(Circular) (Six-base) MAPSORT of: pFN21AE6684.seq Check: 3107 from: 1 to: 5725 pFN21AE6684 5725 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 2343 3852 4225 1563 Size: 780 1509 373 3063 Fragments arranged by size: 3063 1509 780 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 3788 5172 5440 1632 Size: 467 1689 1384 268 1917 Fragments arranged by size: 1917 1689 1384 467 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2151 2924 1166 Size: 985 773 3967 Fragments arranged by size: 3967 985 773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 2128 2999 3468 5040 2128 Size: 871 469 1572 2813 Fragments arranged by size: 2813 1572 871 469 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 3050 1870 Size: 73 1107 4545 Fragments arranged by size: 4545 1107 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2560 2593 2560 Size: 33 5692 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 3044 1391 Size: 628 1025 4072 Fragments arranged by size: 4072 1025 628 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2388 2727 928 Size: 971 489 339 3926 Fragments arranged by size: 3926 971 489 339 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2546 2580 3916 3950 1903 Size: 34 50 34 525 34 1336 34 3678 Fragments arranged by size: 3678 1336 525 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2824 3617 4827 2824 Size: 793 1210 3722 Fragments arranged by size: 3722 1210 793 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BfrBI ATG'CAT Cuts at: 2459 2625 2459 Size: 166 5559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 3786 4942 1790 Size: 1996 1156 2573 Fragments arranged by size: 2573 1996 1156 BmgBI CAC'GTC Cuts at: 1318 2153 1318 Size: 835 4890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3009 3456 4414 Size: 416 53 83 399 24 1592 447 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1592 958 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2003 4385 1239 Size: 764 2382 2579 Fragments arranged by size: 2579 2382 764 Bpu10I CC'TnA_GC Cuts at: 2141 2141 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 2209 4388 882 Size: 617 666 44 2179 2219 Fragments arranged by size: 2219 2179 666 617 44 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 1178 Size: 30 165 30 1374 30 4096 Fragments arranged by size: 4096 1374 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 1186 Size: 1366 4359 BseYI C'CCAG_C Cuts at: 2311 3007 4928 5424 2311 Size: 696 1921 496 2612 Fragments arranged by size: 2612 1921 696 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 3786 3871 4942 2019 Size: 1767 85 1071 2802 Fragments arranged by size: 2802 1767 1071 85 BsmI GAATG_Cn' Cuts at: 2627 3236 3329 2627 Size: 609 93 5023 Fragments arranged by size: 5023 609 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 3044 3786 3871 4942 1391 Size: 399 229 1025 742 85 1071 2174 Fragments arranged by size: 2174 1071 1025 742 399 229 85 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3080 844 Size: 2236 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2153 Size: 5725 BstYI r'GATC_y Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720 Size: 1083 367 385 17 1452 86 12 346 Cuts at: 5720 1972 Size: 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2535 2744 2535 Size: 209 5516 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 3241 4035 4055 3241 Size: 794 20 4911 Fragments arranged by size: 4911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2181 2216 529 Size: 35 1617 35 4038 Fragments arranged by size: 4038 1617 35 35 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2510 4732 809 Size: 507 1194 2222 1802 Fragments arranged by size: 2222 1802 1194 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 3172 4061 Size: 54 1425 50 275 18 639 703 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 703 639 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2357 3655 1353 Size: 746 258 1298 3423 Fragments arranged by size: 3423 1298 746 258 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2017 2017 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2003 2099 3788 4385 5172 5440 1239 Size: 393 371 96 1689 597 787 268 1524 Fragments arranged by size: 1689 1524 787 597 393 371 268 96 EcoO109I rG'GnC_Cy Cuts at: 2675 2968 2675 Size: 293 5432 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 2556 4231 2556 Size: 1675 4050 HaeII r_GCGC'y Cuts at: 1678 2815 4872 1678 Size: 1137 2057 2531 Fragments arranged by size: 2531 2057 1137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 4366 4398 4440 Size: 32 135 32 929 32 1833 32 42 Cuts at: 4440 4472 1373 Size: 32 2626 Fragments arranged by size: 2626 1833 929 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 2842 748 Size: 2094 3631 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4839 5023 1305 Size: 3534 184 2007 Fragments arranged by size: 3534 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 10 Size: 54 1425 343 639 3264 Fragments arranged by size: 3264 1425 639 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2288 2498 2924 3683 4042 4201 Size: 648 985 137 210 426 759 359 159 Cuts at: 4201 518 Size: 2042 Fragments arranged by size: 2042 985 759 648 426 359 210 159 137 MspA1I CmG'CkG Cuts at: 1606 2088 3141 3818 4966 5211 1606 Size: 482 1053 677 1148 245 2120 Fragments arranged by size: 2120 1148 1053 677 482 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NsiI A_TGCA'T Cuts at: 2461 2627 2461 Size: 166 5559 NspI r_CATG'y Cuts at: 1132 1229 3083 4628 1132 Size: 97 1854 1545 2229 Fragments arranged by size: 2229 1854 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 2478 1108 Size: 1020 350 4355 Fragments arranged by size: 4355 1020 350 PfoI T'CCnGG_A Cuts at: 2727 2727 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2968 2968 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2088 2088 Size: 5725 SacI G_AGCT'C Cuts at: 2019 2019 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 3073 4208 4889 5080 826 Size: 219 2028 1135 681 191 1471 Fragments arranged by size: 2028 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 4730 4992 820 Size: 197 926 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 2586 3649 5 Size: 47 2534 1063 2081 Fragments arranged by size: 2534 2081 1063 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 2671 3164 513 Size: 552 1606 493 3074 Fragments arranged by size: 3074 1606 552 493 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2473 2505 5685 5717 2473 Size: 32 3180 32 2481 Fragments arranged by size: 3180 2481 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 2167 3061 2167 Size: 894 4831 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2397 2397 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI