(Circular) (Six-base) MAPSORT of: pFN21AE2084.seq Check: 1510 from: 1 to: 6982 pFN21AE2084 6982 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3680 3680 Size: 6982 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4792 278 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3123 3452 4325 1937 Size: 1186 329 873 4594 Fragments arranged by size: 4594 1186 873 329 Acc65I G'GTAC_C Cuts at: 4303 4303 Size: 6982 AclI AA'CG_TT Cuts at: 1563 5109 5482 1563 Size: 3546 373 3063 Fragments arranged by size: 3546 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 5045 6429 6697 1632 Size: 494 2919 1384 268 1917 Fragments arranged by size: 2919 1917 1384 494 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6785 AflIII A'CryG_T Cuts at: 1203 5881 6858 1203 Size: 4678 977 1327 Fragments arranged by size: 4678 1327 977 AgeI A'CCGG_T Cuts at: 5813 5813 Size: 6982 AhdI GACnn_n'nnGTC Cuts at: 5711 5711 Size: 6982 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6982 AlwNI CAG_nnn'CTG Cuts at: 2893 4725 6297 2893 Size: 1832 1572 3578 Fragments arranged by size: 3578 1832 1572 ApaLI G'TGCA_C Cuts at: 5039 6195 5039 Size: 1156 5826 ApoI r'AATT_y Cuts at: 1493 2346 2421 2660 2734 2818 3059 3289 3518 Size: 853 75 239 74 84 241 230 229 Cuts at: 3518 4066 4211 4291 4517 4669 6778 1493 Size: 548 145 80 226 152 2109 1697 Fragments arranged by size: 2109 1697 853 548 241 239 230 229 226 152 145 84 80 75 74 AseI AT'TA_AT Cuts at: 160 1032 5536 160 Size: 872 4504 1606 Fragments arranged by size: 4504 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2038 3891 4307 1870 Size: 73 80 15 1853 416 4545 Fragments arranged by size: 4545 1853 416 80 73 15 BamHI G'GATC_C Cuts at: 2596 4312 4679 2596 Size: 1716 367 4899 Fragments arranged by size: 4899 1716 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3668 4303 5758 618 Size: 325 217 627 255 1626 635 1455 1842 Fragments arranged by size: 1842 1626 1455 635 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 4301 1391 Size: 2910 4072 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 6982 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3252 3876 928 Size: 971 1353 624 4034 Fragments arranged by size: 4034 1353 971 624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5173 5207 1903 Size: 34 3236 34 3678 Fragments arranged by size: 3678 3236 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4874 6084 4874 Size: 1210 5772 BclI T'GATC_A Cuts at: 1548 1548 Size: 6982 BfrBI ATG'CAT Cuts at: 3374 3374 Size: 6982 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5593 136 Size: 107 122 71 631 605 337 3584 1525 Fragments arranged by size: 3584 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4201 6977 4201 Size: 2776 4206 BlpI GC'TnA_GC Cuts at: 3908 4399 3908 Size: 491 6491 Bme1580I G_kGCm'C Cuts at: 1790 5043 6199 1790 Size: 3253 1156 2573 Fragments arranged by size: 3253 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6982 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 4713 5671 Size: 416 53 83 399 24 1449 1847 958 Cuts at: 5671 442 Size: 1753 Fragments arranged by size: 1847 1753 1449 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6982 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 6982 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5642 1239 Size: 4403 2579 Bpu10I CC'TnA_GC Cuts at: 2709 3703 2709 Size: 994 5988 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5107 5972 6270 5107 Size: 865 298 5819 Fragments arranged by size: 5819 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5645 882 Size: 617 4146 2219 Fragments arranged by size: 4146 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4773 493 Size: 711 3569 2702 Fragments arranged by size: 3569 2702 711 BsaBI GATnn'nnATC Cuts at: 2550 6976 2550 Size: 4426 2556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 4789 5171 5806 Size: 53 83 186 936 510 2746 382 635 Cuts at: 5806 275 Size: 1451 Fragments arranged by size: 2746 1451 936 635 510 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5415 5813 6087 6234 6645 5415 Size: 398 274 147 411 5752 Fragments arranged by size: 5752 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3922 3952 1178 Size: 30 165 30 2519 30 4208 Fragments arranged by size: 4208 2519 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2041 2044 2144 2606 1186 Size: 852 3 3 100 462 5562 Fragments arranged by size: 5562 852 462 100 3 3 BseYI C'CCAG_C Cuts at: 6185 6681 6185 Size: 496 6486 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6409 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4432 5193 6221 1540 Size: 275 186 2431 761 1028 2301 Fragments arranged by size: 2431 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 5043 5128 6199 2030 Size: 3013 85 1071 2813 Fragments arranged by size: 3013 2813 1071 85 BsmI GAATG_Cn' Cuts at: 2489 2527 3374 4493 4586 2489 Size: 38 847 1119 93 4885 Fragments arranged by size: 4885 1119 847 93 38 BsmBI CGTCTCn'nnnn_ Cuts at: 3443 3443 Size: 6982 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 4301 5043 5128 6199 1391 Size: 399 240 2271 742 85 1071 2174 Fragments arranged by size: 2271 2174 1071 742 399 240 85 BspEI T'CCGG_A Cuts at: 6645 6645 Size: 6982 BspHI T'CATG_A Cuts at: 3285 3343 3865 4871 6601 3285 Size: 58 522 1006 1730 3666 Fragments arranged by size: 3666 1730 1006 522 58 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 4337 844 Size: 2836 657 3489 Fragments arranged by size: 3489 2836 657 BsrBI CCG'CTC Cuts at: 1394 1823 4869 1394 Size: 429 3046 3507 Fragments arranged by size: 3507 3046 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2699 3649 3837 5477 5651 65 Size: 1183 1451 950 188 1640 174 1396 Fragments arranged by size: 1640 1451 1396 1183 950 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5626 5813 1700 Size: 110 3816 187 2869 Fragments arranged by size: 3816 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6982 BssSI C'ACGA_G Cuts at: 2739 5042 6054 2739 Size: 2303 1012 3667 Fragments arranged by size: 3667 2303 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3311 794 Size: 2517 4465 BstBI TT'CG_AA Cuts at: 5786 5786 Size: 6982 BstEII G'GTnAC_C Cuts at: 1184 2924 1184 Size: 1740 5242 BstXI CCAn_nnnn'nTGG Cuts at: 2474 3823 2474 Size: 1349 5633 BstYI r'GATC_y Cuts at: 1972 2596 4201 4312 4679 5064 5081 6533 6619 Size: 624 1605 111 367 385 17 1452 86 Cuts at: 6619 6631 6977 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1605 1452 624 385 367 346 111 86 17 12 BstZ17I GTA'TAC Cuts at: 3453 3453 Size: 6982 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6430 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6243 Fragments arranged by size: 6243 683 56 BtsI GCAGTG_nn' Cuts at: 4498 5292 5312 4498 Size: 794 20 6168 Fragments arranged by size: 6168 794 20 ClaI AT'CG_AT Cuts at: 1554 2554 1554 Size: 1000 5982 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6947 DraI TTT'AAA Cuts at: 2634 4053 4286 2634 Size: 1419 233 5330 Fragments arranged by size: 5330 1419 233 DraIII CAC_nnn'GTG Cuts at: 2493 2493 Size: 6982 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5989 809 Size: 507 4673 1802 Fragments arranged by size: 4673 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2562 2984 4429 Size: 54 1425 50 275 18 732 422 1445 Cuts at: 4429 5318 6947 8 Size: 889 1629 43 Fragments arranged by size: 1629 1445 1425 889 732 422 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4429 1537 Size: 275 2617 4090 Fragments arranged by size: 4090 2617 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2083 4073 4912 1353 Size: 730 1990 839 3423 Fragments arranged by size: 3423 1990 839 730 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5569 5943 6089 387 Size: 1064 138 3980 374 146 1280 Fragments arranged by size: 3980 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 5045 5642 6429 6697 1239 Size: 393 494 2919 597 787 268 1524 Fragments arranged by size: 2919 1524 787 597 494 393 268 EcoRI G'AATT_C Cuts at: 2346 2660 2734 3289 4291 4669 2346 Size: 314 74 555 1002 378 4659 Fragments arranged by size: 4659 1002 555 378 314 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 6982 FspI TGC'GCA Cuts at: 5488 5488 Size: 6982 HaeII r_GCGC'y Cuts at: 1678 2046 2105 6129 1678 Size: 368 59 4024 2531 Fragments arranged by size: 4024 2531 368 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3847 3879 3997 4029 5623 Size: 32 135 32 2275 32 118 32 1594 Cuts at: 5623 5655 5697 5729 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2275 1594 135 118 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 4326 4572 669 Size: 988 281 648 1740 246 3079 Fragments arranged by size: 3079 1740 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 2150 3844 748 Size: 1402 1694 3886 Fragments arranged by size: 3886 1694 1402 HpaI GTT'AAC Cuts at: 2586 4572 2586 Size: 1986 4996 KasI G'GCGC_C Cuts at: 2042 2042 Size: 6982 KpnI G_GTAC'C Cuts at: 4307 4307 Size: 6982 MfeI C'AATT_G Cuts at: 3558 4581 3558 Size: 1023 5959 MluI A'CGCG_T Cuts at: 6858 6858 Size: 6982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3222 6096 6280 1305 Size: 1917 2874 184 2007 Fragments arranged by size: 2874 2007 1917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 2986 10 Size: 54 1425 343 732 422 4006 Fragments arranged by size: 4006 1425 732 422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4940 5299 5458 518 Size: 648 3774 359 159 2042 Fragments arranged by size: 3774 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4398 5075 6223 6468 1606 Size: 2792 677 1148 245 2120 Fragments arranged by size: 2792 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2043 2043 Size: 6982 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6430 NdeI CA'TA_TG Cuts at: 387 387 Size: 6982 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6982 NotI GC'GGCC_GC Cuts at: 4429 4429 Size: 6982 NsiI A_TGCA'T Cuts at: 3376 3376 Size: 6982 NspI r_CATG'y Cuts at: 1132 1229 3713 4340 5885 1132 Size: 97 2484 627 1545 2229 Fragments arranged by size: 2484 2229 1545 627 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6982 PciI A'CATG_T Cuts at: 5881 5881 Size: 6982 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6982 PmeI GTTT'AAAC Cuts at: 4286 4286 Size: 6982 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5051 5083 6564 6596 5051 Size: 32 1481 32 5437 Fragments arranged by size: 5437 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2917 1613 Size: 1304 5678 PsiI TTA'TAA Cuts at: 4552 4552 Size: 6982 PspXI vC'TCGA_Gb Cuts at: 3891 3891 Size: 6982 PstI C_TGCA'G Cuts at: 830 2307 3756 4334 830 Size: 1477 1449 578 3478 Fragments arranged by size: 3478 1477 1449 578 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6982 SalI G'TCGA_C Cuts at: 1936 4324 1936 Size: 2388 4594 SbfI CC_TGCA'GG Cuts at: 4334 4334 Size: 6982 ScaI AGT'ACT Cuts at: 5230 6942 5230 Size: 1712 5270 SfcI C'TryA_G Cuts at: 826 1045 2303 3752 4330 5465 6146 6337 826 Size: 219 1258 1449 578 1135 681 191 1471 Fragments arranged by size: 1471 1449 1258 1135 681 578 219 191 SfoI GGC'GCC Cuts at: 2044 2044 Size: 6982 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6982 SmaI CCC'GGG Cuts at: 1872 4309 1872 Size: 2437 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3891 5086 5987 6249 820 Size: 197 926 1948 1195 901 262 1553 Fragments arranged by size: 1948 1553 1195 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4773 493 Size: 4280 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6982 SphI G_CATG'C Cuts at: 1132 4340 1132 Size: 3208 3774 SspI AAT'ATT Cuts at: 5 52 4906 5 Size: 47 4854 2081 Fragments arranged by size: 4854 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6982 StyI C'CwwG_G Cuts at: 513 1065 4421 513 Size: 552 3356 3074 Fragments arranged by size: 3356 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5009 5026 5179 5364 5809 325 Size: 1255 3429 17 153 185 445 1498 Fragments arranged by size: 3429 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5228 6940 Size: 274 80 33 51 1443 2180 1071 1712 Cuts at: 6940 96 Size: 138 Fragments arranged by size: 2180 1712 1443 1071 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2725 2757 6942 6974 2725 Size: 32 4185 32 2733 Fragments arranged by size: 4185 2733 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6982 XbaI T'CTAG_A Cuts at: 4318 4318 Size: 6982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3466 3662 3823 3466 Size: 196 161 6625 Fragments arranged by size: 6625 196 161 XhoI C'TCGA_G Cuts at: 1943 3891 1943 Size: 1948 5034 XmaI C'CCGG_G Cuts at: 1870 4307 1870 Size: 2437 4545 XmnI GAAnn'nnTTC Cuts at: 2153 2346 2754 5111 2153 Size: 193 408 2357 4024 Fragments arranged by size: 4024 2357 408 193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4790 276 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI