################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BpmI BsaBI BseAI BseRI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MaeIII MamI MfeI MflI MroI MseI MunI NdeI NheI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AgeI 58 ACCGGT A^CCGGT AlwI 198 GATCC GGATC(4/5) AspHI 1054 GAGCTC G[AT]GC[AT]^C BalI 5 TGGCCA TGG^CCA BbsI 403 GAAGAC GAAGAC(2/6) BbvCI 315 CCTCAGC CCTCAGC(-5/-2) BlpI 963 GCTCAGC GC^T[ACGT]AGC Bpu1102I 963 GCTCAGC GC^T[ACGT]AGC BpuAI 403 GAAGAC GAAGAC(2/6) BsaAI 175 TACGTG [CT]AC^GT[GA] BsaI 55 GAGACC GGTCTC(1/5) BsaWI 58 ACCGGT [AT]^CCGG[AT] BsiHKAI 1054 GAGCTC G[AT]GC[AT]^C BsmAI 55 GAGAC GTCTC(1/5) BsmI 1110 GCATTC GAATGC(1/-1) BspLU11I 407 ACATGT A^CATGT BspMI 581 ACCTGC ACCTGC(4/8) BsrDI 1069 CATTGC GCAATG(2/0) BssHII 1103 GCGCGC G^CGCGC BssSI 606 CTCGTG CACGAG(-5/-1) CelII 963 GCTCAGC GC^T[ACGT]AGC EcoT14I 191 CCATGG C^C[AT][AT]GG EcoT22I 1066 ATGCAT ATGCA^T HphI 631 TCACC GGTGA(8/7) KspI 698 CCGCGG CCGC^GG MaeII 176 ACGT A^CGT MluI 777 ACGCGT A^CGCGT MluNI 5 TGGCCA TGG^CCA MscI 5 TGGCCA TGG^CCA NaeI 840 GCCGGC GCC^GGC NcoI 191 CCATGG C^CATGG NgoMIV 840 GCCGGC G^CCGGC NruI 254 TCGCGA TCG^CGA NsiI 1066 ATGCAT ATGCA^T NspI 407 ACATGT [GA]CATG^[CT] PinAI 58 ACCGGT A^CCGGT PvuII 683 CAGCTG CAG^CTG SacI 1054 GAGCTC GAGCT^C SacII 698 CCGCGG CCGC^GG SapI 495 GCTCTTC GCTCTTC(1/4) StyI 191 CCATGG C^C[AT][AT]GG TspRI 1014 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 407 ACATGT A^C[GA][CT]GT 777 ACGCGT AlwNI 671 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 680 CAGCAGCTG BsgI 895 CTGCAC GTGCAG(16/14) 781 GTGCAG BsiWI 173 CGTACG C^GTACG 761 CGTACG BsmFI 127 GGGAC GGGAC(10/14) 1082 GGGAC BstAPI 670 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 679 GCAGCAGCTGC BstF5I 303 CATCC GGATG(2/0) 877 CATCC DdeI 316 CTCAG C^T[ACGT]AG 964 CTCAG DpnII 198 GATC ^GATC 1123 GATC FokI 303 CATCC GGATG(9/13) 877 CATCC HgaI 28 GACGC GACGC(5/10) 384 GACGC MboI 198 GATC ^GATC 1123 GATC NdeII 198 GATC ^GATC 1123 GATC NotI 721 GCGGCCGC GC^GGCCGC 901 GCGGCCGC Sau3AI 198 GATC ^GATC 1123 GATC TaqI 413 TCGA T^CGA 1121 TCGA TthHB8I 413 TCGA T^CGA 1121 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI SfiI SfoI TseI