(Circular) (Six-base) MAPSORT of: pFN21AB8949.seq Check: 6295 from: 1 to: 5677 pFN21AB8949 5677 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2652 3020 1937 Size: 715 368 4594 Fragments arranged by size: 4594 715 368 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2582 3740 5124 5392 1632 Size: 950 1158 1384 268 1917 Fragments arranged by size: 1917 1384 1158 950 268 AfeI AGC'GCT Cuts at: 2035 2035 Size: 5677 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2966 4576 5553 1203 Size: 1763 1610 977 1327 Fragments arranged by size: 1763 1610 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2757 1184 Size: 1573 4104 AlwNI CAG_nnn'CTG Cuts at: 2648 2889 3420 4992 2648 Size: 241 531 1572 3333 Fragments arranged by size: 3333 1572 531 241 ApaI G_GGCC'C Cuts at: 2132 2177 2132 Size: 45 5632 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2170 3002 1870 Size: 73 227 832 4545 Fragments arranged by size: 4545 832 227 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2295 2351 2762 2998 Size: 325 217 627 359 149 56 411 236 Cuts at: 2998 4453 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 411 359 325 236 217 149 56 BanII G_rGCy'C Cuts at: 1391 2132 2177 2996 1391 Size: 741 45 819 4072 Fragments arranged by size: 4072 819 741 45 BbeI G_GCGC'C Cuts at: 2355 2355 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 2613 928 Size: 971 585 129 3992 Fragments arranged by size: 3992 971 585 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2768 2802 3868 3902 1903 Size: 34 831 34 1066 34 3678 Fragments arranged by size: 3678 1066 831 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2181 4288 Size: 107 122 71 631 605 487 22 2107 Cuts at: 4288 136 Size: 1525 Fragments arranged by size: 2107 1525 631 605 487 122 107 71 22 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 2132 2151 2177 2298 3738 4894 1790 Size: 342 19 26 121 1440 1156 2573 Fragments arranged by size: 2573 1440 1156 342 121 26 19 BmgBI CAC'GTC Cuts at: 1318 2343 1318 Size: 1025 4652 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2665 3408 4366 Size: 416 53 83 399 24 1248 743 958 Cuts at: 4366 442 Size: 1753 Fragments arranged by size: 1753 1248 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2723 2819 4337 1239 Size: 1484 96 1518 2579 Fragments arranged by size: 2579 1518 1484 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2352 2360 3484 3866 Size: 53 83 186 936 819 8 1124 382 Cuts at: 3866 4501 275 Size: 635 1451 Fragments arranged by size: 1451 1124 936 819 635 382 186 83 53 8 BsaWI w'CCGG_w Cuts at: 2245 4110 4508 4782 4929 5340 2245 Size: 1865 398 274 147 411 2582 Fragments arranged by size: 2582 1865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5677 BseYI C'CCAG_C Cuts at: 2030 2073 2363 4880 5376 2030 Size: 43 290 2517 496 2331 Fragments arranged by size: 2517 2331 496 290 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2365 2435 1163 Size: 573 629 70 4405 Fragments arranged by size: 4405 629 573 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 3127 3888 4916 1540 Size: 275 186 105 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 105 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2151 2177 2298 2996 3738 3823 Size: 399 342 19 26 121 698 742 85 Cuts at: 3823 4894 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 698 399 342 121 85 26 19 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 2854 2877 3566 5296 2854 Size: 23 689 1730 3235 Fragments arranged by size: 3235 1730 689 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2024 2195 2814 4321 4508 1700 Size: 110 214 171 619 1507 187 2869 Fragments arranged by size: 2869 1507 619 214 187 171 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 2219 3737 4749 2219 Size: 1518 1012 3147 Fragments arranged by size: 3147 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2142 2154 2430 513 Size: 552 1077 12 276 3760 Fragments arranged by size: 3760 1077 552 276 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2288 3193 3987 4007 2288 Size: 905 794 20 3958 Fragments arranged by size: 3958 905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DraIII CAC_nnn'GTG Cuts at: 2302 2302 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2680 2871 Size: 54 1425 50 275 18 273 577 191 Cuts at: 2871 3124 4013 5642 8 Size: 253 889 1629 43 Fragments arranged by size: 1629 1425 889 577 275 273 253 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2103 3124 1537 Size: 275 291 1021 4090 Fragments arranged by size: 4090 1021 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 3607 1353 Size: 887 1367 3423 Fragments arranged by size: 3423 1367 887 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2833 4264 4638 4784 387 Size: 1064 138 1244 1431 374 146 1280 Fragments arranged by size: 1431 1280 1244 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2582 2723 2819 3740 4337 5124 5392 Size: 393 950 141 96 921 597 787 268 Cuts at: 5392 1239 Size: 1524 Fragments arranged by size: 1524 950 921 787 597 393 268 141 96 EcoO109I rG'GnC_Cy Cuts at: 2129 2174 2541 2129 Size: 45 367 5265 Fragments arranged by size: 5265 367 45 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FseI GG_CCGG'CC Cuts at: 2199 2199 Size: 5677 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2037 2355 4824 1678 Size: 359 318 2469 2531 Fragments arranged by size: 2531 2469 359 318 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2548 2580 2595 2627 4318 Size: 32 135 32 976 32 15 32 1691 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1691 976 135 42 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2653 3021 3267 669 Size: 988 281 715 368 246 3079 Fragments arranged by size: 3079 988 715 368 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2351 2351 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2682 10 Size: 54 1425 343 850 3005 Fragments arranged by size: 3005 1425 850 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 2859 3635 3994 4153 518 Size: 648 1530 163 776 359 159 2042 Fragments arranged by size: 2042 1530 776 648 359 163 159 MspA1I CmG'CkG Cuts at: 1606 2144 2156 3093 3770 4918 5163 1606 Size: 538 12 937 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 538 245 12 NaeI GCC'GGC Cuts at: 2197 2197 Size: 5677 NarI GG'CG_CC Cuts at: 2352 2352 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 2483 387 Size: 2096 3581 NgoMIV G'CCGG_C Cuts at: 2195 2195 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2970 3035 4580 1132 Size: 97 1741 65 1545 2229 Fragments arranged by size: 2229 1741 1545 97 65 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 2966 4576 2966 Size: 1610 4067 PflMI CCAn_nnn'nTGG Cuts at: 1108 2516 1108 Size: 1408 4269 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2541 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2128 2173 2128 Size: 45 5632 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2774 2806 2774 Size: 32 5645 PstI C_TGCA'G Cuts at: 830 2051 2283 3029 830 Size: 1221 232 746 3478 Fragments arranged by size: 3478 1221 746 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SacII CC_GC'GG Cuts at: 2145 2157 2145 Size: 12 5665 SalI G'TCGA_C Cuts at: 1936 2651 3019 1936 Size: 715 368 4594 Fragments arranged by size: 4594 715 368 SanDI GG'GwC_CC Cuts at: 2541 2541 Size: 5677 SapI GCTCTTCn'nnn_ Cuts at: 2240 2240 Size: 5677 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SexAI A'CCwGG_T Cuts at: 2298 2298 Size: 5677 SfcI C'TryA_G Cuts at: 826 1045 2047 2279 2625 2791 3025 4160 4841 Size: 219 1002 232 346 166 234 1135 681 Cuts at: 4841 5032 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1002 681 346 234 232 219 191 166 SfoI GGC'GCC Cuts at: 2353 2353 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2941 3116 513 Size: 552 1876 175 3074 Fragments arranged by size: 3074 1876 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3704 3721 3874 4059 4504 325 Size: 1255 1067 1057 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1067 1057 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3923 5635 96 Size: 274 80 33 51 1443 1946 1712 138 Fragments arranged by size: 1946 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 5637 5669 2069 Size: 32 3536 32 2077 Fragments arranged by size: 3536 2077 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2261 2612 1316 Size: 945 351 4381 Fragments arranged by size: 4381 945 351 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SfiI SrfI SwaI XcmI