(Linear) (Six-base) MAPSORT of: KIEE4047.seq Check: 2141 from: 1 to: 819 KIEE4047.orf With 182 enzymes: SgfI * March 22, 2012 17:15 .. AflIII A'CryG_T Cuts at: 0 161 819 Size: 161 658 AhdI GACnn_n'nnGTC Cuts at: 0 411 819 Size: 411 408 AlwNI CAG_nnn'CTG Cuts at: 0 588 759 819 Size: 588 171 60 Fragments arranged by size: 588 171 60 ApaI G_GGCC'C Cuts at: 0 500 819 Size: 500 319 AvaI C'yCGr_G Cuts at: 0 15 91 172 694 819 Size: 15 76 81 522 125 Fragments arranged by size: 522 125 81 76 15 BanI G'GyrC_C Cuts at: 0 230 328 819 Size: 230 98 491 Fragments arranged by size: 491 230 98 BanII G_rGCy'C Cuts at: 0 92 500 701 819 Size: 92 408 201 118 Fragments arranged by size: 408 201 118 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 186 819 Size: 186 633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 527 819 Size: 493 34 292 Fragments arranged by size: 493 292 34 BglI GCCn_nnn'nGGC Cuts at: 0 96 105 318 484 819 Size: 96 9 213 166 335 Fragments arranged by size: 335 213 166 96 9 Bme1580I G_kGCm'C Cuts at: 0 500 559 819 Size: 500 59 260 Fragments arranged by size: 500 260 59 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 198 819 Size: 198 621 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 595 819 Size: 10 585 224 Fragments arranged by size: 585 224 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 613 819 Size: 613 206 BsaHI Gr'CG_yC Cuts at: 0 407 819 Size: 407 412 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 757 787 819 Size: 757 30 32 Fragments arranged by size: 757 32 30 BseYI C'CCAG_C Cuts at: 0 422 819 Size: 422 397 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 169 274 355 819 Size: 104 65 105 81 464 Fragments arranged by size: 464 105 104 81 65 BsiEI CG_ry'CG Cuts at: 0 61 100 213 819 Size: 61 39 113 606 Fragments arranged by size: 606 113 61 39 BsiHKAI G_wGCw'C Cuts at: 0 250 366 819 Size: 250 116 453 Fragments arranged by size: 453 250 116 Bsp1286I G_dGCh'C Cuts at: 0 92 250 366 500 559 701 819 Size: 92 158 116 134 59 142 118 Fragments arranged by size: 158 142 134 118 116 92 59 BsrBI CCG'CTC Cuts at: 0 182 737 819 Size: 182 555 82 Fragments arranged by size: 555 182 82 BsrFI r'CCGG_y Cuts at: 0 48 60 99 212 312 819 Size: 48 12 39 113 100 507 Fragments arranged by size: 507 113 100 48 39 12 BssSI C'ACGA_G Cuts at: 0 532 819 Size: 532 287 BstYI r'GATC_y Cuts at: 0 727 819 Size: 727 92 BtgI C'CryG_G Cuts at: 0 236 261 282 353 377 819 Size: 236 25 21 71 24 442 Fragments arranged by size: 442 236 71 25 24 21 BtsI GCAGTG_nn' Cuts at: 0 408 819 Size: 408 411 DraIII CAC_nnn'GTG Cuts at: 0 237 378 819 Size: 237 141 441 Fragments arranged by size: 441 237 141 EaeI y'GGCC_r Cuts at: 0 58 62 97 310 350 579 819 Size: 58 4 35 213 40 229 240 Fragments arranged by size: 240 229 213 58 40 35 4 EagI C'GGCC_G Cuts at: 0 58 97 819 Size: 58 39 722 Fragments arranged by size: 722 58 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 225 819 Size: 225 594 EcoO109I rG'GnC_Cy Cuts at: 0 426 496 819 Size: 426 70 323 Fragments arranged by size: 426 323 70 FseI GG_CCGG'CC Cuts at: 0 64 819 Size: 64 755 HaeII r_GCGC'y Cuts at: 0 55 394 759 819 Size: 55 339 365 60 Fragments arranged by size: 365 339 60 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 397 429 757 789 819 Size: 397 32 328 32 30 Fragments arranged by size: 397 328 32 32 30 MluI A'CGCG_T Cuts at: 0 161 819 Size: 161 658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 101 819 Size: 101 718 MscI TGG'CCA Cuts at: 0 352 581 819 Size: 352 229 238 Fragments arranged by size: 352 238 229 MspA1I CmG'CkG Cuts at: 0 117 133 153 263 284 819 Size: 117 16 20 110 21 535 Fragments arranged by size: 535 117 110 21 20 16 NaeI GCC'GGC Cuts at: 0 50 62 101 314 819 Size: 50 12 39 213 505 Fragments arranged by size: 505 213 50 39 12 NgoMIV G'CCGG_C Cuts at: 0 48 60 99 312 819 Size: 48 12 39 213 507 Fragments arranged by size: 507 213 48 39 12 NspI r_CATG'y Cuts at: 0 129 819 Size: 129 690 PasI CC'CwG_GG Cuts at: 0 744 819 Size: 744 75 PflMI CCAn_nnn'nTGG Cuts at: 0 120 588 819 Size: 120 468 231 Fragments arranged by size: 468 231 120 PpuMI rG'GwC_Cy Cuts at: 0 426 819 Size: 426 393 PspOMI G'GGCC_C Cuts at: 0 496 819 Size: 496 323 PstI C_TGCA'G Cuts at: 0 764 812 819 Size: 764 48 7 Fragments arranged by size: 764 48 7 PvuII CAG'CTG Cuts at: 0 117 153 819 Size: 117 36 666 Fragments arranged by size: 666 117 36 SacII CC_GC'GG Cuts at: 0 264 285 819 Size: 264 21 534 Fragments arranged by size: 534 264 21 SbfI CC_TGCA'GG Cuts at: 0 764 819 Size: 764 55 SfcI C'TryA_G Cuts at: 0 338 760 808 819 Size: 338 422 48 11 Fragments arranged by size: 422 338 48 11 SfiI GGCCn_nnn'nGGCC Cuts at: 0 484 819 Size: 484 335 SmaI CCC'GGG Cuts at: 0 93 819 Size: 93 726 SmlI C'TyrA_G Cuts at: 0 25 610 819 Size: 25 585 209 Fragments arranged by size: 585 209 25 SrfI GCCC'GGGC Cuts at: 0 93 819 Size: 93 726 StuI AGG'CCT Cuts at: 0 508 819 Size: 508 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 346 819 Size: 346 473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 340 819 Size: 340 479 XmaI C'CCGG_G Cuts at: 0 91 819 Size: 91 728 Enzymes that do cut: AflIII AhdI AlwNI ApaI AvaI BanI BanII BbsI BcgI BglI Bme1580I BplI BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstYI BtgI BtsI DraIII EaeI EagI EcoNI EcoO109I FseI HaeII Hin4I MluI MmeI MscI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII SacII SbfI SfcI SfiI SmaI SmlI SrfI StuI TaqII XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BsaAI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI