Restriction map for
pFN21AE4428
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BpuAI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraII EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeI
MaeII MluI MroI MspA1I MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 840 GAATTT [GA]^AATT[CT]
AhdI 370 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 94 CCTCAGG CC^T[ACGT]AGG
ApoI 840 GAATTT [GA]^AATT[CT]
AseI 736 ATTAAT AT^TAAT
AsnI 736 ATTAAT AT^TAAT
Asp700I 837 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 370 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 159 GAGCAC G[AT]GC[AT]^C
BamHI 51 GGATCC G^GATCC
BglII 533 AGATCT A^GATCT
BlpI 121 GCTGAGC GC^T[ACGT]AGC
BmyI 159 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC
BsaBI 265 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 583 GAGACC GGTCTC(1/5)
BseRI 580 GAGGAG GAGGAG(10/8)
BsiHKAI 159 GAGCAC G[AT]GC[AT]^C
BsmI 554 GAATGC GAATGC(1/-1)
Bsp1286I 159 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 519 TGTACA T^GTACA
BsrGI 519 TGTACA T^GTACA
BsrI 251 ACTGG ACTGG(1/-1)
BssSI 671 CTCGTG CACGAG(-5/-1)
Bsu36I 94 CCTCAGG CC^T[ACGT]AGG
CelII 121 GCTGAGC GC^T[ACGT]AGC
DraIII 1224 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 903 GACTGCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 370 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 94 CCTCAGG CC^T[ACGT]AGG
HgaI 539 GCGTC GACGC(5/10)
MamI 265 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 644 CAATTG C^AATTG
MslI 956 CACCTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 644 CAATTG C^AATTG
MwoI 516 GCTTGTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 689 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 736 ATTAAT AT^TAAT
SexAI 717 ACCAGGT A^CC[AT]GGT
SspBI 519 TGTACA T^GTACA
TaqI 960 TCGA T^CGA
TspRI 860 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 960 TCGA T^CGA
Van91I 689 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 711 CCAAGTACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 837 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccI 328 GTAGAC GT^[AC][GT]AC
1088 GTCTAC
AvaII 640 GGACC G^G[AT]CC
1114 GGACC
BalI 662 TGGCCA TGG^CCA
929 TGGCCA
BanI 1158 GGCACC G^G[CT][GA]CC
1180 GGCACC
BsrDI 690 CATTGC GCAATG(2/0)
933 CATTGC
BstYI 51 GGATCC [GA]^GATC[CT]
533 AGATCT
EaeI 662 TGGCCA [CT]^GGCC[GA]
929 TGGCCA
MflI 51 GGATCC [GA]^GATC[CT]
533 AGATCT
MluNI 662 TGGCCA TGG^CCA
929 TGGCCA
MscI 662 TGGCCA TGG^CCA
929 TGGCCA
SmlI 1237 CTCAAG C^T[CT][GA]AG
1288 CTCAAG
StuI 988 AGGCCT AGG^CCT
1241 AGGCCT
XhoII 51 GGATCC [GA]^GATC[CT]
533 AGATCT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MvaI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I