(Circular) (Six-base) MAPSORT of: pFN21AE4381.seq Check: 2844 from: 1 to: 5908 pFN21AE4381 5908 bp With 182 enzymes: SgfI * March 28, 2012 14:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 2927 3971 5355 5623 1632 Size: 780 515 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 780 515 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 2629 3142 4807 5784 1203 Size: 1426 513 1665 977 1327 Fragments arranged by size: 1665 1426 1327 977 513 AgeI A'CCGG_T Cuts at: 2734 4739 2734 Size: 2005 3903 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 2908 1166 Size: 1742 4166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AlwNI CAG_nnn'CTG Cuts at: 2545 3651 5223 2545 Size: 1106 1572 3230 Fragments arranged by size: 3230 1572 1106 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 2120 2295 3217 3443 3595 5704 1493 Size: 627 175 922 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 627 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2112 2552 2668 3233 1870 Size: 73 169 440 116 565 4545 Fragments arranged by size: 4545 565 440 169 116 73 BamHI G'GATC_C Cuts at: 3238 3605 3238 Size: 367 5541 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2663 2881 3036 3110 3229 Size: 325 217 627 876 218 155 74 119 Cuts at: 3229 4684 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 876 627 325 218 217 155 119 74 BanII G_rGCy'C Cuts at: 1391 2084 2534 2802 3227 1391 Size: 693 450 268 425 4072 Fragments arranged by size: 4072 693 450 425 268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BbvCI CC'TCA_GC Cuts at: 2169 3018 2169 Size: 849 5059 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2357 2391 4099 4133 1903 Size: 34 50 34 336 34 1708 34 3678 Fragments arranged by size: 3678 1708 336 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 2666 3969 5125 1790 Size: 876 1303 1156 2573 Fragments arranged by size: 2573 1303 1156 876 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2476 2668 3639 Size: 416 53 83 399 24 1059 192 971 Cuts at: 3639 4597 442 Size: 958 1753 Fragments arranged by size: 1753 1059 971 958 416 399 192 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3026 1056 Size: 1970 3938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2449 2522 4568 1239 Size: 1210 73 2046 2579 Fragments arranged by size: 2579 2046 1210 73 Bpu10I CC'TnA_GC Cuts at: 2169 3018 2169 Size: 849 5059 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4033 4898 5196 4033 Size: 865 298 4745 Fragments arranged by size: 4745 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2841 4571 882 Size: 617 1342 1730 2219 Fragments arranged by size: 2219 1730 1342 617 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 2327 5902 2327 Size: 3575 2333 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3715 4097 4732 275 Size: 53 83 186 936 2182 382 635 1451 Fragments arranged by size: 2182 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2584 2734 4341 4739 5013 5160 5571 2584 Size: 150 1607 398 274 147 411 2921 Fragments arranged by size: 2921 1607 411 398 274 150 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2425 2455 2821 2851 1178 Size: 30 165 30 1022 30 366 30 4235 Fragments arranged by size: 4235 1022 366 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2446 1186 Size: 1260 4648 BseYI C'CCAG_C Cuts at: 5111 5607 5111 Size: 496 5412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2008 1163 Size: 573 272 5063 Fragments arranged by size: 5063 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 2585 3358 4119 5147 1540 Size: 275 186 132 452 773 761 1028 2301 Fragments arranged by size: 2301 1028 773 761 452 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2034 2084 2802 3069 3969 4054 5125 2034 Size: 50 718 267 900 85 1071 2817 Fragments arranged by size: 2817 1071 900 718 267 85 50 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2084 2534 2666 2802 3069 3227 Size: 399 244 50 450 132 136 267 158 Cuts at: 3227 3969 4054 5125 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 267 244 158 136 132 85 50 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2832 4403 4577 65 Size: 1183 1584 1571 174 1396 Fragments arranged by size: 1584 1571 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2734 4552 4739 1700 Size: 110 924 1818 187 2869 Fragments arranged by size: 2869 1818 924 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 2144 1925 Size: 219 5689 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903 Size: 1266 367 385 17 1452 86 12 346 Cuts at: 5903 1972 Size: 1977 Fragments arranged by size: 1977 1452 1266 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2601 513 Size: 552 1536 3820 Fragments arranged by size: 3820 1536 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 2314 2666 487 Size: 683 56 899 189 352 3729 Fragments arranged by size: 3729 899 683 352 189 56 BtsI GCAGTG_nn' Cuts at: 3424 4218 4238 3424 Size: 794 20 5094 Fragments arranged by size: 5094 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873 Size: 54 1425 50 275 18 1525 889 1629 Cuts at: 5873 8 Size: 43 Fragments arranged by size: 1629 1525 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 3838 1353 Size: 683 1802 3423 Fragments arranged by size: 3423 1802 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4495 4869 5015 387 Size: 1064 138 2906 374 146 1280 Fragments arranged by size: 2906 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2082 2800 2082 Size: 718 5190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2449 2522 2927 3971 4568 5355 Size: 393 780 37 73 405 1044 597 787 Cuts at: 5355 5623 1239 Size: 268 1524 Fragments arranged by size: 1524 1044 787 780 597 405 393 268 73 37 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 3173 4414 3173 Size: 1241 4667 HaeII r_GCGC'y Cuts at: 1678 5055 1678 Size: 3377 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 2821 2853 3188 Size: 32 135 32 853 32 364 32 335 Cuts at: 3188 3220 4549 4581 4623 4655 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1329 853 364 335 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 3252 3498 669 Size: 988 281 412 902 246 3079 Fragments arranged by size: 3079 988 902 412 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 2350 3498 2350 Size: 1148 4760 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 2988 3507 2988 Size: 519 5389 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2027 5022 5206 1305 Size: 722 2995 184 2007 Fragments arranged by size: 2995 2007 722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2651 2908 3866 4225 4384 518 Size: 648 1434 51 257 958 359 159 2042 Fragments arranged by size: 2042 1434 958 648 359 257 159 51 MspA1I CmG'CkG Cuts at: 1606 2791 3324 4001 5149 5394 1606 Size: 1185 533 677 1148 245 2120 Fragments arranged by size: 2120 1185 1148 677 533 245 NcoI C'CATG_G Cuts at: 513 1065 2601 513 Size: 552 1536 3820 Fragments arranged by size: 3820 1536 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NheI G'CTAG_C Cuts at: 1052 3022 1052 Size: 1970 3938 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2633 3146 3266 4811 1132 Size: 97 1404 513 120 1545 2229 Fragments arranged by size: 2229 1545 1404 513 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5908 PciI A'CATG_T Cuts at: 2629 3142 4807 2629 Size: 513 1665 3730 Fragments arranged by size: 3730 1665 513 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4369 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspXI vC'TCGA_Gb Cuts at: 2552 2552 Size: 5908 PstI C_TGCA'G Cuts at: 830 2550 3260 830 Size: 1720 710 3478 Fragments arranged by size: 3478 1720 710 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2791 2791 Size: 5908 SacI G_AGCT'C Cuts at: 2084 2802 2084 Size: 718 5190 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 2546 2706 3184 3199 3256 4391 5072 Size: 219 1501 160 478 15 57 1135 681 Cuts at: 5072 5263 826 Size: 191 1471 Fragments arranged by size: 1501 1471 1135 681 478 219 191 160 57 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 2114 3235 1872 Size: 242 1121 4545 Fragments arranged by size: 4545 1121 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 2668 4012 4913 5175 820 Size: 197 926 609 116 1344 901 262 1553 Fragments arranged by size: 1553 1344 926 901 609 262 197 116 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SrfI GCCC'GGGC Cuts at: 2114 2114 Size: 5908 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2601 2641 3106 3347 513 Size: 552 1536 40 465 241 3074 Fragments arranged by size: 3074 1536 552 465 241 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2076 2266 3935 3952 4105 4290 4735 Size: 1255 496 190 1669 17 153 185 445 Cuts at: 4735 325 Size: 1498 Fragments arranged by size: 1669 1498 1255 496 445 190 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 4154 5866 Size: 274 80 33 51 1443 678 1499 1712 Cuts at: 5866 96 Size: 138 Fragments arranged by size: 1712 1499 1443 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 2865 2897 5868 5900 2262 Size: 32 571 32 2971 32 2270 Fragments arranged by size: 2971 2270 571 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2833 1316 Size: 1517 4391 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 2552 2668 1943 Size: 609 116 5183 Fragments arranged by size: 5183 609 116 XmaI C'CCGG_G Cuts at: 1870 2112 3233 1870 Size: 242 1121 4545 Fragments arranged by size: 4545 1121 242 XmnI GAAnn'nnTTC Cuts at: 2397 4037 2397 Size: 1640 4268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI