(Linear) (Six-base) MAPSORT of: KIBB8949.seq Check: 1672 from: 1 to: 972 KIBB8949.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AccI GT'mk_AC Cuts at: 0 647 972 Size: 647 325 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 972 Size: 577 395 AfeI AGC'GCT Cuts at: 0 30 972 Size: 30 942 AflIII A'CryG_T Cuts at: 0 961 972 Size: 961 11 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 752 972 Size: 752 220 AlwNI CAG_nnn'CTG Cuts at: 0 643 884 972 Size: 643 241 88 Fragments arranged by size: 643 241 88 ApaI G_GGCC'C Cuts at: 0 127 172 972 Size: 127 45 800 Fragments arranged by size: 800 127 45 AvaI C'yCGr_G Cuts at: 0 165 972 Size: 165 807 BanI G'GyrC_C Cuts at: 0 141 290 346 757 972 Size: 141 149 56 411 215 Fragments arranged by size: 411 215 149 141 56 BanII G_rGCy'C Cuts at: 0 127 172 972 Size: 127 45 800 Fragments arranged by size: 800 127 45 BbeI G_GCGC'C Cuts at: 0 350 972 Size: 350 622 BbsI GAAGACnn'nnnn_ Cuts at: 0 479 608 972 Size: 479 129 364 Fragments arranged by size: 479 364 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 763 797 972 Size: 763 34 175 Fragments arranged by size: 763 175 34 BglI GCCn_nnn'nGGC Cuts at: 0 154 176 972 Size: 154 22 796 Fragments arranged by size: 796 154 22 Bme1580I G_kGCm'C Cuts at: 0 127 146 172 293 972 Size: 127 19 26 121 679 Fragments arranged by size: 679 127 121 26 19 BmgBI CAC'GTC Cuts at: 0 338 972 Size: 338 634 BmrI ACTGGGnnnn_n' Cuts at: 0 660 972 Size: 660 312 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 240 972 Size: 240 732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 814 972 Size: 718 96 158 Fragments arranged by size: 718 158 96 BsaHI Gr'CG_yC Cuts at: 0 347 355 972 Size: 347 8 617 Fragments arranged by size: 617 347 8 BsaWI w'CCGG_w Cuts at: 0 240 972 Size: 240 732 BseYI C'CCAG_C Cuts at: 0 25 68 358 972 Size: 25 43 290 614 Fragments arranged by size: 614 290 43 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 430 972 Size: 360 70 542 Fragments arranged by size: 542 360 70 BsiEI CG_ry'CG Cuts at: 0 101 972 Size: 101 871 Bsp1286I G_dGCh'C Cuts at: 0 127 146 172 293 972 Size: 127 19 26 121 679 Fragments arranged by size: 679 127 121 26 19 BspHI T'CATG_A Cuts at: 0 849 872 972 Size: 849 23 100 Fragments arranged by size: 849 100 23 BsrFI r'CCGG_y Cuts at: 0 19 190 809 972 Size: 19 171 619 163 Fragments arranged by size: 619 171 163 19 BssSI C'ACGA_G Cuts at: 0 214 972 Size: 214 758 BtgI C'CryG_G Cuts at: 0 137 149 425 972 Size: 137 12 276 547 Fragments arranged by size: 547 276 137 12 BtsI GCAGTG_nn' Cuts at: 0 283 972 Size: 283 689 DraIII CAC_nnn'GTG Cuts at: 0 297 972 Size: 297 675 EaeI y'GGCC_r Cuts at: 0 98 675 866 972 Size: 98 577 191 106 Fragments arranged by size: 577 191 106 98 EagI C'GGCC_G Cuts at: 0 98 972 Size: 98 874 EarI CTCTTCn'nnn_ Cuts at: 0 235 972 Size: 235 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 828 972 Size: 828 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 718 814 972 Size: 577 141 96 158 Fragments arranged by size: 577 158 141 96 EcoO109I rG'GnC_Cy Cuts at: 0 124 169 536 972 Size: 124 45 367 436 Fragments arranged by size: 436 367 124 45 FseI GG_CCGG'CC Cuts at: 0 194 972 Size: 194 778 HaeII r_GCGC'y Cuts at: 0 32 350 972 Size: 32 318 622 Fragments arranged by size: 622 318 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 543 575 590 622 972 Size: 543 32 15 32 350 Fragments arranged by size: 543 350 32 32 15 HincII GTy'rAC Cuts at: 0 648 972 Size: 648 324 KasI G'GCGC_C Cuts at: 0 346 972 Size: 346 626 MscI TGG'CCA Cuts at: 0 677 972 Size: 677 295 MslI CAynn'nnrTG Cuts at: 0 691 854 972 Size: 691 163 118 Fragments arranged by size: 691 163 118 MspA1I CmG'CkG Cuts at: 0 139 151 972 Size: 139 12 821 Fragments arranged by size: 821 139 12 NaeI GCC'GGC Cuts at: 0 192 972 Size: 192 780 NarI GG'CG_CC Cuts at: 0 347 972 Size: 347 625 NdeI CA'TA_TG Cuts at: 0 478 972 Size: 478 494 NgoMIV G'CCGG_C Cuts at: 0 190 972 Size: 190 782 NspI r_CATG'y Cuts at: 0 965 972 Size: 965 7 PciI A'CATG_T Cuts at: 0 961 972 Size: 961 11 PflMI CCAn_nnn'nTGG Cuts at: 0 511 972 Size: 511 461 PpuMI rG'GwC_Cy Cuts at: 0 536 972 Size: 536 436 PspOMI G'GGCC_C Cuts at: 0 123 168 972 Size: 123 45 804 Fragments arranged by size: 804 123 45 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 769 801 972 Size: 769 32 171 Fragments arranged by size: 769 171 32 PstI C_TGCA'G Cuts at: 0 46 278 972 Size: 46 232 694 Fragments arranged by size: 694 232 46 SacII CC_GC'GG Cuts at: 0 140 152 972 Size: 140 12 820 Fragments arranged by size: 820 140 12 SalI G'TCGA_C Cuts at: 0 646 972 Size: 646 326 SanDI GG'GwC_CC Cuts at: 0 536 972 Size: 536 436 SapI GCTCTTCn'nnn_ Cuts at: 0 235 972 Size: 235 737 SexAI A'CCwGG_T Cuts at: 0 293 972 Size: 293 679 SfcI C'TryA_G Cuts at: 0 42 274 620 786 972 Size: 42 232 346 166 186 Fragments arranged by size: 346 232 186 166 42 SfoI GGC'GCC Cuts at: 0 348 972 Size: 348 624 StyI C'CwwG_G Cuts at: 0 936 972 Size: 936 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 642 972 Size: 642 330 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 64 96 972 Size: 64 32 876 Fragments arranged by size: 876 64 32 Tth111I GACn'n_nGTC Cuts at: 0 256 607 972 Size: 256 351 365 Fragments arranged by size: 365 351 256 Enzymes that do cut: AccI AcuI AfeI AflIII AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI BsaHI BsaWI BseYI BsgI BsiEI Bsp1286I BspHI BsrFI BssSI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FseI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI PpuMI PspOMI PsrI PstI SacII SalI SanDI SapI SexAI SfcI SfoI StyI TaqII TstI Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI PvuII RsrII SacI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmaI XmnI ZraI