################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DrdI DsaI EagI EclXI Eco52I EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AhdI 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1528 GTGCAC G^TGCAC AlwNI 1831 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1528 GTGCAC G^TGCAC AspEI 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 616 CCCGAG C^[CT]CG[GA]G BcnI 698 CCGGG CC^[GC]GG BfaI 1201 CTAG C^TAG BglII 863 AGATCT A^GATCT BsaBI 864 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 573 CGACCG CG[GA][CT]^CG BsmBI 637 CGTCTC CGTCTC(1/5) BsoBI 616 CCCGAG C^[CT]CG[GA]G BspLU11I 1870 ACATGT A^CATGT BsrFI 449 GCCGGC [GA]^CCGG[CT] BstEII 534 GGTCACC G^GT[ACGT]ACC BstPI 534 GGTCACC G^GT[ACGT]ACC BstXI 1337 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 449 GCCGGC [GA]^CCGG[CT] DraIII 415 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 708 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 534 GGTCACC G^GT[ACGT]ACC EcoRV 2074 GATATC GAT^ATC FauI 1690 CCCGC CCCGC(4/6) MaeI 1201 CTAG C^TAG MamI 864 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 449 GCCGGC GCC^GGC NciI 698 CCGGG CC^[GC]GG NgoMIV 449 GCCGGC G^CCGGC PflFI 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1830 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 1464 GCTCTTC GCTCTTC(1/4) ScaI 899 AGTACT AGT^ACT SexAI 416 ACCTGGT A^CC[AT]GGT SphI 587 GCATGC GCATG^C TfiI 1197 GAATC G^A[AT]TC Tth111I 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1830 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1200 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AcyI 635 GGCGTC G[GA]^CG[CT]C 982 GACGCC AflIII 1870 ACATGT A^C[GA][CT]GT 2024 ACGTGT AlwI 217 GATCC GGATC(4/5) 1341 GATCC AocI 321 CCTCAGG CC^T[ACGT]AGG 1496 CCTCAGG Aor51HI 647 AGCGCT AGC^GCT 746 AGCGCT ApaI 700 GGGCCC GGGCC^C 1477 GGGCCC Asp700I 543 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2160 GAAGCACTTC AvaII 1350 GGACC G^G[AT]CC 2056 GGTCC AviII 356 TGCGCA TGC^GCA 1148 TGCGCA BbsI 1803 GAAGAC GAAGAC(2/6) 1882 GAAGAC BpuAI 1803 GAAGAC GAAGAC(2/6) 1882 GAAGAC BsaAI 1420 TACGTG [CT]AC^GT[GA] 2023 TACGTG BsaHI 635 GGCGTC G[GA]^CG[CT]C 982 GACGCC BsmAI 1306 GAGAC GTCTC(1/5) 638 GTCTC BsmFI 1502 GGGAC GGGAC(10/14) 1970 GGGAC BsmI 1154 GCATTC GAATGC(1/-1) 1321 GCATTC Bsp1407I 34 TGTACA T^GTACA 1749 TGTACA BsrDI 908 GCAATG GCAATG(2/0) 2064 GCAATG BsrGI 34 TGTACA T^GTACA 1749 TGTACA BstYI 863 AGATCT [GA]^GATC[CT] 1340 AGATCC Bsu36I 321 CCTCAGG CC^T[ACGT]AGG 1496 CCTCAGG DraI 1093 TTTAAA TTT^AAA 2029 TTTAAA DraII 1476 AGGGCCC [GA]G^G[ACGT]CC[CT] 2126 GGGGCCT EarI 1009 CTCTTC CTCTTC(1/4) 1465 CTCTTC Eco47III 647 AGCGCT AGC^GCT 746 AGCGCT Eco81I 321 CCTCAGG CC^T[ACGT]AGG 1496 CCTCAGG EcoO109I 1476 AGGGCCC [GA]G^G[ACGT]CC[CT] 2126 GGGGCCT EcoRI 1069 GAATTC G^AATTC 1906 GAATTC FspI 356 TGCGCA TGC^GCA 1148 TGCGCA HgaI 982 GACGC GACGC(5/10) 636 GCGTC Hin1I 635 GGCGTC G[GA]^CG[CT]C 982 GACGCC Ksp632I 1009 CTCTTC CTCTTC(1/4) 1465 CTCTTC MfeI 432 CAATTG C^AATTG 1817 CAATTG MflI 863 AGATCT [GA]^GATC[CT] 1340 AGATCC MslI 799 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1251 CATGAAAGTG MunI 432 CAATTG C^AATTG 1817 CAATTG PstI 517 CTGCAG CTGCA^G 1327 CTGCAG PvuII 514 CAGCTG CAG^CTG 610 CAGCTG SacI 1462 GAGCTC GAGCT^C 1951 GAGCTC SspBI 34 TGTACA T^GTACA 1749 TGTACA XhoII 863 AGATCT [GA]^GATC[CT] 1340 AGATCC XmnI 543 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2160 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BalI BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI