################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 47 GTCGAC GT^[AC][GT]AC AciI 511 CCGC CCGC(-3/-1) AcsI 487 GAATTT [GA]^AATT[CT] AcyI 31 GGCGCC G[GA]^CG[CT]C AflIII 1007 ACGTGT A^C[GA][CT]GT AlwI 924 GGATC GGATC(4/5) ApaI 78 GGGCCC GGGCC^C ApoI 487 GAATTT [GA]^AATT[CT] AvaII 329 GGACC G^G[AT]CC BanI 31 GGCGCC G^G[CT][GA]CC BanII 78 GGGCCC G[GA]GC[CT]^C BbeI 31 GGCGCC GGCGC^C BbsI 119 GTCTTC GAAGAC(2/6) BmyI 78 GGGCCC G[AGT]GC[ACT]^C BpmI 719 CTGGAG CTGGAG(16/14) BpuAI 119 GTCTTC GAAGAC(2/6) BsaBI 148 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 31 GGCGCC G[GA]^CG[CT]C BseRI 572 CTCCTC GAGGAG(10/8) Bsp1286I 78 GGGCCC G[AGT]GC[ACT]^C BspHI 653 TCATGA T^CATGA BspMI 24 GCAGGT ACCTGC(4/8) BsrI 733 ACTGG ACTGG(1/-1) BstYI 924 GGATCT [GA]^GATC[CT] DdeI 885 CTTAG C^T[ACGT]AG DpnII 925 GATC ^GATC DraI 670 TTTAAA TTT^AAA DrdI 376 GACTGTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 250 CCATGG C^C[GA][CT]GG EarI 973 GAAGAG CTCTTC(1/4) HaeII 31 GGCGCC [GA]GCGC^[CT] HgaI 21 GACGC GACGC(5/10) Hin1I 31 GGCGCC G[GA]^CG[CT]C HincII 47 GTCGAC GT[CT]^[GA]AC HindII 47 GTCGAC GT[CT]^[GA]AC HphI 736 GGTGA GGTGA(8/7) KasI 31 GGCGCC G^GCGCC Ksp632I 973 GAAGAG CTCTTC(1/4) MamI 148 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 925 GATC ^GATC MflI 924 GGATCT [GA]^GATC[CT] NarI 31 GGCGCC GG^CGCC NcoI 250 CCATGG C^CATGG NdeII 925 GATC ^GATC NspI 348 GCATGT [GA]CATG^[CT] PflMI 36 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 21 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 653 TCATGA T^CATGA SalI 47 GTCGAC G^TCGAC Sau3AI 925 GATC ^GATC SexAI 273 ACCAGGT A^CC[AT]GGT SfcI 437 CTGTAG C^T[GA][CT]AG SfoI 31 GGCGCC GGC^GCC SmaI 81 CCCGGG CCC^GGG TaqI 48 TCGA T^CGA TfiI 901 GATTC G^A[AT]TC TthHB8I 48 TCGA T^CGA Van91I 36 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 708 CCACAGGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 924 GGATCT [GA]^GATC[CT] XmaCI 81 CCCGGG C^CCGGG XmaI 81 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 37 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 747 CAGAGTCTG AvaI 52 CTCGGG C^[CT]CG[GA]G 81 CCCGGG BsaI 714 GAGACC GGTCTC(1/5) 192 GGTCTC BsiYI 36 CCAGGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCTGGGCCCGG BslI 36 CCAGGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCTGGGCCCGG BsmAI 714 GAGAC GTCTC(1/5) 193 GTCTC BsmFI 56 GGGAC GGGAC(10/14) 172 GTCCC BsoBI 52 CTCGGG C^[CT]CG[GA]G 81 CCCGGG CfoI 32 GCGC GCG^C 1024 GCGC HhaI 32 GCGC GCG^C 1024 GCGC HinP1I 32 GCGC G^CGC 1024 GCGC MaeIII 355 GTCAC ^GT[ACGT]AC 616 GTCAC PleI 50 GACTC GAGTC(4/5) 749 GAGTC SfaNI 318 GATGC GCATC(5/9) 153 GCATC Tsp45I 355 GTCAC ^GT[GC]AC 616 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI BbvI BcnI BsaJI BssKI BstF5I BstNI Cac8I Cfr13I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MboII MnlI MseI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI StyI Tru9I TseI Tsp509I