################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 479 ACATGT A^C[GA][CT]GT AocI 739 CCTCAGG CC^T[ACGT]AGG ApaI 564 GGGCCC GGGCC^C AspHI 614 GAGCTC G[AT]GC[AT]^C AspI 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 268 GAAGAC GAAGAC(2/6) BbvI 597 GCAGC GCAGC(8/12) BcnI 660 CCGGG CC^[GC]GG BfaI 784 CTAG C^TAG BpmI 446 CTGGAG CTGGAG(16/14) BpuAI 268 GAAGAC GAAGAC(2/6) BseRI 451 GAGGAG GAGGAG(10/8) BsiHKAI 614 GAGCTC G[AT]GC[AT]^C BsiYI 576 CCCCAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 576 CCCCAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 608 GTCTC GTCTC(1/5) BsmFI 662 GGGAC GGGAC(10/14) BspHI 353 TCATGA T^CATGA BspLU11I 479 ACATGT A^CATGT BsrDI 692 CATTGC GCAATG(2/0) Bsu36I 739 CCTCAGG CC^T[ACGT]AGG Cac8I 176 GCGAGC GC[ACGT]^[ACGT]GC Eco81I 739 CCTCAGG CC^T[ACGT]AGG Fnu4HI 597 GCAGC GC^[ACGT]GC HapII 660 CCGG C^CGG HpaII 660 CCGG C^CGG ItaI 597 GCAGC GC^[ACGT]GC MaeI 784 CTAG C^TAG MaeII 536 ACGT A^CGT MslI 627 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 660 CCGG C^CGG MwoI 44 GCTGGTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 660 CCGGG CC^[GC]GG NspI 479 ACATGT [GA]CATG^[CT] PflFI 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 748 GAGTC GAGTC(4/5) PpuMI 289 AGGACCT [GA]G^G[AT]CC[CT] RcaI 353 TCATGA T^CATGA SacI 614 GAGCTC GAGCT^C SapI 696 GCTCTTC GCTCTTC(1/4) TfiI 360 GATTC G^A[AT]TC TseI 597 GCAGC G^C[AT]GC Tth111I 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 435 CCAACACAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 380 AAATTC [GA]^AATT[CT] 712 AAATTT AlwI 507 GATCC GGATC(4/5) 92 GGATC ApoI 380 AAATTC [GA]^AATT[CT] 712 AAATTT BalI 302 TGGCCA TGG^CCA 584 TGGCCA BanII 564 GGGCCC G[GA]GC[CT]^C 614 GAGCTC BmyI 564 GGGCCC G[AGT]GC[ACT]^C 614 GAGCTC BsaBI 31 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 87 GATGAGGATC BsgI 115 CTGCAC GTGCAG(16/14) 483 GTGCAG Bsp1286I 564 GGGCCC G[AGT]GC[ACT]^C 614 GAGCTC DraII 289 AGGACCT [GA]G^G[ACGT]CC[CT] 564 GGGCCCC EaeI 302 TGGCCA [CT]^GGCC[GA] 584 TGGCCA EcoO109I 289 AGGACCT [GA]G^G[ACGT]CC[CT] 564 GGGCCCC HinfI 360 GATTC G^A[ACGT]TC 748 GAGTC MaeIII 541 GTCAC ^GT[ACGT]AC 572 GTGAC MamI 31 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 87 GATGAGGATC MluNI 302 TGGCCA TGG^CCA 584 TGGCCA MscI 302 TGGCCA TGG^CCA 584 TGGCCA MseI 203 TTAA T^TAA 311 TTAA SfaNI 319 GATGC GCATC(5/9) 673 GATGC Tru9I 203 TTAA T^TAA 311 TTAA Tsp45I 541 GTCAC ^GT[GC]AC 572 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI AvaII BsaJI BsrI BssKI BstNI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I HaeIII HphI Ksp632I MboI MboII MnlI MvaI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI Tsp509I TspRI TthHB8I