI 615 GTAC GT^AC Asp700I 92 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 683 CCCGAG C^[CT]CG[GA]G AvaII 225 GGACC G^G[AT]CC BalI 663 TGGCCA TGG^CCA BamHI 294 GGATCC G^GATCC BbsI 519 GAAGAC GAAGAC(2/6) BpuAI 519 GAAGAC GAAGAC(2/6) BseRI 589 GAGGAG GAGGAG(10/8) BsiYI 216 CCAGACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 216 CCAGACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 116 GAGAC GTCTC(1/5) BsmFI 758 GGGAC GGGAC(10/14) BsoBI 683 CCCGAG C^[CT]CG[GA]G BsrDI 427 GCAATG GCAATG(2/0) BsrI 523 ACTGG ACTGG(1/-1) BstYI 294 GGATCC [GA]^GATC[CT] Cfr13I 225 GGACC G^G[ACGT]CC EaeI 663 TGGCCA [CT]^GGCC[GA] HphI 178 GGTGA GGTGA(8/7) MflI 294 GGATCC [GA]^GATC[CT] MluNI 663 TGGCCA TGG^CCA MscI 663 TGGCCA TGG^CCA PflMI 216 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 615 GTAC GT^AC Sau96I 225 GGACC G^G[ACGT]CC StuI 374 AGGCCT AGG^CCT TaqI 393 TCGA T^CGA Tsp509I 263 AATT ^AATT TthHB8I 393 TCGA T^CGA Van91I 216 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 666 CCATGAGAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 294 GGATCC [GA]^GATC[CT] XmnI 92 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 295 GATCC GGATC(4/5) 294 GGATC BpmI 398 CTCCAG CTGGAG(16/14) 524 CTGGAG BstF5I 8 GGATG GGATG(2/0) 30 GGATG DpnII 295 GATC ^GATC 743 GATC EarI 113 GAAGAG CTCTTC(1/4) 532 GAAGAG FokI 8 GGATG GGATG(9/13) 30 GGATG HaeIII 375 GGCC GG^CC 664 GGCC HincII 281 GTCAAC GT[CT]^[GA]AC 538 GTCAAC HindII 281 GTCAAC GT[CT]^[GA]AC 538 GTCAAC Ksp632I 113 GAAGAG CTCTTC(1/4) 532 GAAGAG MboI 295 GATC ^GATC 743 GATC MseI 500 TTAA T^TAA 692 TTAA NdeII 295 GATC ^GATC 743 GATC NlaIV 294 GGATCC GG[ACGT]^[ACGT]CC 679 GGCTCC PleI 192 GACTC GAGTC(4/5) 49 GAGTC Sau3AI 295 GATC ^GATC 743 GATC SfaNI 9 GATGC GCATC(5/9) 571 GATGC Tru9I 500 TTAA T^TAA 692 TTAA TspRI 290 CAGTG CA[GC]TG[ACGT][ACGT]^ 444 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AspHI BanII BfaI BmyI BsiHKAI Bsp1286I BssKI BstNI DdeI EcoRII HinfI MaeI MboII MnlI MslI MvaI NlaIII SacI ScrFI TfiI