(Circular) (Six-base) MAPSORT of: pFN21AE1967.seq Check: 7520 from: 1 to: 6295 pFN21AE1967 6295 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4358 5742 6010 1632 Size: 2726 1384 268 1917 Fragments arranged by size: 2726 1917 1384 268 AfeI AGC'GCT Cuts at: 2668 2668 Size: 6295 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 2508 5194 6171 1203 Size: 1305 2686 977 1327 Fragments arranged by size: 2686 1327 1305 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3590 1184 Size: 2406 3889 AlwNI CAG_nnn'CTG Cuts at: 2866 4038 5610 2866 Size: 1172 1572 3551 Fragments arranged by size: 3551 1572 1172 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 3604 3830 3982 6091 1493 Size: 2111 226 152 2109 1697 Fragments arranged by size: 2111 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2466 2567 3388 3620 1870 Size: 73 92 431 101 821 232 4545 Fragments arranged by size: 4545 821 431 232 101 92 73 BamHI G'GATC_C Cuts at: 3027 3625 3992 3027 Size: 598 367 5330 Fragments arranged by size: 5330 598 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2282 2316 2471 2947 Size: 325 217 627 322 173 34 155 476 Cuts at: 2947 3134 3616 5071 618 Size: 187 482 1455 1842 Fragments arranged by size: 1842 1455 627 482 476 325 322 217 187 173 155 34 BanII G_rGCy'C Cuts at: 1391 2029 3094 3350 3395 3614 1391 Size: 638 1065 256 45 219 4072 Fragments arranged by size: 4072 1065 638 256 219 45 BbeI G_GCGC'C Cuts at: 2113 2320 2951 3138 2113 Size: 207 631 187 5270 Fragments arranged by size: 5270 631 207 187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2686 2920 928 Size: 971 787 234 4303 Fragments arranged by size: 4303 971 787 234 BbvCI CC'TCA_GC Cuts at: 3563 3563 Size: 6295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2797 2831 4486 4520 1903 Size: 34 860 34 1655 34 3678 Fragments arranged by size: 3678 1655 860 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2628 4187 5397 2628 Size: 1559 1210 3526 Fragments arranged by size: 3526 1559 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2405 2596 2715 Size: 107 122 71 631 605 733 191 119 Cuts at: 2715 3437 4906 136 Size: 722 1469 1525 Fragments arranged by size: 1525 1469 733 722 631 605 191 122 119 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 2663 3712 2663 Size: 1049 5246 Bme1580I G_kGCm'C Cuts at: 1790 2089 2474 2855 4356 5512 1790 Size: 299 385 381 1501 1156 2573 Fragments arranged by size: 2573 1501 1156 385 381 299 BmgBI CAC'GTC Cuts at: 1318 2935 1318 Size: 1617 4678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2436 4955 1239 Size: 803 394 2519 2579 Fragments arranged by size: 2579 2519 803 394 Bpu10I CC'TnA_GC Cuts at: 3563 3563 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2963 4420 5285 5583 2963 Size: 1457 865 298 3675 Fragments arranged by size: 3675 1457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2955 3075 3288 4958 882 Size: 617 1456 120 213 1670 2219 Fragments arranged by size: 2219 1670 1456 617 213 120 BsaAI yAC'GTr Cuts at: 493 1204 2509 2520 4086 493 Size: 711 1305 11 1566 2702 Fragments arranged by size: 2702 1566 1305 711 11 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2110 2119 2317 2481 Size: 53 83 186 936 577 9 198 164 Cuts at: 2481 2628 2948 3135 4102 4484 5119 275 Size: 147 320 187 967 382 635 1451 Fragments arranged by size: 1451 967 936 635 577 382 320 198 187 186 164 147 83 53 9 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3285 3315 1178 Size: 30 165 30 1882 30 4158 Fragments arranged by size: 4158 1882 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2894 2897 3385 3388 3506 3563 1186 Size: 1708 3 488 3 118 57 3918 Fragments arranged by size: 3918 1708 488 118 57 3 3 BseYI C'CCAG_C Cuts at: 2278 2310 2743 5498 5994 2278 Size: 32 433 2755 496 2579 Fragments arranged by size: 2755 2579 496 433 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3405 1163 Size: 573 1669 4053 Fragments arranged by size: 4053 1669 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 2836 3745 4506 5534 1540 Size: 275 186 336 499 909 761 1028 2301 Fragments arranged by size: 2301 1028 909 761 499 336 275 186 BsiHKAI G_wGCw'C Cuts at: 2029 2208 2353 2783 3094 4356 4441 5512 2029 Size: 179 145 430 311 1262 85 1071 2812 Fragments arranged by size: 2812 1262 1071 430 311 179 145 85 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 BsmBI CGTCTCn'nnnn_ Cuts at: 3596 3596 Size: 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2089 2208 2353 2474 2783 2855 Size: 399 239 60 119 145 121 309 72 Cuts at: 2855 3094 3350 3395 3614 4356 4441 5512 1391 Size: 239 256 45 219 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 256 239 239 219 145 121 119 85 72 60 45 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3650 844 Size: 2806 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3033 3532 4182 1394 Size: 429 1210 499 650 3507 Fragments arranged by size: 3507 1210 650 499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2464 3433 4790 4964 65 Size: 1183 1216 969 1357 174 1396 Fragments arranged by size: 1396 1357 1216 1183 969 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 2040 1925 Size: 115 6180 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2850 3428 794 Size: 2056 578 3661 Fragments arranged by size: 3661 2056 578 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstXI CCAn_nnnn'nTGG Cuts at: 2773 3131 2773 Size: 358 5937 BstYI r'GATC_y Cuts at: 1972 3027 3625 3992 4377 4394 5846 5932 5944 Size: 1055 598 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1055 598 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3546 3546 Size: 6295 BtgI C'CryG_G Cuts at: 513 1065 2421 2712 3040 3117 3510 513 Size: 552 1356 291 328 77 393 3298 Fragments arranged by size: 3298 1356 552 393 328 291 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 2105 2192 2264 2951 3811 4605 4625 2105 Size: 87 72 687 860 794 20 3775 Fragments arranged by size: 3775 860 794 687 87 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DraIII CAC_nnn'GTG Cuts at: 2520 2995 2520 Size: 475 5820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2690 2939 2981 5302 809 Size: 507 1374 249 42 2321 1802 Fragments arranged by size: 2321 1802 1374 507 249 42 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 3513 3742 Size: 54 1425 50 275 18 504 1179 229 Cuts at: 3742 4631 6260 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1179 889 504 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2334 3742 1537 Size: 275 522 1408 4090 Fragments arranged by size: 4090 1408 522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4225 1353 Size: 2872 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3469 4882 5256 5402 387 Size: 1064 138 1880 1413 374 146 1280 Fragments arranged by size: 1880 1413 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2027 3092 2027 Size: 1065 5230 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2436 4358 4955 5742 6010 1239 Size: 393 410 394 1922 597 787 268 1524 Fragments arranged by size: 1922 1524 787 597 410 394 393 268 EcoO109I rG'GnC_Cy Cuts at: 3283 3283 Size: 6295 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 FspI TGC'GCA Cuts at: 3074 4801 3074 Size: 1727 4568 HaeII r_GCGC'y Cuts at: 1678 2113 2171 2320 2527 2670 2866 2951 3138 Size: 435 58 149 207 143 196 85 187 Cuts at: 3138 3186 5442 1678 Size: 48 2256 2531 Fragments arranged by size: 2531 2256 435 207 196 187 149 143 85 58 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3285 3317 4936 4968 5010 Size: 32 135 32 1713 32 1619 32 42 Cuts at: 5010 5042 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 1619 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3538 3639 3885 669 Size: 988 281 1600 101 246 3079 Fragments arranged by size: 3079 1600 988 281 246 101 HindIII A'AGCT_T Cuts at: 748 3128 748 Size: 2380 3915 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KasI G'GCGC_C Cuts at: 2109 2316 2947 3134 2109 Size: 207 631 187 5270 Fragments arranged by size: 5270 631 207 187 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4473 Fragments arranged by size: 4473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4253 4612 4771 518 Size: 648 3087 359 159 2042 Fragments arranged by size: 3087 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 2251 2278 2291 2310 2393 2423 2500 Size: 518 127 27 13 19 83 30 77 Cuts at: 2500 2679 2714 2721 3119 3711 4388 5536 5781 Size: 179 35 7 398 592 677 1148 245 Cuts at: 5781 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 592 518 398 245 179 127 83 77 35 30 27 19 13 7 NarI GG'CG_CC Cuts at: 2110 2317 2948 3135 2110 Size: 207 631 187 5270 Fragments arranged by size: 5270 631 207 187 NcoI C'CATG_G Cuts at: 513 1065 3510 513 Size: 552 2445 3298 Fragments arranged by size: 3298 2445 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2603 2662 3103 3653 5198 1132 Size: 97 1374 59 441 550 1545 2229 Fragments arranged by size: 2229 1545 1374 550 441 97 59 PasI CC'CwG_GG Cuts at: 1348 2321 1348 Size: 973 5322 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2459 3178 3265 3450 3511 1108 Size: 1351 719 87 185 61 3892 Fragments arranged by size: 3892 1351 719 185 87 61 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PmlI CAC'GTG Cuts at: 2520 2520 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3283 3283 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PstI C_TGCA'G Cuts at: 830 2074 2256 2419 2643 3225 3237 3255 3647 Size: 1244 182 163 224 582 12 18 392 Cuts at: 3647 830 Size: 3478 Fragments arranged by size: 3478 1244 582 392 224 182 163 18 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2310 2500 2310 Size: 190 6105 RsrII CG'GwC_CG Cuts at: 3456 3456 Size: 6295 SacI G_AGCT'C Cuts at: 2029 3094 2029 Size: 1065 5230 SacII CC_GC'GG Cuts at: 2424 2715 3120 2424 Size: 291 405 5599 Fragments arranged by size: 5599 405 291 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 2643 3647 2643 Size: 1004 5291 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SexAI A'CCwGG_T Cuts at: 3264 3285 3264 Size: 21 6274 SfcI C'TryA_G Cuts at: 826 1045 2070 2252 2415 2639 3062 3221 3233 Size: 219 1025 182 163 224 423 159 12 Cuts at: 3233 3251 3643 4778 5459 5650 826 Size: 18 392 1135 681 191 1471 Fragments arranged by size: 1471 1135 1025 681 423 392 224 219 191 182 163 159 18 12 SfoI GGC'GCC Cuts at: 2111 2318 2949 3136 2111 Size: 207 631 187 5270 Fragments arranged by size: 5270 631 207 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 2037 3622 1872 Size: 165 1585 4545 Fragments arranged by size: 4545 1585 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 2978 4399 5300 5562 820 Size: 197 926 1035 1421 901 262 1553 Fragments arranged by size: 1553 1421 1035 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 2905 152 Size: 2753 3542 SphI G_CATG'C Cuts at: 1132 3103 3653 1132 Size: 1971 550 3774 Fragments arranged by size: 3774 1971 550 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 3510 3734 513 Size: 552 2445 224 3074 Fragments arranged by size: 3074 2445 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 4322 4339 4492 4677 5122 325 Size: 1255 690 2052 17 153 185 445 1498 Fragments arranged by size: 2052 1498 1255 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4541 6253 96 Size: 274 80 33 51 1443 2564 1712 138 Fragments arranged by size: 2564 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2136 2168 2219 2251 3327 3359 6255 6287 2136 Size: 32 51 32 1076 32 2896 32 2144 Fragments arranged by size: 2896 2144 1076 51 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3299 1316 Size: 1983 4312 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2121 2770 3409 3517 2121 Size: 649 639 108 4899 Fragments arranged by size: 4899 649 639 108 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 2035 3620 1870 Size: 165 1585 4545 Fragments arranged by size: 4545 1585 165 XmnI GAAnn'nnTTC Cuts at: 2153 4424 2153 Size: 2271 4024 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI SanDI SapI SfiI SrfI SwaI