(Circular) (Six-base) MAPSORT of: pFN21AE6625.seq Check: 8614 from: 1 to: 5707 pFN21AE6625 5707 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2014 3517 278 Size: 53 83 186 936 478 1503 2468 Fragments arranged by size: 2468 1503 936 478 186 83 53 AccI GT'mk_AC Cuts at: 1937 2068 3050 1937 Size: 131 982 4594 Fragments arranged by size: 4594 982 131 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 2076 2153 3770 5154 5422 1632 Size: 417 27 77 1617 1384 268 1917 Fragments arranged by size: 1917 1617 1384 417 268 77 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 2390 4606 5583 1203 Size: 1187 2216 977 1327 Fragments arranged by size: 2216 1327 1187 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2936 2968 2936 Size: 32 5675 AlwNI CAG_nnn'CTG Cuts at: 2027 2825 3450 5022 2027 Size: 798 625 1572 2712 Fragments arranged by size: 2712 1572 798 625 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2914 3016 3242 3394 5503 1493 Size: 1421 102 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 2963 4261 160 Size: 872 1931 1298 1606 Fragments arranged by size: 1931 1606 1298 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2403 3032 1870 Size: 73 137 323 629 4545 Fragments arranged by size: 4545 629 323 137 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2203 2661 928 Size: 971 304 458 3974 Fragments arranged by size: 3974 971 458 304 BbvCI CC'TCA_GC Cuts at: 2940 2940 Size: 5707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2150 2164 5702 2150 Size: 14 3538 2155 Fragments arranged by size: 3538 2155 14 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2829 3438 4396 Size: 416 53 83 399 24 1412 609 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1412 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 4367 1239 Size: 982 2146 2579 Fragments arranged by size: 2579 2146 982 Bpu10I CC'TnA_GC Cuts at: 2940 2940 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2406 4370 882 Size: 617 907 1964 2219 Fragments arranged by size: 2219 1964 907 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2011 3514 3896 4531 Size: 53 83 186 936 478 1503 382 635 Cuts at: 4531 275 Size: 1451 Fragments arranged by size: 1503 1451 936 635 478 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2328 2358 1178 Size: 30 165 30 925 30 4527 Fragments arranged by size: 4527 925 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2068 1186 Size: 882 4825 BseYI C'CCAG_C Cuts at: 4910 5406 4910 Size: 496 5211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2348 2348 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3768 3853 4924 1391 Size: 399 1236 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2150 2164 2854 3037 3404 3789 3806 5258 Size: 178 14 690 183 367 385 17 1452 Cuts at: 5258 5344 5356 5702 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 690 385 367 346 183 178 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2371 3223 4017 4037 2371 Size: 852 794 20 4041 Fragments arranged by size: 4041 852 794 20 ClaI AT'CG_AT Cuts at: 1554 2209 1554 Size: 655 5052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3003 3154 4043 Size: 54 1425 50 275 18 1173 151 889 Cuts at: 4043 5672 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1173 889 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 3637 1353 Size: 877 1407 3423 Fragments arranged by size: 3423 1407 877 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2076 2153 2221 3770 4367 5154 Size: 393 417 27 77 68 1549 597 787 Cuts at: 5154 5422 1239 Size: 268 1524 Fragments arranged by size: 1549 1524 787 597 417 393 268 77 68 27 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2736 2736 Size: 5707 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2328 2360 4348 4380 4422 Size: 32 135 32 756 32 1988 32 42 Cuts at: 4422 4454 1373 Size: 32 2626 Fragments arranged by size: 2626 1988 756 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 2390 5583 2390 Size: 3193 2514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2998 4821 5005 1305 Size: 1693 1823 184 2007 Fragments arranged by size: 2007 1823 1693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 3665 4024 4183 518 Size: 648 1021 1478 359 159 2042 Fragments arranged by size: 2042 1478 1021 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 2569 3123 3800 4948 5193 1606 Size: 937 26 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 554 245 26 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2014 1613 Size: 401 5306 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspXI vC'TCGA_Gb Cuts at: 2080 2080 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2543 2569 2543 Size: 26 5681 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SapI GCTCTTCn'nnn_ Cuts at: 2230 2230 Size: 5707 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 2839 3955 5667 2839 Size: 1116 1712 2879 Fragments arranged by size: 2879 1712 1116 SfcI C'TryA_G Cuts at: 826 1045 2284 2564 3055 4190 4871 5062 826 Size: 219 1239 280 491 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 681 491 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 3811 4712 4974 820 Size: 197 926 137 1731 901 262 1553 Fragments arranged by size: 1731 1553 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 3146 513 Size: 552 939 1142 3074 Fragments arranged by size: 3074 1142 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2004 2388 2848 3734 3751 3904 4089 Size: 1255 424 384 460 886 17 153 185 Cuts at: 4089 4534 325 Size: 445 1498 Fragments arranged by size: 1498 1255 886 460 445 424 384 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2837 3953 5665 Size: 274 80 33 51 1443 860 1116 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1116 860 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 2340 1316 Size: 1024 4683 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2566 2566 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 2080 1943 Size: 137 5570 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2260 3836 2260 Size: 1576 4131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2012 3515 276 Size: 53 83 186 936 478 1503 2468 Fragments arranged by size: 2468 1503 936 478 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI