Restriction map for
pFN21AE4047
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MamI MfeI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 406 GACGCC G[GA]^CG[CT]C
AflIII 161 ACGCGT A^C[GA][CT]GT
AhdI 406 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 728 GATCC GGATC(4/5)
ApaI 496 GGGCCC GGGCC^C
AspEI 406 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 193 GTCTTC GAAGAC(2/6)
BcgI 504 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 193 GTCTTC GAAGAC(2/6)
BsaHI 406 GACGCC G[GA]^CG[CT]C
BsaI 607 GGTCTC GGTCTC(1/5)
BsmFI 425 GGGAC GGGAC(10/14)
BssSI 532 CTCGTG CACGAG(-5/-1)
BstYI 727 AGATCC [GA]^GATC[CT]
Eam1105I 406 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 221 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 59 GGCCGGCC GGCCGG^CC
HgaI 406 GACGC GACGC(5/10)
Hin1I 406 GACGCC G[GA]^CG[CT]C
MaeII 191 ACGT A^CGT
MboII 194 TCTTC GAAGA(8/7)
MflI 727 AGATCC [GA]^GATC[CT]
MluI 161 ACGCGT A^CGCGT
NspI 125 ACATGC [GA]CATG^[CT]
PpuMI 425 GGGACCT [GA]G^G[AT]CC[CT]
SbfI 759 CCTGCAGG CCTGCA^GG
SfaNI 257 GCATC GCATC(5/9)
SfiI 477 GGCCTACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 91 CCCGGG CCC^GGG
Sse8387I 759 CCTGCAGG CCTGCA^GG
StuI 506 AGGCCT AGG^CCT
XcmI 333 CCACGCTGTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 727 AGATCC [GA]^GATC[CT]
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 583 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
754 CAGCGCCTG
AspHI 246 GAGCAC G[AT]GC[AT]^C
362 GTGCTC
BalI 350 TGGCCA TGG^CCA
579 TGGCCA
BanI 230 GGCACC G^G[CT][GA]CC
328 GGCACC
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
362 GTGCTC
BsmAI 608 GTCTC GTCTC(1/5)
792 GTCTC
BsrBI 180 CCGCTC CCGCTC(-3/-3)
735 CCGCTC
BsrI 308 ACTGG ACTGG(1/-1)
582 CCAGT
DdeI 562 CTCAG C^T[ACGT]AG
788 CTCAG
DpnII 303 GATC ^GATC
728 GATC
DraII 425 GGGACCT [GA]G^G[ACGT]CC[CT]
495 GGGGCCC
DraIII 232 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
373 CACTCCGTG
EagI 58 CGGCCG C^GGCCG
97 CGGCCG
EclXI 58 CGGCCG C^GGCCG
97 CGGCCG
Eco52I 58 CGGCCG C^GGCCG
97 CGGCCG
EcoO109I 425 GGGACCT [GA]G^G[ACGT]CC[CT]
495 GGGGCCC
HinfI 412 GAGTC G^A[ACGT]TC
523 GACTC
KspI 261 CCGCGG CCGC^GG
282 CCGCGG
MaeIII 404 GTGAC ^GT[ACGT]AC
664 GTCAC
MboI 303 GATC ^GATC
728 GATC
MluNI 350 TGGCCA TGG^CCA
579 TGGCCA
MscI 350 TGGCCA TGG^CCA
579 TGGCCA
NdeII 303 GATC ^GATC
728 GATC
PflMI 114 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGTACCTGG
PleI 523 GACTC GAGTC(4/5)
412 GAGTC
PstI 760 CTGCAG CTGCA^G
808 CTGCAG
PvuII 115 CAGCTG CAG^CTG
151 CAGCTG
SacII 261 CCGCGG CCGC^GG
282 CCGCGG
Sau3AI 303 GATC ^GATC
728 GATC
SmlI 25 CTCAAG C^T[CT][GA]AG
610 CTCAAG
Tsp45I 404 GTGAC ^GT[GC]AC
664 GTCAC
Van91I 114 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BsaJI BsgI BsiEI BsiYI
BslI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV RsaI Sau96I ScrFI
SfcI TseI TspRI