Restriction map for
pFN21AE1967
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BclI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
DraI Eam1105I EarI EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SspBI SspI StuI
SwaI Tru9I Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AflIII 503 ACGTGT A^C[GA][CT]GT
AlwNI 856 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1540 CCTGAGG CC^T[ACGT]AGG
Aor51HI 661 AGCGCT AGC^GCT
Asp700I 144 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1291 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1067 TGCGCA TGC^GCA
BamHI 1022 GGATCC G^GATCC
BbrPI 513 CACGTG CAC^GTG
BbvCI 1557 CCTCAGC CCTCAGC(-5/-2)
BcgI 803 GCAAGGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 612 GTATCC GTATCC(6/5)
BfaI 901 CTAG C^TAG
BlpI 657 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 657 GCTGAGC GC^T[ACGT]AGC
BsgI 1415 CTGCAC GTGCAG(16/14)
BsmBI 1585 CGTCTC CGTCTC(1/5)
BssHII 35 GCGCGC G^CGCGC
BstYI 1022 GGATCC [GA]^GATC[CT]
Bsu36I 1540 CCTGAGG CC^T[ACGT]AGG
CelII 657 GCTGAGC GC^T[ACGT]AGC
CpoI 1450 CGGACCG CG^G[AT]CCG
DraII 1277 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 329 CGGCCG C^GGCCG
EclXI 329 CGGCCG C^GGCCG
Eco47III 661 AGCGCT AGC^GCT
Eco52I 329 CGGCCG C^GGCCG
Eco81I 1540 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1277 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1505 CCATGG C^C[AT][AT]GG
FspI 1067 TGCGCA TGC^GCA
HincII 1531 GTCAAC GT[CT]^[GA]AC
HindII 1531 GTCAAC GT[CT]^[GA]AC
HindIII 1123 AAGCTT A^AGCTT
HinfI 558 GAATC G^A[ACGT]TC
MaeI 901 CTAG C^TAG
MaeIII 780 GTCAC ^GT[ACGT]AC
MflI 1022 GGATCC [GA]^GATC[CT]
NcoI 1505 CCATGG C^CATGG
PflFI 1291 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 513 CACGTG CAC^GTG
PmlI 513 CACGTG CAC^GTG
PpuMI 1277 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 1450 CGGACCG CG^G[AT]CCG
SbfI 633 CCTGCAGG CCTGCA^GG
SfaNI 743 GCATC GCATC(5/9)
SmaI 30 CCCGGG CCC^GGG
SmlI 973 CTCAAG C^T[CT][GA]AG
SpeI 900 ACTAGT A^CTAGT
SphI 1094 GCATGC GCATG^C
Sse8387I 633 CCTGCAGG CCTGCA^GG
StyI 1505 CCATGG C^C[AT][AT]GG
TfiI 558 GAATC G^A[AT]TC
Tsp45I 780 GTCAC ^GT[GC]AC
Tth111I 1291 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1022 GGATCC [GA]^GATC[CT]
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
XmnI 144 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 688 GTCTTC GAAGAC(2/6)
922 GTCTTC
BpmI 52 CTCCAG CTGGAG(16/14)
446 CTCCAG
BpuAI 688 GTCTTC GAAGAC(2/6)
922 GTCTTC
BsaAI 502 TACGTG [CT]AC^GT[GA]
513 CACGTG
BsiEI 329 CGGCCG CG[GA][CT]^CG
828 CGACCG
BsrBI 1026 CCGCTC CCGCTC(-3/-3)
1525 GAGCGG
BsrDI 452 GCAATG GCAATG(2/0)
1421 GCAATG
BstAPI 839 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1417 GCACGCAATGC
BstXI 761 CCAACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1119 CCACAAGCTTGG
DraIII 510 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
985 CACTTGGTG
EaeI 329 CGGCCG [CT]^GGCC[GA]
1508 TGGCCG
PvuII 303 CAGCTG CAG^CTG
493 CAGCTG
SacI 20 GAGCTC GAGCT^C
1085 GAGCTC
SexAI 1259 ACCTGGT A^CC[AT]GGT
1280 ACCTGGT
TaqI 872 TCGA T^CGA
926 TCGA
TthHB8I 872 TCGA T^CGA
926 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DrdI DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI KspI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PstI RsaI SacII Sau3AI
Sau96I ScrFI SfcI SfoI TseI
TspRI Van91I XcmI