Restriction map for
pFN21AE4428
(Circular) (Six-base) MAPSORT of: pFN21AE4428.seq Check: 9307 from: 1 to: 6040
pFN21AE4428 6040 bp
With 182 enzymes: SgfI *
March 28, 2012 14:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 3094 3383 1937
Size: 397 760 289 4594
Fragments arranged by size:
4594 760 397 289
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2187 2861 2974 4103 5487 5755 1632
Size: 402 153 674 113 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 674 402 268 153 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 2380 4769 2380
Size: 2389 3651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2029 2061 2029
Size: 32 6008
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2845 3349 3575 3727 5836 1493
Size: 1352 504 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 504 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2742 4594 160
Size: 872 1710 1852 1606
Fragments arranged by size:
1852 1710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 1870
Size: 73 1422 4545
Fragments arranged by size:
4545 1422 73
BamHI G'GATC_C
Cuts at: 2056 3370 3737 2056
Size: 1314 367 4359
Fragments arranged by size:
4359 1314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3163 3185 3361 4816 618
Size: 325 217 627 1376 22 176 1455 1842
Fragments arranged by size:
1842 1455 1376 627 325 217 176 22
BanII G_rGCy'C
Cuts at: 1391 3359 1391
Size: 1968 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2538 6035 2538
Size: 3497 2543
BlpI GC'TnA_GC
Cuts at: 2127 3457 2127
Size: 1330 4710
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 3771 4729
Size: 416 53 83 399 24 849 1505 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1753 1505 958 849 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 2949 3235 3301 4700 1239
Size: 1152 558 286 66 1399 2579
Fragments arranged by size:
2579 1399 1152 558 286 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 3278 4165 5030 5328 3227
Size: 51 887 865 298 3939
Fragments arranged by size:
3939 887 865 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2582 4703 882
Size: 617 1083 2121 2219
Fragments arranged by size:
2219 2121 1083 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 2274 6034 2274
Size: 3760 2280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 1186
Size: 1414 4626
BseYI C'CCAG_C
Cuts at: 3282 3325 5243 5739 3282
Size: 43 1918 496 3583
Fragments arranged by size:
3583 1918 496 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 4101 4186 5257 2168
Size: 1933 85 1071 2951
Fragments arranged by size:
2951 1933 1071 85
BsmI GAATG_Cn'
Cuts at: 2565 3551 3644 2565
Size: 986 93 4961
Fragments arranged by size:
4961 986 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 3359 4101 4186 5257 1391
Size: 399 378 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 378 85
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3395 844
Size: 2551 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 2937 4535 4709 65
Size: 1183 1446 243 1598 174 1396
Fragments arranged by size:
1598 1446 1396 1183 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2524 96
Size: 2428 3612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2676 4100 5112 2676
Size: 1424 1012 3604
Fragments arranged by size:
3604 1424 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 2665 2967 3259 2665
Size: 302 292 5446
Fragments arranged by size:
5446 302 292
BstYI r'GATC_y
Cuts at: 1972 2056 2538 3370 3737 4122 4139 5591 5677
Size: 84 482 832 367 385 17 1452 86
Cuts at: 5677 5689 6035 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 832 482 385 367 346 86
84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2100 2100
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 2004 2159 2427 3115 3189 513
Size: 552 939 155 268 688 74 3364
Fragments arranged by size:
3364 939 688 552 268 155 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 3234 3234
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2914 5047 809
Size: 507 1598 2133 1802
Fragments arranged by size:
2133 1802 1598 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2934 3487
Size: 54 1425 50 275 18 837 267 553
Cuts at: 3487 4376 6005 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 837 553 275 267 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2912 4627 5001 5147 387
Size: 1064 138 1323 1715 374 146 1280
Fragments arranged by size:
1715 1323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2187 2391 2861 2949 2974 3235
Size: 393 402 153 204 470 88 25 261
Cuts at: 3235 3301 4103 4700 5487 5755 1239
Size: 66 802 597 787 268 1524
Fragments arranged by size:
1524 802 787 597 470 402 393 268
261 204 153 88 66 25
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2507 3319 2507
Size: 812 5228
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 4681 4713 4755
Size: 32 135 32 794 32 2283 32 42
Cuts at: 4755 4787 1373
Size: 32 2626
Fragments arranged by size:
2626 2283 794 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 2649 3639 2649
Size: 990 5050
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2936 10
Size: 54 1425 343 837 267 3114
Fragments arranged by size:
3114 1425 837 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2965 3998 4357 4516 518
Size: 648 1799 1033 359 159 2042
Fragments arranged by size:
2042 1799 1033 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3456 4133 5281 5526 1606
Size: 1850 677 1148 245 2120
Fragments arranged by size:
2120 1850 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2427 3115 3189 513
Size: 552 939 423 688 74 3364
Fragments arranged by size:
3364 939 688 552 423 74
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2700 1108
Size: 1592 4448
PfoI T'CCnGG_A
Cuts at: 3317 3317
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SexAI A'CCwGG_T
Cuts at: 2722 2722
Size: 6040
SfcI C'TryA_G
Cuts at: 826 1045 3388 4523 5204 5395 826
Size: 219 2343 1135 681 191 1471
Fragments arranged by size:
2343 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 3293 4144 5045 5307 820
Size: 197 926 1299 51 851 901 262 1553
Fragments arranged by size:
1553 1299 926 901 851 262 197 51
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 2995 3248 1517
Size: 1478 253 4309
Fragments arranged by size:
4309 1478 253
StyI C'CwwG_G
Cuts at: 513 1065 2004 2427 2641 3115 3189 3479 513
Size: 552 939 423 214 474 74 290 3074
Fragments arranged by size:
3074 939 552 474 423 290 214 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 2524 2832
Size: 274 80 33 51 1443 42 505 308
Cuts at: 2832 4286 5998 96
Size: 1454 1712 138
Fragments arranged by size:
1712 1454 1443 505 308 274 138 80
51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2723 2723
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 2846 4169 2846
Size: 1323 4717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI