################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 610 GTCTAC GT^[AC][GT]AC AflIII 686 ACGTGT A^C[GA][CT]GT BbsI 106 GAAGAC GAAGAC(2/6) BcgI 1004 GCACGCAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 298 GTATCC GTATCC(6/5) BclI 395 TGATCA T^GATCA BpuAI 106 GAAGAC GAAGAC(2/6) BsaWI 128 TCCGGT [AT]^CCGG[AT] BsmAI 898 GAGAC GTCTC(1/5) Bsp1407I 915 TGTACA T^GTACA BsrBI 817 CCGCTC CCGCTC(-3/-3) BsrDI 282 CATTGC GCAATG(2/0) BsrFI 1021 GCCGGC [GA]^CCGG[CT] BsrGI 915 TGTACA T^GTACA Cfr10I 1021 GCCGGC [GA]^CCGG[CT] EcoT14I 561 CCATGG C^C[AT][AT]GG FbaI 395 TGATCA T^GATCA HindIII 190 AAGCTT A^AGCTT HinfI 1015 GACTC G^A[ACGT]TC MseI 548 TTAA T^TAA NaeI 1021 GCCGGC GCC^GGC NcoI 561 CCATGG C^CATGG NgoMIV 1021 GCCGGC G^CCGGC PaeR7I 999 CTCGAG C^TCGAG PleI 1015 GACTC GAGTC(4/5) SapI 165 GCTCTTC GCTCTTC(1/4) SfiI 559 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 287 CCCGGG CCC^GGG SmlI 999 CTCGAG C^T[CT][GA]AG SspBI 915 TGTACA T^GTACA StyI 561 CCATGG C^C[AT][AT]GG Tru9I 548 TTAA T^TAA XhoI 999 CTCGAG C^TCGAG XmaCI 287 CCCGGG C^CCGGG XmaI 287 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Aor51HI 42 AGCGCT AGC^GCT 1097 AGCGCT BanI 60 GGCACC G^G[CT][GA]CC 115 GGCACC BanII 766 GGGCTC G[GA]GC[CT]^C 859 GAGCCC BbvCI 983 CCTCAGC CCTCAGC(-5/-2) 538 GCTGAGG BglI 560 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1064 GCCACACTGGC BsaAI 685 TACGTG [CT]AC^GT[GA] 826 TACGTG BsaBI 213 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 523 GATGCCCATC BstAPI 328 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 967 GCAGGGGCTGC DsaI 293 CCGTGG C^C[GA][CT]GG 561 CCATGG EarI 166 CTCTTC CTCTTC(1/4) 871 CTCTTC Eco47III 42 AGCGCT AGC^GCT 1097 AGCGCT EcoNI 9 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 717 CCTACTGCAGG Ksp632I 166 CTCTTC CTCTTC(1/4) 871 CTCTTC MaeII 686 ACGT A^CGT 827 ACGT MaeIII 19 GTGAC ^GT[ACGT]AC 490 GTCAC MamI 213 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 523 GATGCCCATC PpuMI 137 GGGACCT [GA]G^G[AT]CC[CT] 499 GGGACCC PstI 721 CTGCAG CTGCA^G 965 CTGCAG PvuII 536 CAGCTG CAG^CTG 986 CAGCTG SfcI 721 CTGCAG C^T[GA][CT]AG 965 CTGCAG Tsp45I 19 GTGAC ^GT[GC]AC 490 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I XhoII