################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccII AciI AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FauI Fnu4HI FseI FspI HaeII HhaI HinP1I HindIII HpaI ItaI KasI KpnI KspI MamI MluI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI TaqI TfiI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 51 GTATAC GT^[AC][GT]AC AccIII 1140 TCCGGA T^CCGGA AcyI 431 GGCGTC G[GA]^CG[CT]C AfaI 614 GTAC GT^AC AflIII 986 ACATGT A^C[GA][CT]GT AlwNI 1034 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 264 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 168 GTGCTC G[AT]GC[AT]^C AvaII 290 GGTCC G^G[AT]CC AvrII 1153 CCTAGG C^CTAGG BalI 900 TGGCCA TGG^CCA BanI 67 GGTGCC G^G[CT][GA]CC BbsI 331 GTCTTC GAAGAC(2/6) BclI 870 TGATCA T^GATCA BcnI 497 CCGGG CC^[GC]GG BglII 1173 AGATCT A^GATCT BlnI 1153 CCTAGG C^CTAGG BmyI 168 GTGCTC G[AGT]GC[ACT]^C BpuAI 331 GTCTTC GAAGAC(2/6) BsaHI 431 GGCGTC G[GA]^CG[CT]C BsaWI 1140 TCCGGA [AT]^CCGG[AT] BseAI 1140 TCCGGA T^CCGGA BseRI 858 GAGGAG GAGGAG(10/8) BsiHKAI 168 GTGCTC G[AT]GC[AT]^C BsiYI 951 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 951 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 168 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 613 TGTACA T^GTACA BspEI 1140 TCCGGA T^CCGGA BspHI 281 TCATGA T^CATGA BspLU11I 986 ACATGT A^CATGT BsrDI 78 CATTGC GCAATG(2/0) BsrGI 613 TGTACA T^GTACA Bst1107I 51 GTATAC GTA^TAC BstEII 1011 GGTTACC G^GT[ACGT]ACC BstPI 1011 GGTTACC G^GT[ACGT]ACC BstZ17I 51 GTATAC GTA^TAC DsaI 362 CCATGG C^C[GA][CT]GG EaeI 900 TGGCCA [CT]^GGCC[GA] EcoO65I 1011 GGTTACC G^GT[ACGT]ACC EcoRI 268 GAATTC G^AATTC FbaI 870 TGATCA T^GATCA HgaI 432 GCGTC GACGC(5/10) Hin1I 431 GGCGTC G[GA]^CG[CT]C HincII 42 GTCAAC GT[CT]^[GA]AC HindII 42 GTCAAC GT[CT]^[GA]AC HphI 180 GGTGA GGTGA(8/7) MaeII 531 ACGT A^CGT MfeI 609 CAATTG C^AATTG MluNI 900 TGGCCA TGG^CCA MroI 1140 TCCGGA T^CCGGA MscI 900 TGGCCA TGG^CCA MunI 609 CAATTG C^AATTG NciI 497 CCGGG CC^[GC]GG NcoI 362 CCATGG C^CATGG NspI 986 ACATGT [GA]CATG^[CT] RcaI 281 TCATGA T^CATGA RsaI 614 GTAC GT^AC SapI 403 GCTCTTC GCTCTTC(1/4) SfcI 15 CTGTAG C^T[GA][CT]AG SpeI 105 ACTAGT A^CTAGT SspBI 613 TGTACA T^GTACA Tsp45I 674 GTCAC ^GT[GC]AC XmnI 264 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BamHI 443 GGATCC G^GATCC 1166 GGATCC BsmFI 499 GGGAC GGGAC(10/14) 596 GTCCC BsmI 824 GCATTC GAATGC(1/-1) 940 GCATTC Cac8I 1123 GCCAGC GC[ACGT]^[ACGT]GC 1127 GCAAGC DdeI 171 CTCAG C^T[ACGT]AG 1177 CTAAG DraII 947 AGGGCCT [GA]G^G[ACGT]CC[CT] 1060 AGGCCCT EarI 404 CTCTTC CTCTTC(1/4) 426 GAAGAG EcoO109I 947 AGGGCCT [GA]G^G[ACGT]CC[CT] 1060 AGGCCCT EcoT14I 362 CCATGG C^C[AT][AT]GG 1153 CCTAGG HapII 497 CCGG C^CGG 1141 CCGG HinfI 843 GACTC G^A[ACGT]TC 1252 GAGTC HpaII 497 CCGG C^CGG 1141 CCGG Ksp632I 404 CTCTTC CTCTTC(1/4) 426 GAAGAG MslI 541 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1029 CACAACAGTG MspI 497 CCGG C^CGG 1141 CCGG PleI 843 GACTC GAGTC(4/5) 1252 GAGTC SmlI 90 CTCAAG C^T[CT][GA]AG 1133 CTCAAG StyI 362 CCATGG C^C[AT][AT]GG 1153 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BsaJI BsrI BssKI BstF5I BstNI BstYI Cfr13I DpnII EcoRII FokI HaeIII MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp509I TspRI XhoII