Restriction map for
pFN21AE4428
(Linear) (Six-base) MAPSORT of: KIEE4428.seq Check: 743 from: 1 to: 1335
KIEE4428.orf
With 182 enzymes: SgfI *
March 22, 2012 17:59 ..
AccI GT'mk_AC
Cuts at: 0 329 1089 1335
Size: 329 760 246
Fragments arranged by size:
760 329 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 182 856 969 1335
Size: 29 153 674 113 366
Fragments arranged by size:
674 366 153 113 29
AhdI GACnn_n'nnGTC
Cuts at: 0 375 1335
Size: 375 960
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 24 56 1335
Size: 24 32 1279
Fragments arranged by size:
1279 32 24
ApoI r'AATT_y
Cuts at: 0 840 1335
Size: 840 495
AseI AT'TA_AT
Cuts at: 0 737 1335
Size: 737 598
BamHI G'GATC_C
Cuts at: 0 51 1335
Size: 51 1284
BanI G'GyrC_C
Cuts at: 0 1158 1180 1335
Size: 1158 22 155
Fragments arranged by size:
1158 155 22
BglII A'GATC_T
Cuts at: 0 533 1335
Size: 533 802
BlpI GC'TnA_GC
Cuts at: 0 122 1335
Size: 122 1213
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 1335
Size: 261 1074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1335
Size: 147 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 944 1230 1296 1335
Size: 386 558 286 66 39
Fragments arranged by size:
558 386 286 66 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1273 1335
Size: 1222 51 62
Fragments arranged by size:
1222 62 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 577 1335
Size: 577 758
BsaBI GATnn'nnATC
Cuts at: 0 269 1335
Size: 269 1066
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 1335
Size: 595 740
BseYI C'CCAG_C
Cuts at: 0 1277 1320 1335
Size: 1277 43 15
Fragments arranged by size:
1277 43 15
BsiHKAI G_wGCw'C
Cuts at: 0 163 1335
Size: 163 1172
BsmI GAATG_Cn'
Cuts at: 0 560 1335
Size: 560 775
Bsp1286I G_dGCh'C
Cuts at: 0 163 1335
Size: 163 1172
BsrDI GCAATG_nn'
Cuts at: 0 689 932 1335
Size: 689 243 403
Fragments arranged by size:
689 403 243
BsrGI T'GTAC_A
Cuts at: 0 519 1335
Size: 519 816
BssSI C'ACGA_G
Cuts at: 0 671 1335
Size: 671 664
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 962 1254 1335
Size: 660 302 292 81
Fragments arranged by size:
660 302 292 81
BstYI r'GATC_y
Cuts at: 0 51 533 1335
Size: 51 482 802
Fragments arranged by size:
802 482 51
Bsu36I CC'TnA_GG
Cuts at: 0 95 1335
Size: 95 1240
BtgI C'CryG_G
Cuts at: 0 154 422 1110 1184 1335
Size: 154 268 688 74 151
Fragments arranged by size:
688 268 154 151 74
DraIII CAC_nnn'GTG
Cuts at: 0 1229 1335
Size: 1229 106
DrdI GACnn_nn'nnGTC
Cuts at: 0 909 1335
Size: 909 426
EaeI y'GGCC_r
Cuts at: 0 662 929 1335
Size: 662 267 406
Fragments arranged by size:
662 406 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 907 1335
Size: 907 428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 182 386 856 944 969 1230 1296
Size: 29 153 204 470 88 25 261 66
Cuts at: 1296 1335
Size: 39
Fragments arranged by size:
470 261 204 153 88 66 39 29
25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 502 1314 1335
Size: 502 812 21
Fragments arranged by size:
812 502 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 1335
Size: 361 32 942
Fragments arranged by size:
942 361 32
MfeI C'AATT_G
Cuts at: 0 644 1335
Size: 644 691
MscI TGG'CCA
Cuts at: 0 664 931 1335
Size: 664 267 404
Fragments arranged by size:
664 404 267
MslI CAynn'nnrTG
Cuts at: 0 960 1335
Size: 960 375
NcoI C'CATG_G
Cuts at: 0 422 1110 1184 1335
Size: 422 688 74 151
Fragments arranged by size:
688 422 151 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 695 1335
Size: 695 640
PfoI T'CCnGG_A
Cuts at: 0 1312 1335
Size: 1312 23
SexAI A'CCwGG_T
Cuts at: 0 717 1335
Size: 717 618
SmlI C'TyrA_G
Cuts at: 0 1237 1288 1335
Size: 1237 51 47
Fragments arranged by size:
1237 51 47
StuI AGG'CCT
Cuts at: 0 990 1243 1335
Size: 990 253 92
Fragments arranged by size:
990 253 92
StyI C'CwwG_G
Cuts at: 0 422 636 1110 1184 1335
Size: 422 214 474 74 151
Fragments arranged by size:
474 422 214 151 74
TatI w'GTAC_w
Cuts at: 0 14 519 827 1335
Size: 14 505 308 508
Fragments arranged by size:
508 505 308 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 718 1335
Size: 718 617
XmnI GAAnn'nnTTC
Cuts at: 0 841 1335
Size: 841 494
Enzymes that do cut:
AccI AcuI AhdI AloI ApoI AseI BamHI BanI
BglII BlpI BmrI BplI BpmI BpuEI BsaI BsaBI
BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BssSI
BstXI BstYI Bsu36I BtgI DraIII DrdI EaeI EciI
Eco57MI FalI Hin4I MfeI MscI MslI NcoI PflMI
PfoI SexAI SmlI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmtI Bpu10I BsaAI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EarI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI