(Circular) (Six-base) MAPSORT of: pFN21AE3095.seq Check: 3296 from: 1 to: 5494 pFN21AE3095 5494 bp With 182 enzymes: SgfI * March 28, 2012 13:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3557 4941 5209 1632 Size: 1925 1384 268 1917 Fragments arranged by size: 1925 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 2484 4393 5370 1203 Size: 1281 1909 977 1327 Fragments arranged by size: 1909 1327 1281 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2654 1184 Size: 1470 4024 AlwNI CAG_nnn'CTG Cuts at: 3237 4809 3237 Size: 1572 3922 ApaI G_GGCC'C Cuts at: 2573 2573 Size: 5494 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2385 2717 2803 3029 3181 5290 1493 Size: 892 332 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 892 332 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2819 1870 Size: 73 876 4545 Fragments arranged by size: 4545 876 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2815 4270 618 Size: 325 217 627 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 627 325 217 BanII G_rGCy'C Cuts at: 1391 2573 2623 2813 1391 Size: 1182 50 190 4072 Fragments arranged by size: 4072 1182 190 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 928 Size: 971 381 4142 Fragments arranged by size: 4142 971 381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3685 3719 1903 Size: 34 50 34 1664 34 3678 Fragments arranged by size: 3678 1664 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2573 3555 4711 1790 Size: 783 982 1156 2573 Fragments arranged by size: 2573 1156 982 783 BmgBI CAC'GTC Cuts at: 1318 2542 1318 Size: 1224 4270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2703 3225 4183 Size: 416 53 83 399 24 1286 522 958 Cuts at: 4183 442 Size: 1753 Fragments arranged by size: 1753 1286 958 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2472 4154 1239 Size: 1233 1682 2579 Fragments arranged by size: 2579 1682 1233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3285 493 Size: 711 2081 2702 Fragments arranged by size: 2702 2081 711 BsaBI GATnn'nnATC Cuts at: 2040 2096 5488 2040 Size: 56 3392 2046 Fragments arranged by size: 3392 2046 56 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5269 Fragments arranged by size: 5269 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 1186 Size: 1285 4209 BseYI C'CCAG_C Cuts at: 2375 4697 5193 2375 Size: 2322 496 2676 Fragments arranged by size: 2676 2322 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 2509 1163 Size: 573 369 404 4148 Fragments arranged by size: 4148 573 404 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2623 3555 3640 4711 2623 Size: 932 85 1071 3406 Fragments arranged by size: 3406 1071 932 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 2623 2813 3555 3640 4711 1391 Size: 399 783 50 190 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 190 85 50 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 2358 3383 5113 2358 Size: 1025 1730 2739 Fragments arranged by size: 2739 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2849 844 Size: 2005 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2696 3989 4163 65 Size: 1183 1448 1293 174 1396 Fragments arranged by size: 1448 1396 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4138 4325 1700 Size: 110 2328 187 2869 Fragments arranged by size: 2869 2328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5494 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489 Size: 852 367 385 17 1452 86 12 346 Cuts at: 5489 1972 Size: 1977 Fragments arranged by size: 1977 1452 852 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2745 2745 Size: 5494 BtgI C'CryG_G Cuts at: 513 1065 2248 2335 2656 513 Size: 552 1183 87 321 3351 Fragments arranged by size: 3351 1183 552 321 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 2334 2497 3010 3804 3824 2334 Size: 163 513 794 20 4004 Fragments arranged by size: 4004 794 513 163 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 2589 2941 Size: 54 1425 50 275 18 477 282 352 Cuts at: 2941 3830 5459 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 477 352 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2394 2708 3424 1353 Size: 734 307 314 716 3423 Fragments arranged by size: 3423 734 716 314 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2621 2621 Size: 5494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2472 3557 4154 4941 5209 1239 Size: 393 840 1085 597 787 268 1524 Fragments arranged by size: 1524 1085 840 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2295 2570 2295 Size: 275 5219 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2447 2447 Size: 5494 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373 Size: 32 135 32 2563 32 42 32 2626 Fragments arranged by size: 2626 2563 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4608 4792 1305 Size: 3303 184 2007 Fragments arranged by size: 3303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 2591 10 Size: 54 1425 343 477 282 2913 Fragments arranged by size: 2913 1425 477 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 3452 3811 3970 518 Size: 648 1470 816 359 159 2042 Fragments arranged by size: 2042 1470 816 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2910 3587 4735 4980 1606 Size: 1304 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2248 2335 2656 513 Size: 552 1183 87 321 3351 Fragments arranged by size: 3351 1183 552 321 87 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2488 2852 4397 1132 Size: 97 1259 364 1545 2229 Fragments arranged by size: 2229 1545 1259 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 2484 4393 2484 Size: 1909 3585 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2295 2295 Size: 5494 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PspOMI G'GGCC_C Cuts at: 2569 2569 Size: 5494 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 SacI G_AGCT'C Cuts at: 2623 2623 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SapI GCTCTTCn'nnn_ Cuts at: 2708 2708 Size: 5494 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2842 3977 4658 4849 826 Size: 219 1797 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3598 4499 4761 820 Size: 197 926 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2248 2304 2335 2656 2768 2933 513 Size: 552 1183 56 31 321 112 165 3074 Fragments arranged by size: 3074 1183 552 321 165 112 56 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2759 3740 Size: 274 80 33 51 1443 189 593 981 Cuts at: 3740 5452 96 Size: 1712 138 Fragments arranged by size: 1712 1443 981 593 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 2543 1316 Size: 1227 4267 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2447 2447 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI