(Circular) (Six-base) MAPSORT of: pFN21AE4047.seq Check: 4953 from: 1 to: 5524 pFN21AE4047 5524 bp With 182 enzymes: SgfI * March 28, 2012 13:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2845 2845 Size: 5524 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4971 5239 1632 Size: 1955 1384 268 1917 Fragments arranged by size: 1955 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 2166 4423 5400 1203 Size: 963 2257 977 1327 Fragments arranged by size: 2257 1327 977 963 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 2416 4253 2416 Size: 1837 3687 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 2593 2764 3267 4839 2593 Size: 171 503 1572 3278 Fragments arranged by size: 3278 1572 503 171 ApaI G_GGCC'C Cuts at: 2505 2505 Size: 5524 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2096 2177 2699 2849 1870 Size: 73 77 76 81 522 150 4545 Fragments arranged by size: 4545 522 150 81 77 76 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 2333 2845 4300 618 Size: 325 217 627 448 98 512 1455 1842 Fragments arranged by size: 1842 1455 627 512 448 325 217 98 BanII G_rGCy'C Cuts at: 1391 2097 2505 2706 2843 1391 Size: 706 408 201 137 4072 Fragments arranged by size: 4072 706 408 201 137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2191 928 Size: 971 292 4261 Fragments arranged by size: 4261 971 292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2498 2532 3715 3749 1903 Size: 34 561 34 1183 34 3678 Fragments arranged by size: 3678 1183 561 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2101 2110 2323 Size: 107 122 71 631 605 429 9 213 Cuts at: 2323 2489 4135 136 Size: 166 1646 1525 Fragments arranged by size: 1646 1525 631 605 429 213 166 122 107 71 9 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 2505 2564 3585 4741 1790 Size: 715 59 1021 1156 2573 Fragments arranged by size: 2573 1156 1021 715 59 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4184 1239 Size: 2945 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2600 3649 4514 4812 2015 Size: 585 1049 865 298 2727 Fragments arranged by size: 2727 1049 865 585 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2618 4187 882 Size: 617 1119 1569 2219 Fragments arranged by size: 2219 1569 1119 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2412 3331 3713 4348 Size: 53 83 186 936 879 919 382 635 Cuts at: 4348 275 Size: 1451 Fragments arranged by size: 1451 936 919 879 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2762 2792 1178 Size: 30 165 30 1359 30 3910 Fragments arranged by size: 3910 1359 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5524 BseYI C'CCAG_C Cuts at: 2427 4727 5223 2427 Size: 2300 496 2728 Fragments arranged by size: 2728 2300 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2109 2174 2279 2360 1163 Size: 573 373 65 105 81 4327 Fragments arranged by size: 4327 573 373 105 81 65 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 2105 2218 2974 3735 4763 Size: 275 186 65 39 113 756 761 1028 Cuts at: 4763 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 756 275 186 113 65 39 BsiHKAI G_wGCw'C Cuts at: 2255 2371 3585 3670 4741 2255 Size: 116 1214 85 1071 3038 Fragments arranged by size: 3038 1214 1071 116 85 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2255 2371 2505 2564 2706 2843 Size: 399 307 158 116 134 59 142 137 Cuts at: 2843 3585 3670 4741 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 307 158 142 137 134 116 85 59 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2742 3411 1394 Size: 429 364 555 669 3507 Fragments arranged by size: 3507 669 555 429 364 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 2065 2104 2217 2317 4168 4355 Size: 110 243 12 39 113 100 1851 187 Cuts at: 4355 1700 Size: 2869 Fragments arranged by size: 2869 1851 243 187 113 110 100 39 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 2537 3584 4596 2537 Size: 1047 1012 3465 Fragments arranged by size: 3465 1047 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2732 2854 3221 3606 3623 5075 5161 5173 Size: 760 122 367 385 17 1452 86 12 Cuts at: 5173 5519 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 2266 2287 2358 2382 513 Size: 552 939 237 25 21 71 24 3655 Fragments arranged by size: 3655 939 552 237 71 25 24 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 2413 3040 3834 3854 2413 Size: 627 794 20 4083 Fragments arranged by size: 4083 794 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DraIII CAC_nnn'GTG Cuts at: 2242 2383 2242 Size: 141 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2067 2102 Size: 54 1425 50 275 18 233 4 35 Cuts at: 2102 2315 2355 2584 2971 3860 5489 8 Size: 213 40 229 387 889 1629 43 Fragments arranged by size: 1629 1425 889 387 275 233 229 213 54 50 43 40 35 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2063 2102 2971 1537 Size: 275 251 39 869 4090 Fragments arranged by size: 4090 869 275 251 39 EarI CTCTTCn'nnn_ Cuts at: 1353 3454 1353 Size: 2101 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3587 4184 4971 5239 1239 Size: 393 1955 597 787 268 1524 Fragments arranged by size: 1955 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2230 2230 Size: 5524 EcoO109I rG'GnC_Cy Cuts at: 2431 2501 2431 Size: 70 5454 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FseI GG_CCGG'CC Cuts at: 2069 2069 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2060 2399 2764 4671 1678 Size: 382 339 365 1907 2531 Fragments arranged by size: 2531 1907 382 365 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2762 2794 4165 Size: 32 135 32 830 32 328 32 1371 Cuts at: 4165 4197 4239 4271 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1371 830 328 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KpnI G_GTAC'C Cuts at: 2849 2849 Size: 5524 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 2166 5400 2166 Size: 3234 2290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 4638 4822 1305 Size: 801 2532 184 2007 Fragments arranged by size: 2532 2007 801 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2357 2586 10 Size: 54 1425 343 525 229 2948 Fragments arranged by size: 2948 1425 525 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 3482 3841 4000 518 Size: 648 2316 359 159 2042 Fragments arranged by size: 2316 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2138 2158 2268 2289 2940 3617 4765 Size: 516 16 20 110 21 651 677 1148 Cuts at: 4765 5010 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 651 516 245 110 21 20 16 NaeI GCC'GGC Cuts at: 2055 2067 2106 2319 2055 Size: 12 39 213 5260 Fragments arranged by size: 5260 213 39 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4033 Fragments arranged by size: 4033 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NgoMIV G'CCGG_C Cuts at: 2053 2065 2104 2317 2053 Size: 12 39 213 5260 Fragments arranged by size: 5260 213 39 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2134 2882 4427 1132 Size: 97 905 748 1545 2229 Fragments arranged by size: 2229 1545 905 748 97 PasI CC'CwG_GG Cuts at: 1348 2749 1348 Size: 1401 4123 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2125 2593 1108 Size: 1017 468 4039 Fragments arranged by size: 4039 1017 468 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2431 Size: 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PspOMI G'GGCC_C Cuts at: 2501 2501 Size: 5524 PstI C_TGCA'G Cuts at: 830 2769 2817 2876 830 Size: 1939 48 59 3478 Fragments arranged by size: 3478 1939 59 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 PvuII CAG'CTG Cuts at: 2122 2158 2122 Size: 36 5488 SacII CC_GC'GG Cuts at: 2269 2290 2269 Size: 21 5503 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2769 2876 2769 Size: 107 5417 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SexAI A'CCwGG_T Cuts at: 2820 2820 Size: 5524 SfcI C'TryA_G Cuts at: 826 1045 2343 2765 2813 2872 4007 4688 4879 Size: 219 1298 422 48 59 1135 681 191 Cuts at: 4879 826 Size: 1471 Fragments arranged by size: 1471 1298 1135 681 422 219 191 59 48 SfiI GGCCn_nnn'nGGCC Cuts at: 2489 2489 Size: 5524 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2098 2851 1872 Size: 226 753 4545 Fragments arranged by size: 4545 753 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 2615 3628 4529 4791 820 Size: 197 926 87 585 1013 901 262 1553 Fragments arranged by size: 1553 1013 926 901 585 262 197 87 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SrfI GCCC'GGGC Cuts at: 2098 2098 Size: 5524 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 2513 1517 Size: 996 4528 StyI C'CwwG_G Cuts at: 513 1065 2004 2963 513 Size: 552 939 959 3074 Fragments arranged by size: 3074 959 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 3551 3568 3721 3906 4351 325 Size: 1255 771 1200 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3770 5482 96 Size: 274 80 33 51 1443 1793 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2345 2345 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2096 2849 1870 Size: 226 753 4545 Fragments arranged by size: 4545 753 226 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SfoI SwaI