(Circular) (Six-base) MAPSORT of: pFN21AE9464.seq Check: 3162 from: 1 to: 5824 pFN21AE9464 5824 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2616 3167 1937 Size: 679 551 4594 Fragments arranged by size: 4594 679 551 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 2791 3887 5271 5539 1632 Size: 1040 119 1096 1384 268 1917 Fragments arranged by size: 1917 1384 1096 1040 268 119 AfeI AGC'GCT Cuts at: 2049 3104 2049 Size: 1055 4769 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2691 4723 5700 1203 Size: 1488 2032 977 1327 Fragments arranged by size: 2032 1488 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2471 1166 Size: 1305 4519 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2684 1184 Size: 1500 4324 AlwNI CAG_nnn'CTG Cuts at: 2598 2734 2978 3567 5139 2598 Size: 136 244 589 1572 3283 Fragments arranged by size: 3283 1572 589 244 136 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2208 2292 3004 3149 1870 Size: 73 265 84 712 145 4545 Fragments arranged by size: 4545 712 265 145 84 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2120 3145 4600 618 Size: 325 217 627 278 55 1025 1455 1842 Fragments arranged by size: 1842 1455 1025 627 325 278 217 55 BanII G_rGCy'C Cuts at: 1391 2775 2868 3143 1391 Size: 1384 93 275 4072 Fragments arranged by size: 4072 1384 275 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 928 Size: 971 219 4634 Fragments arranged by size: 4634 971 219 BbvCI CC'TCA_GC Cuts at: 2544 2989 2544 Size: 445 5379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2998 3032 4015 4049 1903 Size: 34 1061 34 983 34 3678 Fragments arranged by size: 3678 1061 983 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2314 3716 4926 2314 Size: 1402 1210 3212 Fragments arranged by size: 3212 1402 1210 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 4972 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2571 3075 4435 Size: 107 122 71 631 605 899 504 1360 Cuts at: 4435 136 Size: 1525 Fragments arranged by size: 1525 1360 899 631 605 504 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 3885 5041 1790 Size: 2095 1156 2573 Fragments arranged by size: 2573 2095 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3555 4513 Size: 416 53 83 399 24 732 1406 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1406 958 732 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2110 2215 2450 2531 2984 4484 1239 Size: 797 74 105 235 81 453 1500 2579 Fragments arranged by size: 2579 1500 797 453 235 105 81 74 Bpu10I CC'TnA_GC Cuts at: 2544 2989 2544 Size: 445 5379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2692 2833 3615 493 Size: 711 1488 141 782 2702 Fragments arranged by size: 2702 1488 782 711 141 BsaBI GATnn'nnATC Cuts at: 2222 2532 5818 2222 Size: 310 3286 2228 Fragments arranged by size: 3286 2228 310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 4257 4655 4929 5076 5487 2133 Size: 2124 398 274 147 411 2470 Fragments arranged by size: 2470 2124 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 2504 2534 1178 Size: 30 165 30 1020 30 51 30 4468 Fragments arranged by size: 4468 1020 165 51 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2695 2924 1186 Size: 847 662 229 4086 Fragments arranged by size: 4086 847 662 229 BseYI C'CCAG_C Cuts at: 2734 2931 2981 5027 5523 2734 Size: 197 50 2046 496 3035 Fragments arranged by size: 3035 2046 496 197 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 2172 2321 3011 3885 3970 5041 2045 Size: 127 149 690 874 85 1071 2828 Fragments arranged by size: 2828 1071 874 690 149 127 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2172 2321 2775 2868 3011 3143 Size: 399 255 127 149 454 93 143 132 Cuts at: 3143 3885 3970 5041 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 255 149 143 132 127 93 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2824 3711 1394 Size: 429 1001 887 3507 Fragments arranged by size: 3507 1001 887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2286 4319 4493 65 Size: 1183 1038 2033 174 1396 Fragments arranged by size: 2033 1396 1183 1038 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3026 4468 4655 1700 Size: 110 1216 1442 187 2869 Fragments arranged by size: 2869 1442 1216 187 110 BsrGI T'GTAC_A Cuts at: 96 2920 96 Size: 2824 3000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2339 2978 794 Size: 1545 639 3640 Fragments arranged by size: 3640 1545 639 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2217 2349 2514 2871 3154 3521 3906 3923 Size: 245 132 165 357 283 367 385 17 Cuts at: 3923 5375 5461 5473 5819 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 357 346 283 245 165 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 2566 513 Size: 552 939 294 268 3771 Fragments arranged by size: 3771 939 552 294 268 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2072 3037 487 Size: 683 56 846 965 3274 Fragments arranged by size: 3274 965 846 683 56 BtsI GCAGTG_nn' Cuts at: 2711 3340 4134 4154 2711 Size: 629 794 20 4381 Fragments arranged by size: 4381 794 629 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2422 2460 Size: 54 1425 50 275 18 552 40 38 Cuts at: 2460 2563 3271 4160 5789 8 Size: 103 708 889 1629 43 Fragments arranged by size: 1629 1425 889 708 552 275 103 54 50 43 40 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2882 3754 1353 Size: 824 705 872 3423 Fragments arranged by size: 3423 872 824 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2110 2215 2450 2531 2672 2791 Size: 393 404 74 105 235 81 141 119 Cuts at: 2791 2984 3887 4484 5271 5539 1239 Size: 193 903 597 787 268 1524 Fragments arranged by size: 1524 903 787 597 404 393 268 235 193 141 119 105 81 74 EcoNI CCTnn'n_nnAGG Cuts at: 2018 2726 2018 Size: 708 5116 EcoO109I rG'GnC_Cy Cuts at: 2143 2505 2959 2985 2143 Size: 362 454 26 4982 Fragments arranged by size: 4982 454 362 26 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 5824 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2051 2279 3106 4971 1678 Size: 373 228 827 1865 2531 Fragments arranged by size: 2531 1865 827 373 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 4465 4497 4539 Size: 32 135 32 851 32 2010 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 2010 851 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 2195 748 Size: 1447 4377 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 2424 2462 2565 10 Size: 54 1425 343 552 40 38 103 3269 Fragments arranged by size: 3269 1425 552 343 103 54 40 38 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2261 2312 2471 2831 3782 4141 Size: 648 912 183 51 159 360 951 359 Cuts at: 4141 4300 518 Size: 159 2042 Fragments arranged by size: 2042 951 912 648 360 359 183 159 159 51 MspA1I CmG'CkG Cuts at: 1606 2543 2650 2993 3240 3917 5065 5310 1606 Size: 937 107 343 247 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 677 343 247 245 107 NaeI GCC'GGC Cuts at: 3028 3028 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2004 2566 513 Size: 552 939 562 3771 Fragments arranged by size: 3771 939 562 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 3026 3026 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 2500 1348 Size: 1152 4672 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2505 2143 Size: 362 5462 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspXI vC'TCGA_Gb Cuts at: 3004 3004 Size: 5824 PstI C_TGCA'G Cuts at: 830 2730 2974 3176 830 Size: 1900 244 202 3478 Fragments arranged by size: 3478 1900 244 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2543 2993 2543 Size: 450 5374 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SanDI GG'GwC_CC Cuts at: 2505 2505 Size: 5824 SapI GCTCTTCn'nnn_ Cuts at: 2177 2177 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2726 2970 3172 4307 4988 5179 826 Size: 219 1681 244 202 1135 681 191 1471 Fragments arranged by size: 1681 1471 1135 681 244 219 202 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2571 2571 Size: 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2294 3151 1872 Size: 422 857 4545 Fragments arranged by size: 4545 857 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 3004 3928 4829 5091 820 Size: 197 926 1061 924 901 262 1553 Fragments arranged by size: 1553 1061 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SrfI GCCC'GGGC Cuts at: 2294 2294 Size: 5824 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2004 2566 3263 513 Size: 552 939 562 697 3074 Fragments arranged by size: 3074 939 697 562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2513 3851 3868 4021 4206 4651 325 Size: 1255 933 1338 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 933 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 2920 4070 Size: 274 80 33 51 1443 825 118 1150 Cuts at: 4070 5782 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 825 274 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 3004 1943 Size: 1061 4763 XmaI C'CCGG_G Cuts at: 1870 2292 3149 1870 Size: 422 857 4545 Fragments arranged by size: 4545 857 422 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SexAI SfoI SwaI