(Linear) (Six-base) MAPSORT of: KIBB9604.seq Check: 8282 from: 1 to: 1128 KIBB9604.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AflIII A'CryG_T Cuts at: 0 407 777 1128 Size: 407 370 351 Fragments arranged by size: 407 370 351 AgeI A'CCGG_T Cuts at: 0 58 1128 Size: 58 1070 AleI CACnn'nnGTG Cuts at: 0 778 1128 Size: 778 350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 540 811 1128 Size: 540 271 317 Fragments arranged by size: 540 317 271 AlwNI CAG_nnn'CTG Cuts at: 0 676 685 1128 Size: 676 9 443 Fragments arranged by size: 676 443 9 ApaI G_GGCC'C Cuts at: 0 42 706 751 1128 Size: 42 664 45 377 Fragments arranged by size: 664 377 45 42 AvaI C'yCGr_G Cuts at: 0 16 22 1051 1128 Size: 16 6 1029 77 Fragments arranged by size: 1029 77 16 6 BanI G'GyrC_C Cuts at: 0 342 431 511 538 567 999 1128 Size: 342 89 80 27 29 432 129 Fragments arranged by size: 432 342 129 89 80 29 27 BanII G_rGCy'C Cuts at: 0 23 42 100 497 706 751 1058 1128 Size: 23 19 58 397 209 45 307 70 Fragments arranged by size: 397 307 209 70 58 45 23 19 BbeI G_GCGC'C Cuts at: 0 435 515 542 571 1003 1128 Size: 435 80 27 29 432 125 Fragments arranged by size: 435 432 125 80 29 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 410 1128 Size: 410 718 BbvCI CC'TCA_GC Cuts at: 0 316 1128 Size: 316 812 BfrBI ATG'CAT Cuts at: 0 1068 1128 Size: 1068 60 BglI GCCn_nnn'nGGC Cuts at: 0 46 84 459 673 833 1128 Size: 46 38 375 214 160 295 Fragments arranged by size: 375 295 214 160 46 38 BlpI GC'TnA_GC Cuts at: 0 964 1128 Size: 964 164 Bme1580I G_kGCm'C Cuts at: 0 42 706 751 1044 1128 Size: 42 664 45 293 84 Fragments arranged by size: 664 293 84 45 42 BmrI ACTGGGnnnn_n' Cuts at: 0 1089 1128 Size: 1089 39 Bpu10I CC'TnA_GC Cuts at: 0 316 1128 Size: 316 812 BsaI GGTCTCn'nnnn_ Cuts at: 0 49 1128 Size: 49 1079 BsaAI yAC'GTr Cuts at: 0 177 1128 Size: 177 951 BsaHI Gr'CG_yC Cuts at: 0 432 512 539 568 1000 1128 Size: 432 80 27 29 432 128 Fragments arranged by size: 432 432 128 80 29 27 BsaWI w'CCGG_w Cuts at: 0 58 1128 Size: 58 1070 BseYI C'CCAG_C Cuts at: 0 978 1128 Size: 978 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 802 880 1128 Size: 802 78 248 Fragments arranged by size: 802 248 78 BsiEI CG_ry'CG Cuts at: 0 63 463 725 755 845 905 1128 Size: 63 400 262 30 90 60 223 Fragments arranged by size: 400 262 223 90 63 60 30 BsiHKAI G_wGCw'C Cuts at: 0 1058 1128 Size: 1058 70 BsiWI C'GTAC_G Cuts at: 0 173 761 1128 Size: 173 588 367 Fragments arranged by size: 588 367 173 BsmI GAATG_Cn' Cuts at: 0 1110 1128 Size: 1110 18 Bsp1286I G_dGCh'C Cuts at: 0 23 42 100 497 706 751 1044 1058 Size: 23 19 58 397 209 45 293 14 Cuts at: 1058 1128 Size: 70 Fragments arranged by size: 397 293 209 70 58 45 23 19 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 590 1128 Size: 590 538 BsrDI GCAATG_nn' Cuts at: 0 1068 1128 Size: 1068 60 BsrFI r'CCGG_y Cuts at: 0 58 428 840 1099 1128 Size: 58 370 412 259 29 Fragments arranged by size: 412 370 259 58 29 BssHII G'CGCG_C Cuts at: 0 1103 1128 Size: 1103 25 BssSI C'ACGA_G Cuts at: 0 606 1128 Size: 606 522 BstAPI GCAn_nnn'nTGC Cuts at: 0 676 685 1128 Size: 676 9 443 Fragments arranged by size: 676 443 9 BtgI C'CryG_G Cuts at: 0 191 698 933 1128 Size: 191 507 235 195 Fragments arranged by size: 507 235 195 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 171 237 1128 Size: 171 66 891 Fragments arranged by size: 891 171 66 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 93 128 1128 Size: 93 35 1000 Fragments arranged by size: 1000 93 35 EaeI y'GGCC_r Cuts at: 0 5 460 610 722 752 842 902 1128 Size: 5 455 150 112 30 90 60 226 Fragments arranged by size: 455 226 150 112 90 60 30 5 EagI C'GGCC_G Cuts at: 0 460 722 752 842 902 1128 Size: 460 262 30 90 60 226 Fragments arranged by size: 460 262 226 90 60 30 EarI CTCTTCn'nnn_ Cuts at: 0 211 442 502 1128 Size: 211 231 60 626 Fragments arranged by size: 626 231 211 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 295 848 1128 Size: 295 553 280 Fragments arranged by size: 553 295 280 EcoICRI GAG'CTC Cuts at: 0 1056 1128 Size: 1056 72 EcoO109I rG'GnC_Cy Cuts at: 0 38 39 452 703 1128 Size: 38 1 413 251 425 Fragments arranged by size: 425 413 251 38 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 471 513 1128 Size: 471 42 615 Fragments arranged by size: 615 471 42 HaeII r_GCGC'y Cuts at: 0 184 435 515 542 571 796 1003 1128 Size: 184 251 80 27 29 225 207 125 Fragments arranged by size: 251 225 207 184 125 80 29 27 KasI G'GCGC_C Cuts at: 0 431 511 538 567 999 1128 Size: 431 80 27 29 432 129 Fragments arranged by size: 432 431 129 80 29 27 MluI A'CGCG_T Cuts at: 0 777 1128 Size: 777 351 MscI TGG'CCA Cuts at: 0 7 1128 Size: 7 1121 MslI CAynn'nnrTG Cuts at: 0 190 340 778 1128 Size: 190 150 438 350 Fragments arranged by size: 438 350 190 150 MspA1I CmG'CkG Cuts at: 0 673 685 700 727 1128 Size: 673 12 15 27 401 Fragments arranged by size: 673 401 27 15 12 NaeI GCC'GGC Cuts at: 0 842 1128 Size: 842 286 NarI GG'CG_CC Cuts at: 0 432 512 539 568 1000 1128 Size: 432 80 27 29 432 128 Fragments arranged by size: 432 432 128 80 29 27 NcoI C'CATG_G Cuts at: 0 191 1128 Size: 191 937 NgoMIV G'CCGG_C Cuts at: 0 840 1128 Size: 840 288 NotI GC'GGCC_GC Cuts at: 0 722 902 1128 Size: 722 180 226 Fragments arranged by size: 722 226 180 NruI TCG'CGA Cuts at: 0 256 1128 Size: 256 872 NsiI A_TGCA'T Cuts at: 0 1070 1128 Size: 1070 58 NspI r_CATG'y Cuts at: 0 411 1128 Size: 411 717 PciI A'CATG_T Cuts at: 0 407 1128 Size: 407 721 PspOMI G'GGCC_C Cuts at: 0 38 702 747 1128 Size: 38 664 45 381 Fragments arranged by size: 664 381 45 38 PstI C_TGCA'G Cuts at: 0 587 681 696 1031 1128 Size: 587 94 15 335 97 Fragments arranged by size: 587 335 97 94 15 PvuII CAG'CTG Cuts at: 0 685 1128 Size: 685 443 SacI G_AGCT'C Cuts at: 0 1058 1128 Size: 1058 70 SacII CC_GC'GG Cuts at: 0 701 1128 Size: 701 427 SapI GCTCTTCn'nnn_ Cuts at: 0 502 1128 Size: 502 626 SfcI C'TryA_G Cuts at: 0 583 677 692 1027 1128 Size: 583 94 15 335 101 Fragments arranged by size: 583 335 101 94 15 SfiI GGCCn_nnn'nGGCC Cuts at: 0 46 459 833 1128 Size: 46 413 374 295 Fragments arranged by size: 413 374 295 46 SfoI GGC'GCC Cuts at: 0 433 513 540 569 1001 1128 Size: 433 80 27 29 432 127 Fragments arranged by size: 433 432 127 80 29 27 StyI C'CwwG_G Cuts at: 0 191 1128 Size: 191 937 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 648 1128 Size: 648 480 TatI w'GTAC_w Cuts at: 0 245 1128 Size: 245 883 Enzymes that do cut: AflIII AgeI AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI BlpI Bme1580I BmrI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstAPI BtgI BtgZI CspCI EaeI EagI EarI EciI EcoICRI EcoO109I FalI HaeII KasI MluI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI PciI PspOMI PstI PvuII SacI SacII SapI SfcI SfiI SfoI StyI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BglII BmgBI BmtI BplI BpmI BpuEI BsaBI BsaXI BseRI BsmBI BspEI BspHI BsrBI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI Eco57MI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MmeI NdeI NheI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI