(Linear) (Six-base) MAPSORT of: KIEE4381.seq Check: 7159 from: 1 to: 1203 KIEE4381.orf With 182 enzymes: SgfI * March 22, 2012 17:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 407 922 1203 Size: 407 515 281 Fragments arranged by size: 515 407 281 AflIII A'CryG_T Cuts at: 0 624 1137 1203 Size: 624 513 66 Fragments arranged by size: 624 513 66 AgeI A'CCGG_T Cuts at: 0 729 1203 Size: 729 474 AleI CACnn'nnGTG Cuts at: 0 903 1203 Size: 903 300 AlwNI CAG_nnn'CTG Cuts at: 0 540 1203 Size: 540 663 ApoI r'AATT_y Cuts at: 0 115 290 1203 Size: 115 175 913 Fragments arranged by size: 913 175 115 AvaI C'yCGr_G Cuts at: 0 107 547 663 1203 Size: 107 440 116 540 Fragments arranged by size: 540 440 116 107 BanI G'GyrC_C Cuts at: 0 658 876 1031 1105 1203 Size: 658 218 155 74 98 Fragments arranged by size: 658 218 155 98 74 BanII G_rGCy'C Cuts at: 0 79 529 797 1203 Size: 79 450 268 406 Fragments arranged by size: 450 406 268 79 BbvCI CC'TCA_GC Cuts at: 0 164 1013 1203 Size: 164 849 190 Fragments arranged by size: 849 190 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 352 386 1203 Size: 352 34 817 Fragments arranged by size: 817 352 34 Bme1580I G_kGCm'C Cuts at: 0 661 1203 Size: 661 542 BmrI ACTGGGnnnn_n' Cuts at: 0 471 663 1203 Size: 471 192 540 Fragments arranged by size: 540 471 192 BmtI G_CTAG'C Cuts at: 0 1021 1203 Size: 1021 182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 444 517 1203 Size: 444 73 686 Fragments arranged by size: 686 444 73 Bpu10I CC'TnA_GC Cuts at: 0 164 1013 1203 Size: 164 849 190 Fragments arranged by size: 849 190 164 BsaI GGTCTCn'nnnn_ Cuts at: 0 836 1203 Size: 836 367 BsaBI GATnn'nnATC Cuts at: 0 322 1203 Size: 322 881 BsaWI w'CCGG_w Cuts at: 0 579 729 1203 Size: 579 150 474 Fragments arranged by size: 579 474 150 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 420 450 816 846 1203 Size: 420 30 366 30 357 Fragments arranged by size: 420 366 357 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 441 1203 Size: 441 762 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 1203 Size: 3 1200 BsiEI CG_ry'CG Cuts at: 0 128 580 1203 Size: 128 452 623 Fragments arranged by size: 623 452 128 BsiHKAI G_wGCw'C Cuts at: 0 29 79 797 1064 1203 Size: 29 50 718 267 139 Fragments arranged by size: 718 267 139 50 29 Bsp1286I G_dGCh'C Cuts at: 0 29 79 529 661 797 1064 1203 Size: 29 50 450 132 136 267 139 Fragments arranged by size: 450 267 139 136 132 50 29 BsrDI GCAATG_nn' Cuts at: 0 827 1203 Size: 827 376 BsrFI r'CCGG_y Cuts at: 0 729 1203 Size: 729 474 BssHII G'CGCG_C Cuts at: 0 139 1203 Size: 139 1064 BtgI C'CryG_G Cuts at: 0 596 1203 Size: 596 607 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 120 309 661 1203 Size: 120 189 352 542 Fragments arranged by size: 542 352 189 120 EarI CTCTTCn'nnn_ Cuts at: 0 31 1203 Size: 31 1172 EcoICRI GAG'CTC Cuts at: 0 77 795 1203 Size: 77 718 408 Fragments arranged by size: 718 408 77 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 407 444 517 922 1203 Size: 407 37 73 405 281 Fragments arranged by size: 407 405 281 73 37 FspI TGC'GCA Cuts at: 0 1168 1203 Size: 1168 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 420 452 816 848 1183 1203 Size: 420 32 364 32 335 20 Fragments arranged by size: 420 364 335 32 32 20 HincII GTy'rAC Cuts at: 0 345 1203 Size: 345 858 HpaI GTT'AAC Cuts at: 0 345 1203 Size: 345 858 MfeI C'AATT_G Cuts at: 0 983 1203 Size: 983 220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 22 1203 Size: 22 1181 MslI CAynn'nnrTG Cuts at: 0 595 646 903 1203 Size: 595 51 257 300 Fragments arranged by size: 595 300 257 51 MspA1I CmG'CkG Cuts at: 0 786 1203 Size: 786 417 NcoI C'CATG_G Cuts at: 0 596 1203 Size: 596 607 NheI G'CTAG_C Cuts at: 0 1017 1203 Size: 1017 186 NspI r_CATG'y Cuts at: 0 628 1141 1203 Size: 628 513 62 Fragments arranged by size: 628 513 62 PciI A'CATG_T Cuts at: 0 624 1137 1203 Size: 624 513 66 Fragments arranged by size: 624 513 66 PflMI CCAn_nnn'nTGG Cuts at: 0 642 1203 Size: 642 561 PspXI vC'TCGA_Gb Cuts at: 0 547 1203 Size: 547 656 PstI C_TGCA'G Cuts at: 0 545 1203 Size: 545 658 PvuII CAG'CTG Cuts at: 0 786 1203 Size: 786 417 SacI G_AGCT'C Cuts at: 0 79 797 1203 Size: 79 718 406 Fragments arranged by size: 718 406 79 SfcI C'TryA_G Cuts at: 0 541 701 1179 1194 1203 Size: 541 160 478 15 9 Fragments arranged by size: 541 478 160 15 9 SmaI CCC'GGG Cuts at: 0 109 1203 Size: 109 1094 SmlI C'TyrA_G Cuts at: 0 547 663 1203 Size: 547 116 540 Fragments arranged by size: 547 540 116 SrfI GCCC'GGGC Cuts at: 0 109 1203 Size: 109 1094 StyI C'CwwG_G Cuts at: 0 596 636 1101 1203 Size: 596 40 465 102 Fragments arranged by size: 596 465 102 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 71 261 1203 Size: 71 190 942 Fragments arranged by size: 942 190 71 TatI w'GTAC_w Cuts at: 0 650 1203 Size: 650 553 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 257 289 860 892 1203 Size: 257 32 571 32 311 Fragments arranged by size: 571 311 257 32 32 Tth111I GACn'n_nGTC Cuts at: 0 828 1203 Size: 828 375 XhoI C'TCGA_G Cuts at: 0 547 663 1203 Size: 547 116 540 Fragments arranged by size: 547 540 116 XmaI C'CCGG_G Cuts at: 0 107 1203 Size: 107 1096 XmnI GAAnn'nnTTC Cuts at: 0 392 1203 Size: 392 811 Enzymes that do cut: AcuI AflIII AgeI AleI AlwNI ApoI AvaI BanI BanII BbvCI BcgI Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BssHII BtgI BtgZI EarI EcoICRI Eco57MI FspI Hin4I HincII HpaI MfeI MmeI MslI MspA1I NcoI NheI NspI PciI PflMI PspXI PstI PvuII SacI SfcI SmaI SmlI SrfI StyI TaqII TatI TstI Tth111I XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI BmgBI BplI BpuEI BsaAI BsaHI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII HindIII KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI XbaI XcmI ZraI