Restriction map for
pFN21AE9464
(Linear) (Six-base) MAPSORT of: KIEE9464.seq Check: 1656 from: 1 to: 1119
KIEE9464.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 611 1119
Size: 611 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 786 1119
Size: 667 119 333
Fragments arranged by size:
667 333 119
AfeI AGC'GCT
Cuts at: 0 44 1099 1119
Size: 44 1055 20
Fragments arranged by size:
1055 44 20
AflIII A'CryG_T
Cuts at: 0 686 1119
Size: 686 433
AleI CACnn'nnGTG
Cuts at: 0 466 1119
Size: 466 653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 1119
Size: 679 440
AlwNI CAG_nnn'CTG
Cuts at: 0 593 729 973 1119
Size: 593 136 244 146
Fragments arranged by size:
593 244 146 136
AvaI C'yCGr_G
Cuts at: 0 203 287 999 1119
Size: 203 84 712 120
Fragments arranged by size:
712 203 120 84
BanI G'GyrC_C
Cuts at: 0 60 115 1119
Size: 60 55 1004
Fragments arranged by size:
1004 60 55
BanII G_rGCy'C
Cuts at: 0 770 863 1119
Size: 770 93 256
Fragments arranged by size:
770 256 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 1119
Size: 113 1006
BbvCI CC'TCA_GC
Cuts at: 0 539 984 1119
Size: 539 445 135
Fragments arranged by size:
539 445 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 993 1027 1119
Size: 993 34 92
Fragments arranged by size:
993 92 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 309 1119
Size: 309 810
BclI T'GATC_A
Cuts at: 0 395 1119
Size: 395 724
BglI GCCn_nnn'nGGC
Cuts at: 0 566 1070 1119
Size: 566 504 49
Fragments arranged by size:
566 504 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1119
Size: 144 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 675 1119
Size: 675 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 105 210 445 526 979 1119
Size: 31 74 105 235 81 453 140
Fragments arranged by size:
453 235 140 105 81 74 31
Bpu10I CC'TnA_GC
Cuts at: 0 539 984 1119
Size: 539 445 135
Fragments arranged by size:
539 445 135
BsaAI yAC'GTr
Cuts at: 0 687 828 1119
Size: 687 141 291
Fragments arranged by size:
687 291 141
BsaBI GATnn'nnATC
Cuts at: 0 217 527 1119
Size: 217 310 592
Fragments arranged by size:
592 310 217
BsaWI w'CCGG_w
Cuts at: 0 128 1119
Size: 128 991
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 499 529 1119
Size: 418 30 51 30 590
Fragments arranged by size:
590 418 51 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 690 919 1119
Size: 28 662 229 200
Fragments arranged by size:
662 229 200 28
BseYI C'CCAG_C
Cuts at: 0 729 926 976 1119
Size: 729 197 50 143
Fragments arranged by size:
729 197 143 50
BsiHKAI G_wGCw'C
Cuts at: 0 40 167 316 1006 1119
Size: 40 127 149 690 113
Fragments arranged by size:
690 149 127 113 40
Bsp1286I G_dGCh'C
Cuts at: 0 40 167 316 770 863 1006 1119
Size: 40 127 149 454 93 143 113
Fragments arranged by size:
454 149 143 127 113 93 40
BsrBI CCG'CTC
Cuts at: 0 819 1119
Size: 819 300
BsrDI GCAATG_nn'
Cuts at: 0 281 1119
Size: 281 838
BsrFI r'CCGG_y
Cuts at: 0 1021 1119
Size: 1021 98
BsrGI T'GTAC_A
Cuts at: 0 915 1119
Size: 915 204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 334 973 1119
Size: 334 639 146
Fragments arranged by size:
639 334 146
BstYI r'GATC_y
Cuts at: 0 212 344 509 866 1119
Size: 212 132 165 357 253
Fragments arranged by size:
357 253 212 165 132
BtgI C'CryG_G
Cuts at: 0 293 561 1119
Size: 293 268 558
Fragments arranged by size:
558 293 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 67 1032 1119
Size: 67 965 87
Fragments arranged by size:
965 87 67
BtsI GCAGTG_nn'
Cuts at: 0 706 1119
Size: 706 413
EaeI y'GGCC_r
Cuts at: 0 377 417 455 558 1119
Size: 377 40 38 103 561
Fragments arranged by size:
561 377 103 40 38
EarI CTCTTCn'nnn_
Cuts at: 0 172 877 1119
Size: 172 705 242
Fragments arranged by size:
705 242 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 105 210 445 526 667 786 979
Size: 31 74 105 235 81 141 119 193
Cuts at: 979 1119
Size: 140
Fragments arranged by size:
235 193 141 140 119 105 81 74
31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 13 721 1119
Size: 13 708 398
Fragments arranged by size:
708 398 13
EcoO109I rG'GnC_Cy
Cuts at: 0 138 500 954 980 1119
Size: 138 362 454 26 139
Fragments arranged by size:
454 362 139 138 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 888 1119
Size: 888 231
HaeII r_GCGC'y
Cuts at: 0 46 274 1101 1119
Size: 46 228 827 18
Fragments arranged by size:
827 228 46 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 1119
Size: 418 32 669
Fragments arranged by size:
669 418 32
HindIII A'AGCT_T
Cuts at: 0 190 1119
Size: 190 929
MscI TGG'CCA
Cuts at: 0 379 419 457 560 1119
Size: 379 40 38 103 559
Fragments arranged by size:
559 379 103 40 38
MslI CAynn'nnrTG
Cuts at: 0 73 256 307 466 826 1119
Size: 73 183 51 159 360 293
Fragments arranged by size:
360 293 183 159 73 51
MspA1I CmG'CkG
Cuts at: 0 538 645 988 1119
Size: 538 107 343 131
Fragments arranged by size:
538 343 131 107
NaeI GCC'GGC
Cuts at: 0 1023 1119
Size: 1023 96
NcoI C'CATG_G
Cuts at: 0 561 1119
Size: 561 558
NgoMIV G'CCGG_C
Cuts at: 0 1021 1119
Size: 1021 98
PasI CC'CwG_GG
Cuts at: 0 495 1119
Size: 495 624
PpuMI rG'GwC_Cy
Cuts at: 0 138 500 1119
Size: 138 362 619
Fragments arranged by size:
619 362 138
PspXI vC'TCGA_Gb
Cuts at: 0 999 1119
Size: 999 120
PstI C_TGCA'G
Cuts at: 0 725 969 1119
Size: 725 244 150
Fragments arranged by size:
725 244 150
PvuII CAG'CTG
Cuts at: 0 538 988 1119
Size: 538 450 131
Fragments arranged by size:
538 450 131
SanDI GG'GwC_CC
Cuts at: 0 500 1119
Size: 500 619
SapI GCTCTTCn'nnn_
Cuts at: 0 172 1119
Size: 172 947
SfcI C'TryA_G
Cuts at: 0 721 965 1119
Size: 721 244 154
Fragments arranged by size:
721 244 154
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 566 1119
Size: 566 553
SmaI CCC'GGG
Cuts at: 0 289 1119
Size: 289 830
SmlI C'TyrA_G
Cuts at: 0 999 1119
Size: 999 120
SrfI GCCC'GGGC
Cuts at: 0 289 1119
Size: 289 830
StyI C'CwwG_G
Cuts at: 0 561 1119
Size: 561 558
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 1119
Size: 508 611
TatI w'GTAC_w
Cuts at: 0 797 915 1119
Size: 797 118 204
Fragments arranged by size:
797 204 118
XhoI C'TCGA_G
Cuts at: 0 999 1119
Size: 999 120
XmaI C'CCGG_G
Cuts at: 0 287 1119
Size: 287 832
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BsrGI
BstAPI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PpuMI PspXI PstI
PvuII SanDI SapI SfcI SfiI SmaI SmlI SrfI
StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BfrBI BglII BlpI Bme1580I BmgBI BmtI
BpuEI BsaI BsaHI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
SwaI TstI Tth111I XbaI XcmI XmnI ZraI