################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII Hin1I HindIII KasI KpnI KspI MflI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AgeI 729 ACCGGT A^CCGGT AlwNI 535 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 388 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1166 TGCGCA TGC^GCA BcgI 363 CGAAGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1018 CTAG C^TAG BsaBI 318 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 830 GGTCTC GGTCTC(1/5) BseRI 426 GAGGAG GAGGAG(10/8) BsgI 18 CTGCAC GTGCAG(16/14) BsmFI 151 GGGAC GGGAC(10/14) BsrDI 820 GCAATG GCAATG(2/0) BsrFI 729 ACCGGT [GA]^CCGG[CT] BssHII 139 GCGCGC G^CGCGC Cfr10I 729 ACCGGT [GA]^CCGG[CT] DsaI 596 CCATGG C^C[GA][CT]GG EarI 36 GAAGAG CTCTTC(1/4) FspI 1166 TGCGCA TGC^GCA HaeIII 111 GGCC GG^CC HgaI 993 GCGTC GACGC(5/10) HincII 343 GTTAAC GT[CT]^[GA]AC HindII 343 GTTAAC GT[CT]^[GA]AC HpaI 343 GTTAAC GTT^AAC Ksp632I 36 GAAGAG CTCTTC(1/4) MaeI 1018 CTAG C^TAG MaeII 154 ACGT A^CGT MamI 318 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 983 CAATTG C^AATTG MseI 344 TTAA T^TAA MspA1I 784 CAGCTG C[AC]G^C[GT]G MunI 983 CAATTG C^AATTG NcoI 596 CCATGG C^CATGG NheI 1017 GCTAGC G^CTAGC PflFI 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 636 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 729 ACCGGT A^CCGGT PstI 541 CTGCAG CTGCA^G PvuII 784 CAGCTG CAG^CTG SmaI 107 CCCGGG CCC^GGG Tru9I 344 TTAA T^TAA Tsp45I 274 GTGAC ^GT[GC]AC Tth111I 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 636 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 107 CCCGGG C^CCGGG XmaI 107 CCCGGG C^CCGGG XmnI 388 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 138 CGCG CG^CG 140 CGCG AcsI 115 AAATTC [GA]^AATT[CT] 290 AAATTT AfaI 651 GTAC GT^AC 750 GTAC AflIII 624 ACATGT A^C[GA][CT]GT 1137 ACATGT AlwI 723 GATCC GGATC(4/5) 1069 GGATC ApoI 115 AAATTC [GA]^AATT[CT] 290 AAATTT BbvCI 163 CCTCAGC CCTCAGC(-5/-2) 1012 CCTCAGC BcnI 107 CCCGG CC^[GC]GG 108 CCGGG BpmI 532 CTCCAG CTGGAG(16/14) 423 CTGGAG BsaWI 579 ACCGGA [AT]^CCGG[AT] 729 ACCGGT BsiEI 125 CGGTCG CG[GA][CT]^CG 577 CGACCG BsmAI 7 GTCTC GTCTC(1/5) 831 GTCTC BspLU11I 624 ACATGT A^CATGT 1137 ACATGT BstUI 138 CGCG CG^CG 140 CGCG HphI 49 GGTGA GGTGA(8/7) 273 GGTGA MaeIII 274 GTGAC ^GT[ACGT]AC 587 GTAAC MvnI 138 CGCG CG^CG 140 CGCG NciI 107 CCCGG CC^[GC]GG 108 CCGGG NspI 624 ACATGT [GA]CATG^[CT] 1137 ACATGT PaeR7I 547 CTCGAG C^TCGAG 663 CTCGAG RsaI 651 GTAC GT^AC 750 GTAC SacI 75 GAGCTC GAGCT^C 793 GAGCTC SmlI 547 CTCGAG C^T[CT][GA]AG 663 CTCGAG TspRI 899 CACTG CA[GC]TG[ACGT][ACGT]^ 913 CAGTG XhoI 547 CTCGAG C^TCGAG 663 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AluI AspHI AvaI AvaII BanI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp509I TthHB8I