(Linear) (Six-base) MAPSORT of: KIEE1967.seq Check: 3208 from: 1 to: 1590 KIEE1967.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AfeI AGC'GCT Cuts at: 0 663 1590 Size: 663 927 AflIII A'CryG_T Cuts at: 0 503 1590 Size: 503 1087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1585 1590 Size: 1585 5 AlwNI CAG_nnn'CTG Cuts at: 0 861 1590 Size: 861 729 AvaI C'yCGr_G Cuts at: 0 30 461 562 1383 1590 Size: 30 431 101 821 207 Fragments arranged by size: 821 431 207 101 30 BamHI G'GATC_C Cuts at: 0 1022 1590 Size: 1022 568 BanI G'GyrC_C Cuts at: 0 104 277 311 466 942 1129 1590 Size: 104 173 34 155 476 187 461 Fragments arranged by size: 476 461 187 173 155 104 34 BanII G_rGCy'C Cuts at: 0 24 1089 1345 1390 1590 Size: 24 1065 256 45 200 Fragments arranged by size: 1065 256 200 45 24 BbeI G_GCGC'C Cuts at: 0 108 315 946 1133 1590 Size: 108 207 631 187 457 Fragments arranged by size: 631 457 207 187 108 BbsI GAAGACnn'nnnn_ Cuts at: 0 681 915 1590 Size: 681 234 675 Fragments arranged by size: 681 675 234 BbvCI CC'TCA_GC Cuts at: 0 1558 1590 Size: 1558 32 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 792 826 1590 Size: 792 34 764 Fragments arranged by size: 792 764 34 BciVI GTATCCnnnnn_n' Cuts at: 0 623 1590 Size: 623 967 BglI GCCn_nnn'nGGC Cuts at: 0 400 591 710 1432 1590 Size: 400 191 119 722 158 Fragments arranged by size: 722 400 191 158 119 BlpI GC'TnA_GC Cuts at: 0 658 1590 Size: 658 932 Bme1580I G_kGCm'C Cuts at: 0 84 469 850 1590 Size: 84 385 381 740 Fragments arranged by size: 740 385 381 84 BmgBI CAC'GTC Cuts at: 0 930 1590 Size: 930 660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 431 1590 Size: 37 394 1159 Fragments arranged by size: 1159 394 37 Bpu10I CC'TnA_GC Cuts at: 0 1558 1590 Size: 1558 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 958 1590 Size: 958 632 BsaI GGTCTCn'nnnn_ Cuts at: 0 950 1070 1283 1590 Size: 950 120 213 307 Fragments arranged by size: 950 307 213 120 BsaAI yAC'GTr Cuts at: 0 504 515 1590 Size: 504 11 1075 Fragments arranged by size: 1075 504 11 BsaHI Gr'CG_yC Cuts at: 0 105 114 312 476 623 943 1130 1590 Size: 105 9 198 164 147 320 187 460 Fragments arranged by size: 460 320 198 187 164 147 105 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1280 1310 1590 Size: 1280 30 280 Fragments arranged by size: 1280 280 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 892 1380 1383 1501 1558 1590 Size: 889 3 488 3 118 57 32 Fragments arranged by size: 889 488 118 57 32 3 3 BseYI C'CCAG_C Cuts at: 0 273 305 738 1590 Size: 273 32 433 852 Fragments arranged by size: 852 433 273 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1400 1590 Size: 1400 190 BsiEI CG_ry'CG Cuts at: 0 332 831 1590 Size: 332 499 759 Fragments arranged by size: 759 499 332 BsiHKAI G_wGCw'C Cuts at: 0 24 203 348 778 1089 1590 Size: 24 179 145 430 311 501 Fragments arranged by size: 501 430 311 179 145 24 Bsp1286I G_dGCh'C Cuts at: 0 24 84 203 348 469 778 850 1089 Size: 24 60 119 145 121 309 72 239 Cuts at: 1089 1345 1390 1590 Size: 256 45 200 Fragments arranged by size: 309 256 239 200 145 121 119 72 60 45 24 BsrBI CCG'CTC Cuts at: 0 1028 1527 1590 Size: 1028 499 63 Fragments arranged by size: 1028 499 63 BsrDI GCAATG_nn' Cuts at: 0 459 1428 1590 Size: 459 969 162 Fragments arranged by size: 969 459 162 BssHII G'CGCG_C Cuts at: 0 35 1590 Size: 35 1555 BstAPI GCAn_nnn'nTGC Cuts at: 0 845 1423 1590 Size: 845 578 167 Fragments arranged by size: 845 578 167 BstXI CCAn_nnnn'nTGG Cuts at: 0 768 1126 1590 Size: 768 358 464 Fragments arranged by size: 768 464 358 BstYI r'GATC_y Cuts at: 0 1022 1590 Size: 1022 568 Bsu36I CC'TnA_GG Cuts at: 0 1541 1590 Size: 1541 49 BtgI C'CryG_G Cuts at: 0 416 707 1035 1112 1505 1590 Size: 416 291 328 77 393 85 Fragments arranged by size: 416 393 328 291 85 77 BtsI GCAGTG_nn' Cuts at: 0 100 187 259 946 1590 Size: 100 87 72 687 644 Fragments arranged by size: 687 644 100 87 72 DraIII CAC_nnn'GTG Cuts at: 0 515 990 1590 Size: 515 475 600 Fragments arranged by size: 600 515 475 DrdI GACnn_nn'nnGTC Cuts at: 0 685 934 976 1590 Size: 685 249 42 614 Fragments arranged by size: 685 614 249 42 EaeI y'GGCC_r Cuts at: 0 329 1508 1590 Size: 329 1179 82 Fragments arranged by size: 1179 329 82 EagI C'GGCC_G Cuts at: 0 329 1590 Size: 329 1261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1464 1590 Size: 1464 126 EcoICRI GAG'CTC Cuts at: 0 22 1087 1590 Size: 22 1065 503 Fragments arranged by size: 1065 503 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 431 1590 Size: 37 394 1159 Fragments arranged by size: 1159 394 37 EcoO109I rG'GnC_Cy Cuts at: 0 1278 1590 Size: 1278 312 FspI TGC'GCA Cuts at: 0 1069 1590 Size: 1069 521 HaeII r_GCGC'y Cuts at: 0 108 166 315 522 665 861 946 1133 Size: 108 58 149 207 143 196 85 187 Cuts at: 1133 1181 1590 Size: 48 409 Fragments arranged by size: 409 207 196 187 149 143 108 85 58 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1280 1312 1590 Size: 1280 32 278 Fragments arranged by size: 1280 278 32 HincII GTy'rAC Cuts at: 0 1533 1590 Size: 1533 57 HindIII A'AGCT_T Cuts at: 0 1123 1590 Size: 1123 467 KasI G'GCGC_C Cuts at: 0 104 311 942 1129 1590 Size: 104 207 631 187 461 Fragments arranged by size: 631 461 207 187 104 MspA1I CmG'CkG Cuts at: 0 119 246 273 286 305 388 418 495 Size: 119 127 27 13 19 83 30 77 Cuts at: 495 674 709 716 1114 1590 Size: 179 35 7 398 476 Fragments arranged by size: 476 398 179 127 119 83 77 35 30 27 19 13 7 NarI GG'CG_CC Cuts at: 0 105 312 943 1130 1590 Size: 105 207 631 187 460 Fragments arranged by size: 631 460 207 187 105 NcoI C'CATG_G Cuts at: 0 1505 1590 Size: 1505 85 NspI r_CATG'y Cuts at: 0 598 657 1098 1590 Size: 598 59 441 492 Fragments arranged by size: 598 492 441 59 PasI CC'CwG_GG Cuts at: 0 316 1590 Size: 316 1274 PflMI CCAn_nnn'nTGG Cuts at: 0 454 1173 1260 1445 1506 1590 Size: 454 719 87 185 61 84 Fragments arranged by size: 719 454 185 87 84 61 PfoI T'CCnGG_A Cuts at: 0 218 1590 Size: 218 1372 PmlI CAC'GTG Cuts at: 0 515 1590 Size: 515 1075 PpuMI rG'GwC_Cy Cuts at: 0 1278 1590 Size: 1278 312 PstI C_TGCA'G Cuts at: 0 69 251 414 638 1220 1232 1250 1590 Size: 69 182 163 224 582 12 18 340 Fragments arranged by size: 582 340 224 182 163 69 18 12 PvuII CAG'CTG Cuts at: 0 305 495 1590 Size: 305 190 1095 Fragments arranged by size: 1095 305 190 RsrII CG'GwC_CG Cuts at: 0 1451 1590 Size: 1451 139 SacI G_AGCT'C Cuts at: 0 24 1089 1590 Size: 24 1065 501 Fragments arranged by size: 1065 501 24 SacII CC_GC'GG Cuts at: 0 419 710 1115 1590 Size: 419 291 405 475 Fragments arranged by size: 475 419 405 291 SbfI CC_TGCA'GG Cuts at: 0 638 1590 Size: 638 952 SexAI A'CCwGG_T Cuts at: 0 1259 1280 1590 Size: 1259 21 310 Fragments arranged by size: 1259 310 21 SfcI C'TryA_G Cuts at: 0 65 247 410 634 1057 1216 1228 1246 Size: 65 182 163 224 423 159 12 18 Cuts at: 1246 1590 Size: 344 Fragments arranged by size: 423 344 224 182 163 159 65 18 12 SfoI GGC'GCC Cuts at: 0 106 313 944 1131 1590 Size: 106 207 631 187 459 Fragments arranged by size: 631 459 207 187 106 SmaI CCC'GGG Cuts at: 0 32 1590 Size: 32 1558 SmlI C'TyrA_G Cuts at: 0 973 1590 Size: 973 617 SpeI A'CTAG_T Cuts at: 0 900 1590 Size: 900 690 SphI G_CATG'C Cuts at: 0 1098 1590 Size: 1098 492 StyI C'CwwG_G Cuts at: 0 1505 1590 Size: 1505 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 1590 Size: 265 1325 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 131 163 214 246 1322 1354 1590 Size: 131 32 51 32 1076 32 236 Fragments arranged by size: 1076 236 131 51 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1294 1590 Size: 1294 296 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 116 765 1404 1512 1590 Size: 116 649 639 108 78 Fragments arranged by size: 649 639 116 108 78 XmaI C'CCGG_G Cuts at: 0 30 1590 Size: 30 1560 XmnI GAAnn'nnTTC Cuts at: 0 148 1590 Size: 148 1442 Enzymes that do cut: AfeI AflIII AlfI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII HindIII KasI MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI PpuMI PstI PvuII RsrII SacI SacII SbfI SexAI SfcI SfoI SmaI SmlI SpeI SphI StyI TaqII TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BclI BfrBI BglII BmrI BmtI BplI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EarI EcoNI EcoRI EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SalI SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SrfI SspI StuI SwaI TatI XbaI XhoI ZraI