################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpmI BsaAI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BstBI BstUI Bsu36I Cfr10I CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT14I FbaI FseI FspI KpnI KspI MluI MroI MslI MspA1I MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 37 GGCGCC G[GA]^CG[CT]C AlwNI 883 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 21 GAGCAC G[AT]GC[AT]^C BamHI 591 GGATCC G^GATCC BbeI 37 GGCGCC GGCGC^C BglII 2196 AGATCT A^GATCT BlpI 1902 GCTTAGC GC^T[ACGT]AGC BmyI 21 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 1902 GCTTAGC GC^T[ACGT]AGC BsaBI 541 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 37 GGCGCC G[GA]^CG[CT]C BsiHKAI 21 GAGCAC G[AT]GC[AT]^C BsmBI 1432 CGTCTC CGTCTC(1/5) BsmFI 1488 GGGAC GGGAC(10/14) Bsp1286I 21 GAGCAC G[AGT]GC[ACT]^C BspDI 548 ATCGAT AT^CGAT BspMI 1666 ACCTGC ACCTGC(4/8) BssSI 734 CTCGTG CACGAG(-5/-1) Bst1107I 1446 GTATAC GTA^TAC BstAPI 1300 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 919 GGTTACC G^GT[ACGT]ACC BstNI 527 CCAGG CC^[AT]GG BstPI 919 GGTTACC G^GT[ACGT]ACC BstZ17I 1446 GTATAC GTA^TAC CelII 1902 GCTTAGC GC^T[ACGT]AGC ClaI 548 ATCGAT AT^CGAT DraIII 483 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 919 GGTTACC G^GT[ACGT]ACC EcoRII 527 CCAGG ^CC[AT]GG EcoT22I 1367 ATGCAT ATGCA^T FauI 111 GCGGG CCCGC(4/6) HgaI 926 GACGC GACGC(5/10) Hin1I 37 GGCGCC G[GA]^CG[CT]C HincII 579 GTTAAC GT[CT]^[GA]AC HindII 579 GTTAAC GT[CT]^[GA]AC HpaI 579 GTTAAC GTT^AAC KasI 37 GGCGCC G^GCGCC MamI 541 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1553 CAATTG C^AATTG MunI 1553 CAATTG C^AATTG MvaI 527 CCAGG CC^[AT]GG NarI 37 GGCGCC GG^CGCC NsiI 1367 ATGCAT ATGCA^T NspI 1704 GCATGT [GA]CATG^[CT] PaeR7I 1886 CTCGAG C^TCGAG PleI 1220 GACTC GAGTC(4/5) PshAI 908 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 37 GGCGCC GGC^GCC SmlI 1886 CTCGAG C^T[CT][GA]AG XhoI 1886 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1117 GTCTAC GT^[AC][GT]AC 1446 GTATAC AciI 7 GCGG CCGC(-3/-1) 111 GCGG AlwI 592 GATCC GGATC(4/5) 591 GGATC AvaII 27 GGACC G^G[AT]CC 1468 GGTCC BalI 557 TGGCCA TGG^CCA 979 TGGCCA BanI 37 GGCGCC G^G[CT][GA]CC 1663 GGCACC BbsI 1240 GAAGAC GAAGAC(2/6) 1864 GAAGAC BcnI 683 CCCGG CC^[GC]GG 725 CCCGG BpuAI 1240 GAAGAC GAAGAC(2/6) 1864 GAAGAC BsiYI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1311 CCTCAAAGTGG BslI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1311 CCTCAAAGTGG BstXI 462 CCATGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1811 CCAAACTGCTGG BstYI 591 GGATCC [GA]^GATC[CT] 2196 AGATCT DraI 627 TTTAAA TTT^AAA 2046 TTTAAA EaeI 557 TGGCCA [CT]^GGCC[GA] 979 TGGCCA EarI 72 CTCTTC CTCTTC(1/4) 2073 GAAGAG HaeII 37 GGCGCC [GA]GCGC^[CT] 96 GGCGCT HindIII 145 AAGCTT A^AGCTT 1839 AAGCTT Ksp632I 72 CTCTTC CTCTTC(1/4) 2073 GAAGAG MaeII 763 ACGT A^CGT 1927 ACGT MflI 591 GGATCC [GA]^GATC[CT] 2196 AGATCT MluNI 557 TGGCCA TGG^CCA 979 TGGCCA MscI 557 TGGCCA TGG^CCA 979 TGGCCA NciI 683 CCCGG CC^[GC]GG 725 CCCGG PstI 298 CTGCAG CTGCA^G 1747 CTGCAG SfcI 298 CTGCAG C^T[GA][CT]AG 1747 CTGCAG TaqI 549 TCGA T^CGA 1887 TCGA TspRI 894 CACTG CA[GC]TG[ACGT][ACGT]^ 1382 CACTG TthHB8I 549 TCGA T^CGA 1887 TCGA XhoII 591 GGATCC [GA]^GATC[CT] 2196 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I AvaI BbvI BfaI BseRI BsmAI BsmI BsoBI BspHI BsrDI BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DpnII EcoRI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MwoI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I XcmI XmnI