Restriction map for
pFN21AB9604
(Circular) (Six-base) MAPSORT of: pFN21AB9604.seq Check: 8116 from: 1 to: 5833
pFN21AB9604 5833 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2412 2782 4732 5709 1203
Size: 1209 370 1950 977 1327
Fragments arranged by size:
1950 1327 1209 977 370
AgeI A'CCGG_T
Cuts at: 2063 4664 2063
Size: 2601 3232
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 2783 1166
Size: 1617 4216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2545 2816 1184
Size: 1361 271 4201
Fragments arranged by size:
4201 1361 271
AlwNI CAG_nnn'CTG
Cuts at: 2681 2690 3576 5148 2681
Size: 9 886 1572 3366
Fragments arranged by size:
3366 1572 886 9
ApaI G_GGCC'C
Cuts at: 2047 2711 2756 2047
Size: 664 45 5124
Fragments arranged by size:
5124 664 45
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2027 3056 3158 1870
Size: 73 78 6 1029 102 4545
Fragments arranged by size:
4545 1029 102 78 73 6
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2347 2436 2516 2543 2572
Size: 325 217 627 560 89 80 27 29
Cuts at: 2572 3004 3154 4609 618
Size: 432 150 1455 1842
Fragments arranged by size:
1842 1455 627 560 432 325 217 150
89 80 29 27
BanII G_rGCy'C
Cuts at: 1391 2028 2047 2105 2502 2711 2756 3063 3152
Size: 637 19 58 397 209 45 307 89
Cuts at: 3152 1391
Size: 4072
Fragments arranged by size:
4072 637 397 307 209 89 58 45
19
BbeI G_GCGC'C
Cuts at: 2440 2520 2547 2576 3008 2440
Size: 80 27 29 432 5265
Fragments arranged by size:
5265 432 80 29 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 4346
Fragments arranged by size:
4346 971 516
BbvCI CC'TCA_GC
Cuts at: 2321 2321
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BfrBI ATG'CAT
Cuts at: 3073 3073
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2051 2089
Size: 107 122 71 631 605 337 42 38
Cuts at: 2089 2464 2678 2838 4444 136
Size: 375 214 160 1606 1525
Fragments arranged by size:
1606 1525 631 605 375 337 214 160
122 107 71 42 38
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 2969 3250 2969
Size: 281 5552
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2711 2756 3049 3894 5050 1790
Size: 257 664 45 293 845 1156 2573
Fragments arranged by size:
2573 1156 845 664 293 257 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3094 3564 4522
Size: 416 53 83 399 24 1677 470 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1677 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4493 1239
Size: 3254 2579
Bpu10I CC'TnA_GC
Cuts at: 2321 2321
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4496 882
Size: 617 555 2442 2219
Fragments arranged by size:
2442 2219 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3624 493
Size: 711 978 1442 2702
Fragments arranged by size:
2702 1442 978 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2437 2517 2544 2573
Size: 53 83 186 936 904 80 27 29
Cuts at: 2573 3005 3640 4022 4657 275
Size: 432 635 382 635 1451
Fragments arranged by size:
1451 936 904 635 635 432 382 186
83 80 53 29 27
BsaWI w'CCGG_w
Cuts at: 2063 4266 4664 4938 5085 5496 2063
Size: 2203 398 274 147 411 2400
Fragments arranged by size:
2400 2203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5833
BseYI C'CCAG_C
Cuts at: 2983 5036 5532 2983
Size: 2053 496 3284
Fragments arranged by size:
3284 2053 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 2885 1163
Size: 573 1071 78 4111
Fragments arranged by size:
4111 1071 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 2468 2730 2760 2850 2910
Size: 275 186 67 400 262 30 90 60
Cuts at: 2910 3283 4044 5072 1540
Size: 373 761 1028 2301
Fragments arranged by size:
2301 1028 761 400 373 275 262 186
90 67 60 30
BsiHKAI G_wGCw'C
Cuts at: 3063 3894 3979 5050 3063
Size: 831 85 1071 3846
Fragments arranged by size:
3846 1071 831 85
BsiWI C'GTAC_G
Cuts at: 2178 2766 2178
Size: 588 5245
BsmI GAATG_Cn'
Cuts at: 3115 3344 3437 3115
Size: 229 93 5511
Fragments arranged by size:
5511 229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2047 2105 2502 2711 2756 3049
Size: 399 238 19 58 397 209 45 293
Cuts at: 3049 3063 3152 3894 3979 5050 1391
Size: 14 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 293 238 209
89 85 58 45 19 14
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2595 3188 844
Size: 1751 593 3489
Fragments arranged by size:
3489 1751 593
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3073 4328 4502 65
Size: 1183 1825 1255 174 1396
Fragments arranged by size:
1825 1396 1255 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2433 2845 3104 4477 4664 1700
Size: 110 253 370 412 259 1373 187 2869
Fragments arranged by size:
2869 1373 412 370 259 253 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 3108 1925
Size: 1183 4650
BssSI C'ACGA_G
Cuts at: 2611 3893 4905 2611
Size: 1282 1012 3539
Fragments arranged by size:
3539 1282 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2681 2690 794
Size: 1887 9 3937
Fragments arranged by size:
3937 1887 9
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2196 2703 2938 513
Size: 552 1131 507 235 3408
Fragments arranged by size:
3408 1131 552 507 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 2242 487
Size: 683 56 950 66 4078
Fragments arranged by size:
4078 950 683 66 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2098 2133 529
Size: 35 1534 35 4229
Fragments arranged by size:
4229 1534 35 35
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2465 2615
Size: 54 1425 50 275 18 180 455 150
Cuts at: 2615 2727 2757 2847 2907 3280 4169 5798 8
Size: 112 30 90 60 373 889 1629 43
Fragments arranged by size:
1629 1425 889 455 373 275 180 150
112 90 60 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2465 2727 2757 2847 2907 3280 1537
Size: 275 653 262 30 90 60 373 4090
Fragments arranged by size:
4090 653 373 275 262 90 60 30
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2447 2507 3763 1353
Size: 863 231 60 1256 3423
Fragments arranged by size:
3423 1256 863 231 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2300 2853 4420 4794 4940 387
Size: 1064 138 711 553 1567 374 146 1280
Fragments arranged by size:
1567 1280 1064 711 553 374 146 138
EcoICRI GAG'CTC
Cuts at: 3061 3061
Size: 5833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3896 4493 5280 5548 1239
Size: 393 2264 597 787 268 1524
Fragments arranged by size:
2264 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2043 2044 2457 2708 2043
Size: 1 413 251 5168
Fragments arranged by size:
5168 413 251 1
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2518 2476
Size: 42 5791
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2189 2440 2520 2547 2576 2801 3008 4980
Size: 511 251 80 27 29 225 207 1972
Cuts at: 4980 1678
Size: 2531
Fragments arranged by size:
2531 1972 511 251 225 207 80 29
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373
Size: 32 135 32 2902 32 42 32 2626
Fragments arranged by size:
2902 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2436 2516 2543 2572 3004 2436
Size: 80 27 29 432 5265
Fragments arranged by size:
5265 432 80 29 27
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 2782 5709 2782
Size: 2927 2906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 10
Size: 54 1425 343 180 3831
Fragments arranged by size:
3831 1425 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2345 2783 3791 4150 4309 518
Size: 648 1029 150 438 1008 359 159 2042
Fragments arranged by size:
2042 1029 1008 648 438 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2678 2690 2705 2732 3249 3926 5074 5319
Size: 1072 12 15 27 517 677 1148 245
Cuts at: 5319 1606
Size: 2120
Fragments arranged by size:
2120 1148 1072 677 517 245 27 15
12
NaeI GCC'GGC
Cuts at: 2847 2847
Size: 5833
NarI GG'CG_CC
Cuts at: 2437 2517 2544 2573 3005 2437
Size: 80 27 29 432 5265
Fragments arranged by size:
5265 432 80 29 27
NcoI C'CATG_G
Cuts at: 513 1065 2196 513
Size: 552 1131 4150
Fragments arranged by size:
4150 1131 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 2845 2845
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 2727 2907 3280 2727
Size: 180 373 5280
Fragments arranged by size:
5280 373 180
NruI TCG'CGA
Cuts at: 2261 2261
Size: 5833
NsiI A_TGCA'T
Cuts at: 3075 3075
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2416 3191 4736 1132
Size: 97 1187 775 1545 2229
Fragments arranged by size:
2229 1545 1187 775 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 2412 4732 2412
Size: 2320 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2043 2707 2752 2043
Size: 664 45 5124
Fragments arranged by size:
5124 664 45
PstI C_TGCA'G
Cuts at: 830 2592 2686 2701 3036 3185 830
Size: 1762 94 15 335 149 3478
Fragments arranged by size:
3478 1762 335 149 94 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2690 2690
Size: 5833
SacI G_AGCT'C
Cuts at: 3063 3063
Size: 5833
SacII CC_GC'GG
Cuts at: 2706 2706
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SapI GCTCTTCn'nnn_
Cuts at: 2507 2507
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2588 2682 2697 3032 3181 4316 4997
Size: 219 1543 94 15 335 149 1135 681
Cuts at: 4997 5188 826
Size: 191 1471
Fragments arranged by size:
1543 1471 1135 681 335 219 191 149
94 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2464 2838 2051
Size: 413 374 5046
Fragments arranged by size:
5046 413 374
SfoI GGC'GCC
Cuts at: 2438 2518 2545 2574 3006 2438
Size: 80 27 29 432 5265
Fragments arranged by size:
5265 432 80 29 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2196 3272 513
Size: 552 1131 1076 3074
Fragments arranged by size:
3074 1131 1076 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 3860 3877 4030 4215 4660 325
Size: 1255 1073 1207 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1207 1073 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 4079 5791
Size: 274 80 33 51 1443 273 1829 1712
Cuts at: 5791 96
Size: 138
Fragments arranged by size:
1829 1712 1443 274 273 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI
SexAI SrfI SwaI XcmI