################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## Acc65I AccIII AciI AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MamI MfeI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 5 GACGTC GACGT^C AccI 62 GTAGAC GT^[AC][GT]AC AccII 386 CGCG CG^CG AcsI 909 AAATTT [GA]^AATT[CT] AcyI 5 GACGTC G[GA]^CG[CT]C AflIII 385 ACGCGT A^C[GA][CT]GT ApoI 909 AAATTT [GA]^AATT[CT] AseI 957 ATTAAT AT^TAAT AsnI 957 ATTAAT AT^TAAT Asp700I 251 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 934 GCTGAGG CCTCAGC(-5/-2) BpmI 231 CTCCAG CTGGAG(16/14) BsaHI 5 GACGTC G[GA]^CG[CT]C BsaI 395 GGTCTC GGTCTC(1/5) BseRI 72 CTCCTC GAGGAG(10/8) BsmBI 337 CGTCTC CGTCTC(1/5) BsmFI 3 GGGAC GGGAC(10/14) BspDI 203 ATCGAT AT^CGAT BstUI 386 CGCG CG^CG Cac8I 197 GCAGGC GC[ACGT]^[ACGT]GC ClaI 203 ATCGAT AT^CGAT DdeI 935 CTGAG C^T[ACGT]AG EarI 219 CTCTTC CTCTTC(1/4) HgaI 194 GACGC GACGC(5/10) Hin1I 5 GACGTC G[GA]^CG[CT]C Ksp632I 219 CTCTTC CTCTTC(1/4) MaeIII 374 GTCAC ^GT[ACGT]AC MluI 385 ACGCGT A^CGCGT MseI 958 TTAA T^TAA MslI 178 CATTCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 386 CGCG CG^CG PaeR7I 75 CTCGAG C^TCGAG PflFI 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 5 GACGTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 957 ATTAAT AT^TAAT SapI 218 GCTCTTC GCTCTTC(1/4) ScaI 832 AGTACT AGT^ACT SmlI 75 CTCGAG C^T[CT][GA]AG Tru9I 958 TTAA T^TAA Tsp45I 374 GTCAC ^GT[GC]AC Tth111I 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 554 CCAGACTACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 75 CTCGAG C^TCGAG XmnI 251 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 833 GTAC GT^AC 879 GTAC AlwI 823 GATCC GGATC(4/5) 850 GATCC AlwNI 17 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 815 CAGTTGCTG AvaI 75 CTCGAG C^[CT]CG[GA]G 398 CTCGGG BbsI 191 GAAGAC GAAGAC(2/6) 649 GAAGAC BbvI 577 GCTGC GCAGC(8/12) 580 GCTGC BfaI 296 CTAG C^TAG 770 CTAG BglII 145 AGATCT A^GATCT 159 AGATCT BpuAI 191 GAAGAC GAAGAC(2/6) 649 GAAGAC BsaJI 662 CCCTGG C^C[ACGT][ACGT]GG 896 CCCTGG BsmAI 338 GTCTC GTCTC(1/5) 396 GTCTC BsoBI 75 CTCGAG C^[CT]CG[GA]G 398 CTCGGG BsrI 371 CCAGT ACTGG(1/-1) 830 CCAGT BstF5I 861 CATCC GGATG(2/0) 904 CATCC Fnu4HI 577 GCTGC GC^[ACGT]GC 580 GCTGC FokI 861 CATCC GGATG(9/13) 904 CATCC HinfI 496 GAATC G^A[ACGT]TC 712 GATTC ItaI 577 GCTGC GC^[ACGT]GC 580 GCTGC MaeI 296 CTAG C^TAG 770 CTAG MspA1I 536 CAGCTG C[AC]G^C[GT]G 562 CAGCTG NlaIV 229 GGCTCC GG[ACGT]^[ACGT]CC 765 GGAACC PvuII 536 CAGCTG CAG^CTG 562 CAGCTG RsaI 833 GTAC GT^AC 879 GTAC SfcI 279 CTATAG C^T[GA][CT]AG 559 CTACAG TfiI 496 GAATC G^A[AT]TC 712 GATTC TseI 577 GCTGC G^C[AT]GC 580 GCTGC TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^ 761 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI AvaII BssKI BstNI BstYI Cfr13I DpnII EcoRII HaeIII MaeII MboI MboII MflI MnlI MvaI MwoI NdeII Sau3AI Sau96I ScrFI SfaNI TaqI Tsp509I TthHB8I XhoII